Wyniki wyszukiwania

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Surface coat differences between invasive Entamoeba histolytica and non-invasive Entamoeba dispar

100%

Gonzalez-Robles A., Chavez-Munguia B., Salazar-Villatoro L., Lagunes-Guillen A., Hernandez-Ramirez V. I., Talamas-Rohana P., Martinez-Palomo A.

Acta Protozoologica

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2015

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tom 54

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nr 3

2

Molecular diagnosis of Entamoeba spp. versus microscopy in the Great Cairo

84%

Roshdy M. H., El-Kader N. M. A., Ali-Tammam M., Fuentes I., Mohamed M. M., El-Sheikh N. A., Rubio J. M.

Acta Parasitologica

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2017

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tom 62

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nr 1

3

Prevalence and molecular differentiation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in Egypt

84%

Abozahra R., Mokhles M., Baraka K.

Acta Parasitologica

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2020

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tom 65

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nr 4

4

Differential detection of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in fecal samples by nested PCR in the United Arab Emirates (UAE)

67%

ElBakri A., Samie A., Ezzedine S., Odeh R. A.

Acta Parasitologica

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2013

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tom 58

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nr 2

Amoebiasis is one of the most important infectious diseases afflicting mainly tropical and subtropical countries. This study was carried out in the Sharjah Emirate, UAE in order to accurately detect and differentiate Entamoeba histolytica, Entamoeba dispar and E. moshkovskii in fecal samples collected from the Sharjah municipality public health clinic by ELISA and nested polymerase chain reaction (PCR). One hundred and twenty specimens were examined and the PCR was positive for E. histolytica, E. dispar and E. moshkovskii (collectively referred to as Entamoeba complex) in 19.2% (23 out of 120). Of those, 10% (12/120) were mono — infection with E. histolytica; 2.5% (3/120) with E. dispar; and 2.5% (3/120) E. moshkovskii. The nested PCR also detected mixed infections by both E. histolytica and E. dispar in 3.3% (4/120) and E. dispar and E. moshkovskii in 0.8% (1/120). The TechLab ELISA kit failed to detect E. histolytica in any of the E. histolytica PCR positive samples. Overall, the percentage of E. histolytica including those found in mixed infections was 13.3% (16/120). Compared to nested PCR, microscopy was found to have an overall sensitivity of 52.2% and a specificity of 75.2% for detection of Entamoeba complex. The present study indicates that E. histolytica is present in the UAE with an average incidence rate of 13.3%. However, larger studies need to be conducted in order to confirm these findings. We propose the use of PCR in both the routine diagnosis of amoebiasis and epidemiological survey in the UAE.

5

Molecular differentiation of Entamoeba histolytica and Entamoeba dispar from stool and culture samples obtained from Polish citizens infected in Tropics and in Poland

67%

Myjak P., Kur J., Pietkiewicz H., Kotlowski A., Nahorski W., Szostakowska B.

Acta Protozoologica

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2000

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tom 39

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nr 3

6

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Zastosowanie technik molekularnych do wykrywania i/lub identyfikacji pasozytow i grzybow u ludzi i zwierzat oraz patogenow przenoszonych przez kleszcze. Cz.1

59%

Myjak P., Majewska A. C., Bajer A., Sinski E., Wedrychowicz H., Golab E., Budak A., Stanczak J.

Wiadomości Parazytologiczne

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2001

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tom 47

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nr 3

433-455

After a long period of using basic microscopic, immunological and biochemical methods for diagnosis, rapid development of nucleic acids investigation enabled introduction of specific and sensitive methods of detection of pathogenic agents on the molecuiar level. Among others, polymerase chain reaction (PCR), discovered in mid of 80'ies and then automatized, offered an attractive alternative to conventional testing systems. In this paper we describe reliable diagnostic tests widely used in the world, including Poland, and capable of detecting different disease agents as parasites and fungi in clinical specimens and pathogens of emerging zoonotic diseases in ticks. The possibilities of using molecular methods for determination of Plasmodium falciparum drug resistance is also discussed. Moreover, the report offers information concerning kinds of molecular tests and institutions in which there are executed.

Ograniczanie wyników

Surface coat differences between invasive Entamoeba histolytica and non-invasive Entamoeba dispar

Molecular diagnosis of Entamoeba spp. versus microscopy in the Great Cairo

Prevalence and molecular differentiation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in Egypt

Differential detection of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in fecal samples by nested PCR in the United Arab Emirates (UAE)

Molecular differentiation of Entamoeba histolytica and Entamoeba dispar from stool and culture samples obtained from Polish citizens infected in Tropics and in Poland

Zastosowanie technik molekularnych do wykrywania i/lub identyfikacji pasozytow i grzybow u ludzi i zwierzat oraz patogenow przenoszonych przez kleszcze. Cz.1