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Amplification of capsid gene of European brown hare syndrome virus by reserve transcriptase - polymerase chain reaction

100%

Chrobocinska M.

Bulletin of the Veterinary Institute in Puławy

1998

tom 42

nr 2

The reverse transcriptase-polymerase chain reaction (RT-PCR) was used to amplify the first part or full capsid gene (VP60) of EBHSV and RHDV. The fragment covered full capsid gene and the most part of gene contained in ORF2 of EBHSV was also obtained. The specificity of PCR products was checked by digestion with restriction enzymes.

Restriction enzyme analysis of the capsid protein gene of different strains of European brown hare syndrome virus

100%

Chrobocinska M.

Bulletin of the Veterinary Institute in Puławy

2001

tom 45

nr 2

Eight Polish strains of European brown hare syndrome virus, isolated in 1992-93, 1998 and 2001, as well as 2 standard strains: French (F/st) and Italian (It/st) were analysed. The fragments of genome, including the capsid protein gene, were amplified by RT-PCR or N-PCR and digested by endonucleases: Xho I, Sst I, BamH I, Sma I, Hpa II and BsuR I. The results of restriction enzyme digestion were compared with restriction maps, obtained after computer analysis of nucleotide sequences of other European strains, available in GenBank. The results of analysis of Polish strains, isolated in different years, revealed the changes in restriction profiles between them and other European strains. The 3 out of 5 Polish strains detected in 1992-93 demonstrated the same restriction profiles, similar to French "GD" strain. The two other strains revealed, for same endonucleases, restriction patterns similar to ltalian "BS89" or German strains. The strains isolated in 1998 and 2001 demonstrated higher differences in restriction profiles in comparison with Polish and other European strains previously detected. These changes in nucleotide scquences of the part of genome studied, indicate the diversity of EBHSV.

Analysis of the fragment of capsid protein gene sequences of Polish strains of European brown hare syndrome virus

84%

Chrobocinska M.

Bulletin of the Veterinary Institute in Puławy

2002

tom 46

nr 2

Ograniczanie wyników

3 Bulletin of the Veterinary Institute in Puławy

3 Chrobocinska M.

1 2002

1 2001

1 1998

Wyniki wyszukiwania

Amplification of capsid gene of European brown hare syndrome virus by reserve transcriptase - polymerase chain reaction

Restriction enzyme analysis of the capsid protein gene of different strains of European brown hare syndrome virus

Analysis of the fragment of capsid protein gene sequences of Polish strains of European brown hare syndrome virus