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Indole-3-acetic acid production and effect on sprouting of yam [Dioscorea rotundata L.] minisetts by Bacillus subtilis isolated from culturable cowdung microflora

100%

Swain M. R., Naskar S. K., Ray R. C.

Polish Journal of Microbiology

2007

tom 56

nr 2

Bacillus subtilis strains (CM1-CM5) isolated from culturable (cowdung microflora were investigated for indole-3-acetic acid (IAA) production in nutrient broth (NB). All the strains tested produced IAA in NB; albeit in very low concentrations (0.09-0.37 mg/l). The addition of L-tryptophan (0.1-1.0 g/l) into NB substantially enhanced IAA production (6.1-31.5 folds) indicating that L-tryp-tophan was the precursor for IAA biosynthesis by these bacterial strains. Maximum IAA production was observed after 8 days of incubation (in late stationary phase of bacterial growth). The variation in IAA production was attributed to the genetic make up of these strains as evaluated by RAPD analysis of these isolates and B. subtilis type strain MTCC 441. Application of B. subtilis suspension (8 x 10⁹ CFU/ml) on the surface of yam (Dioscorea rotundata L.) minisetts increased the number of sprouts, roots and shoots length, root and shoot fresh weights and root: shoot ratio over those minisetts not treated with bacterial suspension. Fresh cowdung slurry treatment on yam minisetts also produced similar results as obtained with B. subtilis application.

Effects of calcium chloride on metabolism of salt-stressed Dioscorea rotundata

80%

Jaleel C. A., Gopi R., Gomathinayagam M., Panneerselvam R.

Acta Biologica Cracoviensia. Series Botanica

2008

tom 50

nr 1

In a pot culture experiment, the effect of calcium chloride (CaCl2) as an ameliorating agent on sodium chloride (NaCl) stress was studied in Dioscorea rotundata plants. Plants were raised in pots and exposed to salinity stress (80 mM NaCl) with or without 5 mM CaCl2. NaCl-stressed plants showed decreased protein and total sugars, and increased free amino acid and proline content. When NaCl treatment was combined with CaCl2, overall plant metabolism was altered, with increased antioxidant enzyme activity, paving the way for partial amelioration of oxidative stress caused by salinity.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Polish Journal of Microbiology

1 Gomathinayagam M.

1 Gopi R.

1 Jaleel C. A.

1 Naskar S. K.

1 Panneerselvam R.

1 Ray R. C.

1 Swain M. R.

1 2008

1 2007

Wyniki wyszukiwania

Indole-3-acetic acid production and effect on sprouting of yam [Dioscorea rotundata L.] minisetts by Bacillus subtilis isolated from culturable cowdung microflora

Effects of calcium chloride on metabolism of salt-stressed Dioscorea rotundata