Wyniki wyszukiwania

1

Development of a method for extracting macrophages from zebrafish, Danio rerio and their use to assess stress

100%

Mustafa A., Dhawale S., Dhawale S.

Acta Ichthyologica et Piscatoria

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2008

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tom 38

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nr 1

Background. To assess stress levels in fishes, plasma cortisol levels are measured by radioimmuno assay and phagocytic activity is assessed using macrophages. However, the small size of some fishes makes it difficult to measure stress using these physiological and immunological indicators. In this study, we investigated the possibility of obtaining macrophages from zebrafish via whole body extractions by assessing the respiratory burst activity and phagocytic capacity of extracted cells and we studied the effects of temperature stress on zebrafish using the extracted macrophages. Materials and Methods. One hundred and fifty genetically pure zebrafish, Danio rerio (Hamilton, 1822), were randomly divided into three groups and placed in three different environments: optimal (28°C), warm (32°C), and cool (23°C). Using the newly developed extraction method described in this article macrophages were extracted from whole fish bodies and the phagocytic activity of these cells were assessed. Results. The method yielded enough macrophages to examine their respiratory burst activity and phagocytic capacity. Values obtained for experimental replicates were similar and the assessment measures were sensitive enough to detect differences in these parameters among fish maintained at three different temperatures for 2, 4, 6, and 8 weeks. These results suggest that macrophages can be successfully extracted using the whole body method and the extracted macrophages are useful for studying stress. Conclusion. The method of macrophage extraction described in this article is simple and rapid, and will enable researchers to study the effects of any stressors, environmental or pathogenic, on the non-specific immune response of fish.

2

Structural changes in the male gonads of Danio rerio (Teleostei: Cyprinidae) during reproductive cycle

100%

Huszno J., Lozowski B.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2008

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tom 50

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nr 1

3

Zebrafish as an animal model for McArdle disease

100%

Migocka-Patrzalek M., Kosieradzka A., Dubinska-Magiera M., Daczewska M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2016

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tom 58

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nr 1

4

Functional orthologs of human small heat shock protein HSPB8: Drosophila melanogaster Hsp67Bc and Danio rerio HSPB8

100%

Dubinska-Magiera M., Jablonska J., Posyniak E., Jagla K., Daczewska M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2016

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tom 58

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nr 1

5

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Mała rybka - wielkie wyzwania

100%

Korzeniowski P. J., Wiweger M.

Wszechświat

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2016

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tom 117

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nr 10-12

6

Danio pręgowany jako organizm modelowy w badaniach nad nowotworami

100%

Olszewski M., Pruszko M.

Wszechświat

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2016

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tom 117

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nr 10-12

7

Effects of subchronic exposure to atrazine on zebrafish (Danio rerio)

84%

Plhalova L., Blahova J., Mikulikova I., Stepanova S., Dolezelova P., Praskova E., Marsalek P., Skoric M., Pistekova V., Bedanova I., Svobodova Z.

Polish Journal of Veterinary Sciences

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2012

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tom 15

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nr 3

The aim of this study was to investigate the effects of subchronic exposure to atrazine on fish growth and the development of histopathological changes in selected organs (gill, kidney, liver) in Danio rerio. Juvenile growth tests were performed on D. rerio according to OECD method No. 215. For 28 days, fish at an initial age of 30 days were exposed to the environmental atrazine concentration commonly detected in Czech rivers (0.3 μg/L) and a range of sublethal concentrations of atrazine (3.0, 30.0 and 90.0 μg/L). The results showed decreasing growth rates and morphological changes in the liver (dystrophic lesions of hepatocytes) at 90.0 μg/L of atrazine. The environmental concentration of atrazine in Czech rivers did not have any effect on fish growth and development of histopathological changes in D. rerio. The value of NOEC was 30.0 μg/L and the value of LOEC was 90.0 μg/L.

8

Structural and ultrastructural studies of the notochord differentiation in zebrafish Danio rerio (Cyprinidae)

84%

Rupik W., Urbanska-Jasik D.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2008

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tom 50

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nr 1

9

Wpływ mykotoksyn wykrywanych w paszach na rozwój ryb Danio rerio

84%

Radko L., Jedziniak P., Jakubaszek A., Stypula-Trebas S., Posyniak A.

