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The effect of culture medium formulation on Pinus nigra somatic embryogenesis

100%

Salaj T., Fraterova L., Carach M., Salaj J.

Dendrobiology

2014

tom 71

The potential of immature zygotic embryos to produce embryogenic tissue was tested using culture media differed in inorganic salt composition as well as plant growth regulator content. Explants cultured on DCR as well as MLV media gave the highest initiation percentages with maximum values of 8.8% (MLV) to 10.4% (DCR). Plant growth regulators content (standard or reduced) had no profound effect on embryogenic tissue initiation. Somatic embryo maturation as well as germination was dependent on the cell line and salt composition of the medium. Plantlet regeneration occurred in three cell lines out of five tested. During the maturation process profound changes occurred in the internal organisation of somatic embryos such differentiation of root meristem in precotyledonary somatic embryos and formation of provascular strands in cotyledonary somatic embryos.

Intra- and interpopulation relationships and taxonomic status of Pinus nigra Arnold in Croatia according to morphology and anatomy of needles

80%

Liber Z., Nikolic T., Mitic B.

Acta Societatis Botanicorum Poloniae

2002

tom 71

nr 2

Ograniczanie wyników

1 Acta Societatis Botanicorum Poloniae

1 Dendrobiology

1 Carach M.

1 Fraterova L.

1 Liber Z.

1 Mitic B.

1 Nikolic T.

1 Salaj J.

1 Salaj T.

1 2014

1 2002

Wyniki wyszukiwania

The effect of culture medium formulation on Pinus nigra somatic embryogenesis

Intra- and interpopulation relationships and taxonomic status of Pinus nigra Arnold in Croatia according to morphology and anatomy of needles