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1

An improved method for efficient isolation and purification of genomic DNA from filamentous cyanobacteria belonging to genera Anabaena, Nodularia and Nostoc

100%
Kaczynska A., Los M., Wegrzyn G.
Oceanological and Hydrobiological Studies
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2013
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tom 42
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nr 1
2

Evaluation of faecal flotation methods followed by species-specific PCR for detection of Echinococcus multilocularis in the definitive hosts

100%
Szell Z., Sreter-Lancz Z., Sreter T.
Acta Parasitologica
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2014
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tom 59
|
nr 2
Echinococcus multilocularis is one of the most pathogenic zoonotic parasites in the temperate and arctic region of the Northern Hemisphere. For estimating the potential risk of human infection in endemic areas, reliable antemortem methods are needed to detect the parasite in carnivore definitive hosts. The sensitivity of routine flotation techniques for detection of E. multilocularis eggs was found to be low (3–33%) depending on the flotation solution used (specific gravities = 1.3–1.4). An improved faecal flotation followed by a species-specific PCR is described with a sensitivity of 74% (95% CI = 62–84%) and a specificity of 100% (95% CI = 94–100%). These parameters are similar to those of the intestinal scraping technique (sensitivity = 78%, specificity = 100%). The sensitivity of the improved flotation was significantly higher (P < 0.0001) than that of routine flotation techniques. The costs of the method are similar or lower than those of other antemortem diagnostic methods. Based on these data, the method is suitable for surveys of domesticated and wild carnivores.
3

DNA-nuclear matrix interactions analyzed by cross-linking reactions in intact nuclei from avian liver

100%
Ferraro A., Eufemi M., Cervoni L., Altieri F., Turano C.
Acta Biochimica Polonica
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1995
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tom 42
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nr 2
To detect the interactions of DNA with the nuclear matrix proteins, DNA-protein cross-linkages were induced in intact nuclei from chicken liver by the use of ds-diammine dichloroplatinum. Methods have been devised for fast purification both of the proteins and of the DNA fragments involved in the cross-linked complexes. By Southern-Western blotting a number of matrix proteins isolated from the complexes have been shown to recognize specifically DNA sequences present in the cross-linked DNA fragments. This experimental approach not only allows to identify the nuclear matrix-DNA interactions existing in the nucleus before its disruption, but also provides a preparation of matrix proteins enriched in those species which are involved in such interactions and which can therefore be detected with high sensitivity.
4

Analysis of polymorphisms in the mediator complex subunit 13-like (MED13L) gene in the context of immune function and development of experimental arthritis

80%
Sardar S., Kanne K., Andersson A.
Archivum Immunologiae et Therapiae Experimentalis
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2018
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tom 66
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nr 5
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