Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: Deinococcus geothermalis

Sortuj według:

Ogranicz wyniki do:

Effects of the polyhistidine tag on kinetics and other properties of trehalose synthase from Deinococcus geothermalis

100%

Panek A., Pietrow O., Filipkowski P., Synowiecki J.

Acta Biochimica Polonica

2013

tom 60

nr 2

Two recombinant trehalose synthases from Deinococcus geothermalis (DSMZ 11300) were compared. A significant influence of the artificial polyhistidine tag was observed in protein constitution. The recombinant trehalose synthase from D. geothermalis with His6-tag has a higher Km value of 254 mM, in comparison with the wild-type trehalose synthase (Km 170 mM), and displayed a lower activity of maltose conversion when compared to the wild type. Moreover, differences in properties like temperature, pH, thermal- and pH-stability were observed. Presence of the histidine tag caused a decrease of thermal resistance in case of trehalose synthase with His6-tag. These data confirmed a suggestion that the introduction of the histidine domain produces in some seldom cases undesirable changes in the protein.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Filipkowski P.

1 Panek A.

1 Pietrow O.

1 Synowiecki J.

1 2013

Wyniki wyszukiwania

Effects of the polyhistidine tag on kinetics and other properties of trehalose synthase from Deinococcus geothermalis