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Antifungal activity of water soluble exudates of pea leaves was assayed. Exudates of different nodal leaves were collected by dipping them in sterilized distilled water for different length of time and conidial germination of four non-pathogenic to pea fungi (Alternaria solani, Curvularia lunata, Helminthosporium penniseti and H. echinocloa) and one pea pathogenic to pea fungus (Erysiphe pisi) was observed in the collected exudates. The leaf exudates inhibited differently conidial germination and A. solani and C. lunata were found highly susceptible. Conidial germination on exudate-depleted pea leaves showed better germination of E. pisi than on non-depleted leaves. Bipolar germination was the highest on II and III nodal leaves dipped in distilled water for 18 h while after 24 h it was the highest on I nodal leaves following dipping in distilled water. Similarly, conidial germination of E. pisi was observed on different nodal leaves of pea of different age. It was very clear that young leaves did not support germination at all and on the same nodal leaves conidia germinated after the leaves became older. No germination was observed on less than 20-day-old leaves. Moreover, 30-day-old leaves were found to be highly susceptible as bipolar germination of E. pisi conidia was maximum on such leaves and did not increase with aging.
Powdery mildew (Erysiphe pisi Syd.) significantly reduces the yield and quality of pea all over the world. Screening of a broad range of germplasm revealed three highly resistant genotypes (Fallon, PS99102238 and PS0010128); eleven (Shawnee, Lifter, Franklin, PS610152, PS810240, PS710048, PS610324, PS810191, CGN3273, CGN3272, and PS9910188) showed symptoms after inoculation but the infection was not severe and recovery was rapid. Powdery mildew caused 86% loss to the germplasm, and the severity of the disease was associated with various phases. The pathogen inhibits seed development in the pod. Severe natural infection is expected to eliminate susceptible germplasm, some of which may have valuable, unique characteristics. The screening data were used to explore the relationship between susceptible and resistant genotypes, and between genetic diversity and geographic patterns. Seed protein assays did not sort genotypes by geographic pattern or disease resistance. It is suggested to transfer genes conferring disease resistance and economic yield to one genotype.
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