Pasze Przemysłowe

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2018

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tom 27

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nr 2

10

Okiem ryby - nowe spojrzenie na rozwój sieci neuronalnych i choroby neurorozwojowe

84%

Kedra M., Jaworski J., Zmorzynska J.

Wszechświat

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2016

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tom 117

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nr 10-12

11

Przyżyciowe badania nad funkcjonowaniem mózgu przy użyciu mikroskopii SPIM

84%

Bazala M., Jedrychowska J., Kuznicki J.

Wszechświat

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2016

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tom 117

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nr 10-12

12

Toxicity of azadirachtin on some biomarkers of oxidative stress in zebrafish, Danio rerio

84%

Sharma D. K., Ansari B. A.

Journal of Biology and Earth Sciences

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2014

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tom 04

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nr 2

13

Effect of two different Nodularia spumigena extracts on Danio rerio egg mortality and hatching time

84%

Tyminska A., Mazur-Marzec H., Graczyk M., Plinski M.

Oceanological and Hydrobiological Studies

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2005

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tom 34

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nr Suppl.3

14

Simultaneous accumulation of anatoxin-a and microcystins in three fish species indigenous to lakes affected by cyanobacterial blooms

67%

Pawlik-Skowronska B., Toporowska M., Rechulicz J.

Oceanological and Hydrobiological Studies

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2012

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tom 41

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nr 4

15

Preliminary experiments on use of zebrafish as a laboratory model for Giardia duodenalis infection

67%

Tysnes K. R., Jorgensen A., Poppe T., Midtlyng P. J., Robertson L. J.

Acta Parasitologica

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2012

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tom 57

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nr 1

Although Giardia duodenalis is considered a parasite of mammals, different genotypes have been identified as infecting several species of freshwater and marine fish in Australia. Establishment of G. duodenalis infection in common laboratory zebrafish (Danio rerio), could provide an excellent tool for a range of studies on Giardia. We conducted preliminary experiments to investigate this possibility. Zebrafish were inoculated with viable G. duodenalis cysts from two different Assemblages (A and D) using a modified oro-gastric tube. Direct microscopy and immunofluorescent antibody test were used to check for Giardia cysts/trophozoites in the intestine, and histology was performed on intestinal mucosa to evaluate possible pathological changes. Giardia cysts were successfully deposited in the zebrafish alimentary tract using a modified oro-gastric tube, and were maintained in the fish gut for at least 8 days. Although a single trophozoite was observed in one fish three days post-exposure, we were unable to demonstrate established, propagative infection under the conditions tested.

16

Effect of the synthetic pyrethroid Deltamethrin and the neem-based pesticide Achook on the reproductive ability of zebrafish, Danio rerio (Cyprinidae)

67%

Sharma D. K., Ansari B. A.

Archives of Polish Fisheries

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2010

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tom 18

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nr 3

Ograniczanie wyników

Development of a method for extracting macrophages from zebrafish, Danio rerio and their use to assess stress

Structural changes in the male gonads of Danio rerio (Teleostei: Cyprinidae) during reproductive cycle

Zebrafish as an animal model for McArdle disease

Functional orthologs of human small heat shock protein HSPB8: Drosophila melanogaster Hsp67Bc and Danio rerio HSPB8

Mała rybka - wielkie wyzwania

Danio pręgowany jako organizm modelowy w badaniach nad nowotworami

Effects of subchronic exposure to atrazine on zebrafish (Danio rerio)

Structural and ultrastructural studies of the notochord differentiation in zebrafish Danio rerio (Cyprinidae)

Wpływ mykotoksyn wykrywanych w paszach na rozwój ryb Danio rerio

Okiem ryby - nowe spojrzenie na rozwój sieci neuronalnych i choroby neurorozwojowe

Przyżyciowe badania nad funkcjonowaniem mózgu przy użyciu mikroskopii SPIM

Toxicity of azadirachtin on some biomarkers of oxidative stress in zebrafish, Danio rerio

Effect of two different Nodularia spumigena extracts on Danio rerio egg mortality and hatching time

Simultaneous accumulation of anatoxin-a and microcystins in three fish species indigenous to lakes affected by cyanobacterial blooms

Preliminary experiments on use of zebrafish as a laboratory model for Giardia duodenalis infection

Effect of the synthetic pyrethroid Deltamethrin and the neem-based pesticide Achook on the reproductive ability of zebrafish, Danio rerio (Cyprinidae)