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Combined morphological and molecular techniques were used to characterize variation in Elymus repens. We studied the morphological variability of E. repens in relation to the degree of its genetic differentiation, in order to unravel the causes of conspicuous intraspecific morphological variation. Four populations of E. repens from different habitats were analyzed for 35 morphological characters, and their genetic differentiation was assessed by Amplified Fragment Length Polymorphism (AFLP). Four pairs of selective primers were used to detect a total of 279 AFLP bands, of which 104 (37.28%) were polymorphic between populations. Cluster analysis based on AFLP fingerprint data showed that individuals were arranged in population-specific groups. The analyses of variance (ANOVA and AMOVA) indicated significant morphological and genetic differentiation among populations (P<0.01). This study revealed low levels of AFLP variation, which suggests that conspicuous morphological variation of E. repens is caused by plasticity. E. repens is an evolutionarily young species, of hybrid origin, in which microevolutionary processes continue. This study showed that common analysis of genetic diversity and morphology is a powerful tool in low-level taxonomy.
Data on share of grasses in the flora of selected 65 gravel-pits of the Siedlecka Upland as well as their ecological characterisation are presented in the paper. The Poaceae is one of the most species-rich families found in these localities. Among 60 grass species the most frequent include Arrhenatherum elatius, Dactylis glomerata subsp. glomerata and Elymus repens. The domination of meadow species from classes Molinio-Arrhenatheretea and Trifolio fragiferae-Agrostietalia stoloniferae as well as synanthropic grasses from classes Stellarietea mediae and Agropyretea intermedio-repentis was observed. The dominant life form of grasses are hemicryptophytes. Native species prevail over anthropophytes in the studied objects. This paper also presents the results of analysis of the flora based on ecological indicator values [21].
Inter- and intrapopulational morphological variability of Elymus repens, a cool-season peren-nial grass and a tenacious weed that spreads both by seeds and rhizomes forming a thick and stout net, was examined. Multivariate statistical analysis of 48 initial morphological characters obtained from 44 population samples (1180 specimens) collected in different habitats revealed the conspicuous variation within E. repens. Principal Component Analysis revealed three extremes of the morphological gradient that were referred to as three varieties of E. repens: var. repens, var. aristatus and var. subulatus, not correlated with the habitat type or geographi-cal location. Canonical Discriminant Analysis proved the taxonomic usefulness of characters such as awned or awnless glumes and lemmas as well as the shape of glumes for variety di-stinction. Results of morphological analysis, in relation to the low level of genetic variation showed in the previous studies, suggest that the extensive morphological variation of E. re-pens can be caused by its plasticity where different morphological patterns are realized on the same genome basis. Additionally, the open-pollinated system of mating results in new combi-nations of morphological characters of plants that further reproduce vegetatively by rhizomes. The nomenclature, taxonomic descriptions and a key to the three varieties are provided.
Field experiments were conducted to determine quizalofop-P-tefuryl (Pantera 040 EC) efficacy for control of couchgrass (E. repens) at crop rotation peas-spring cercals. The results showed that herbicides applied at 4-8 leaf stage of E. repens enhanced weed control effect. Subsequent treatments i.e. at 10- 12 leaf stage of E. repens were less effective. In the following years, the yield of spring cereals depended on efficacy of E. repens control in forecrop i.e. pea. Spring cereals (wheat and barley) gave better yield when higher level of E. repens control was observed.
In the experiments conducted during 2007–2009, 8 herbicides (including 3 graminecides) were examined to evaluate their usefulness on Miscanthus giganteus plantations. The best effect in weed control (Apera spica-venti and Echinochloa crus-galli) was achieved applying herbicides Apyros 75 WG and Callisto 100 EC. By contrast, the best results in control on a plantation dominated by Elymus repens were obtained after applying graminecide Perenal 104 EC.
The roadside vegetation in some counties of north England (north and west Yorkshire) was studied to determine the community structure according to the British National Vegetation Classification (NVC) and main environmental factors influencing its composition. The data from Phytosociological survey (699 quadrats) and from the physico-chemical analyses of 233 soil samples from 35 sites were obtained. Both the classification (TWINSPAN & MATCH) and ordination programs (Canonical Correspondence Analysis) were used. The roadside vegetation is mainly dominated by few grasses (Arrhenatherum elatius, Festuca rubra, Dactylis glomerata, Lolium perenne, Poa trivialis, Elymus repens, Holcus lanatus) and their associated herbs (Cirsium arvense, Heracleum sphondylium, Urtica dioica). Five NVC Mesotrophic grassland communities (Arrhenatheretum elatioris community MG1, Lolium perenne-Cynosurus cristatus grassland MG6, Lolium perenne leys MG7, Holcus lanatus-Deschampsia cespitosa grassland MG9, Festuca rubra-Agrostis stolonifera-Potentilla anserina grassland MG11) and one Upland Festuca ovinaAgrostis capillaris-Galium saxatile grassland, U4 were identified which in general, exhibited good fit with the typical NVC units. Altitude, pH, potassium, sodium and road age were found to be the main variables affecting the roadside vegetation. By relating the floristic composition with ecological characteristics of the roadside verges, three kinds of pattern of variation are observed. The first pattern is related to regional or geographical characteristics and the second pattern of variation exists across the width of the road verges showing a zonal pattern of plant distribution. The third scale of pattern is active at the local level including micro-environmental conditions, e.g., local edaphic variables.
