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1

Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection

100%
Wisniewski M., Jaros S., Baska P., Cappello M., Wedrychowicz H.
Acta Parasitologica
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2013
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tom 58
|
nr 3
Hookworms are blood feeding intestinal nematodes that infect more than 500 million people and cause iron deficiency anemia. Infected children suffer from physical and cognitive growth retardation. Because of potential anthelminthic drug resistance, the need for vaccine development is urgent. Numerous antigens have been tested in animal models as vaccines against hookworm infection, but there is no effective human vaccine. We cloned a cDNA encoding Ancylostoma ceylanicum metalloprotease 6 (Acemep-6). Ace-MEP-6 is a protein with a predicted molecular mass of 101.87 kDa and based on computational analysis it is very likely to be engaged in food processing via hemoglobin digestion. Groups of hamsters were immunized with an Ace-mep-6 cDNA vaccine, either once or three times. Animals that were administered one dose developed high resistance (80%, p < 0.01) against challenge infection, whereas triple immunization resulted in no worm burden reduction. These results suggest that DNA vaccines can be powerful tools in ancylostomiasis control, although the mechanisms through which protection is conferred remain unclear.
2

Protective effect of pCI-neo vector-mediated DNA vaccine against Chlamydophila abortus in mice

100%
Li S., He C., Ling Y., Wang H., Zhao J., Yao B.
Bulletin of the Veterinary Institute in Puławy
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2008
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tom 52
|
nr 2
In the presented study, omp-1 gene coding main outer membrane protein of Chlamydophila abortus was cloned into pCI-neo and pcDNA3.1 as delivery vehicles for DNA vaccination. Thirty-six BALB/c mice were randomly assigned to three groups and inoculated intramuscularly with: 1) 100 µg of pCI-neo, 2) 100 µg of pCI-neo::MOMP, and 3) 100 µg of pcDNA3.1::MOMP. All animals were vaccinated three times at 14 d intervals. The results showed that mice given pCI-neo::MOMP developed a higher IgG antibody level, high T lymphocytes proliferations, and high titres of IFN-γ and IL-2, than mice given pcDNA3.1::MOMP, which induced moderate antibody levels, less T lymphocyte proliferations and lower cytokine levels. No significant difference of TNF-α was observed in above groups. Additionally, IgG2a and Ig2b were the predominant isotypes on day 44, suggesting a high level of Th1 stimulation. Mice given the pCI-neo::MOMP also elicited a higher chlamydial clearance and a better protection than mice with pcDNA3.1::MOMP did. Immunisation with pCI-neo::MOMP vaccine may provide novel ways for active immunisation strategy against Chlamydophila abortus.
3

Molecular cloning of enolase from Trichinella spiralis and the protective immunity in mice

84%
Zhang X., Xu L., Song X., Li X., Yan R.
Acta Parasitologica
|
2018
|
tom 63
|
nr 2
4

Evaluation of the immune response of male and female rats vaccinated with cDNA encoding a cysteine proteinase of Fasciola hepatica (FhPcW1)

84%
Wesolowska A., Norbury L. J., Januszkiewicz K., Jedlina L., Jaros S., Zawistowska-Deniziak A., Zygner W., Wedrychowicz H.
Acta Parasitologica
|
2013
|
tom 58
|
nr 2
Not only do males and females of many species vary in their responses to certain parasitic infections, but also to treatments such as vaccines. However, there are very few studies investigating differences among sexes following vaccination and infection. Here we demonstrate that female Sprague-Dawley rats vaccinated with cDNA encoding a recently discovered cysteine proteinase of Fasciola hepatica (FhPcW1) develop considerably lower liver fluke burdens after F. hepatica infection than their male counterparts. This is accompanied by differences in the course of their immune responses which involve different eosinophil and monocyte responses throughout the study as well as humoral responses. It is evident that host gender influences the outcome of parasitic infections after vaccination and research on both sexes should be considered when developing new treatments against parasites.
5

Schistosoma japonicum: the design and experimental evaluation of a multivalent DNA vaccine

84%
Li C., Yu L., Liu Z., Zhu L., Hu Y., Zhu M., Zhu X., Shi Y., Meng S.
Cellular and Molecular Biology Letters
|
2006
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tom 11
|
nr 4
The aim of this study was to construct and evaluate the immunity efficacy of the DNA multivalent vaccine pVIVO2SjFABP-23. The vaccine was constructed and produced as follows. Forty BALB/c mice were divided into four groups designated pVIVO2, pVIVO2Sj23, pVIVO2SjFABP and pVIVO2SjFABP-23. Each mouse was immunized with 100 µg of the corresponding plasmid DNA by intramuscular injection. 28 days postvaccination, the mice were challenged with S. japonicum cercariae, and the worm and egg burdens were determined 42 days post-challenge. Serum samples were collected from all the mice before and after vaccination and at the end of the experiment, and used for antibody detection. The IFN-γ and IL-4 levels were quantified in the supernatants of specifically stimulated spleen cells. The number of worms was reduced by 52%, 40% and 42% in mice respectively immunized with pVIVO2SjFABP-23, pVIVO2Sj23 or pVIVO2SjFABP. A respective 61%, 38% and 39% egg reduction was determined relative to those mice that only received the empty pVIVO2 plasmid. pVIVO2SjFABP-23 immunization increased IgG levels against SWAP and SEA. Increased IFN-γ levels were detected in the supernatant of specific stimulated spleen cells from mice immunized with the 3 different constructs. The multivalent DNA vaccine developed induced higher levels of protection than the two monovalent tested vaccines.
6

Perspectives of anti-H.pylori vaccination

84%
Saldinger P. F., Blum A. L., Corthesy-Theulaz I. E.
Journal of Physiology and Pharmacology. Supplement
|
1997
|
tom 48
|
nr 4
7

Protection of cattle against bovine leukemia virus [BLV] infection could be attained by DNA vaccination

67%
Brillowska A., Dabrowski S., Rulka J., Kubis P., Buzala E., Kur J.
Acta Biochimica Polonica
|
1999
|
tom 46
|
nr 4
The bovine leukemia virus (BLV) envelope gene encoding extracellular glycoprotein gp51 and transmembrane glycoprotein gp30 was cloned into a vehicle expression vector under the human cytomegalovirus (CMV) intermediate early promoter. The intramuscular injection of this plasmid vector generated a cellular immune response. Seven out of ten cows vaccinated with the DNA construct resisted a drastic challenge (500 BLV-infected lymphocytes as an infectious dose).
8

DNA vaccination against parasites: hope or mirage?

67%
Kofta W., Wedrychowicz H. Z.
Acta Parasitologica
|
1999
|
tom 44
|
nr 2
Experimental data obtained so far suggest that an efficacious anti-parasitic vaccine must either be completely effective against infective stage of a parasite or it must induce multi-stage, multi-immune response. Recently developed technology of DNA vaccination appears to offer the best prospect for the development of multivalent vaccines that will effectively activate both the humoral and cell mediated mechanisms of the immune system. The strength of the genetic vaccination lays in the continuous expression of the immunogen in the vaccinated organisms. From the evidences gathered so far it appears that the type of immune response elicited by DNA vaccination depends very much on the antigen used and on the way of the vaccine delivery. This paper presents advantages and disadvantages of vaccinating host organism with cDNA encoding for various microbial and parasitic antigens.
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