The mechanisms of vegetation zonation were determined in order to provide an advice for restoration of natural saline habitats. Field experiments were conducted to examine the response of mature plants to different edaphic conditions. Three dominant species Salicornia europaea L., Puccinellia distans (L.) Parl. and Elymus repens (L.) Golud, characteristic of distinct zones along the salinity gradient (ECe 28.5–2.3 m Scm⁻¹) were studied. Results from a 2-year reciprocal transplant experiment demonstrated that species were restricted to every zone mostly by a salinity level. The obligatory halophyte S. europaea had optimal growth conditions at its home site. This is an opposite result to the one known from inland salt marshes of North America. A distinct growth limitation of transplants was observed in the P. distans and E. repens zones of lower salinity. Fewer individuals and lower aboveground biomass were recorded in the P. distans zone, whereas in the E. repens zone all seedlings died in the second year of observations. The glycophyte E. repens from the less saline site (ca 2.3 mS cm⁻¹) was strongly inhibited in the most saline S. europaea zone (15.8–28.5 mS cm⁻¹). Compared to the control transplants in the S. europaea zone it had shorter new shoots, fewer and shorter shoots, lower above-ground biomass and biomass of rhizomes. The P. distans transplants were markedly limited in the E. repens zone of lower salinity. Fewer and shorter new shoots, flowering shoots, lower above-ground biomass and biomass of grasses’ roots were noted in the transplants of this zone. Since P. distans was found in non-saline areas outside the investigated meadow this effect could not result from the salinity level but from E. repens interaction. The obtained results suggest that for restoration of natural saline habitats the most important is to keep or rebuild the original salinity level of soils. As the second point the control of strong competitors by cutting or grazing should be considered.
Hybrid speciation within the genus Elymus is analyzed and discussed. The supposed hybrid origin of Elymus x mucronatus between Elymus repens and Elymus hispidus was tested. In this aim, pollen viability and AFLP variation of putative progenitors and progenies from two natural populations were studied. Pollen fertility of hybrids ranging from 0 to 91.95% (average 59.50%) indicates the occurrence of F1 hybrids and backcross hybrids. Seven EcoRI/MseI AFLP primer combinations generated a total of 477 AFLP fragments (300 polymorphic) for 48 parental and hybrid individuals. UPGMA and principal coordinates analysis of the AFLP data set show that hybrids are closer to E. hispidus than to E. repens and suggest unidirectional introgression towards E. hispidus. AFLP fragments of the hybrid taxon were additively inherited from the parental species and no hybrid-specific bands were identified. AFLP analysis validates the hybrid status of E. ×mucronatus. Populations in which E. repens, E. hispidus and their hybrids co-occur still progress from early hybridization stages of F1 hybrids to an advanced hybridization stage of a well-established hybrid swarm. E. ×mucronatus is potentially self-sustaining by predominant vegetative reproduction by rhizomes and partial fertility leading to the development of mature seeds. We propose to consider Elymus ×mucronatus as a new species of hybrid origin in the Polish flora.
W doświadczeniu szklarniowym przeprowadzonym w 1999 roku określono wpływ wprowadzenia do gleby suchej masy pędów nadziemnych i korzeni bobiku, grochu polnego, łubinu żółtego, wyki siewnej, owsa, gryki, gorczycy białej i rzepaku ozimego na wzrost pędów nadziemnych (liczba, wysokość i sucha masa) perzu właściwego. Wymieszanie gleby z suchą masą wymienionych roślin uprawnych (w ilości 20, 40, 60 g na 550 g gleby) prowadziło do zmniejszenia liczby pędów perzu właściwego o 4,1-38,1%. Wysokość i sucha masa chwastu była niższa po dodaniu do gleby pędów nadziemnych owsa i największej ilości wyki siewnej (o 18,0-60,7%) oraz korzeni rzepaku ozimego i gryki (o 21,6-60,7%). Wprowadzenie do gleby pędów i korzeni pozostałych roślin uprawnych stymulowało wzrost perzu właściwego, szczególnie zaś po dodaniu najmniejszych ilości pędów bobiku, grochu polnego, łubinu żółtego, wyki jarej, gorczycy białej, rzepaku ozimego oraz korzeni wyki jarej i łubinu żółtego. Uzyskane wyniki mogą być wykorzystane w integrowanych programach ograniczania zachwaszczenia perzem właściwym.
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