Wyniki wyszukiwania

1

Biochemical characteristics of the chromatin-remodeling enzyme DDM1

100%

Brzeski J.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

2

Role of genomic imprinting in endosperm development

88%

Scott R. J., Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, U.K.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

3

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Exogenous steroid hormones stimulate full development of autonomous endosperm in Arabidopsis thaliana

88%

Rojek J., Pawelko L., Kapusta M., Naczk A., Bohdanowicz J.

Acta Societatis Botanicorum Poloniae

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2015

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tom 84

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nr 2

Most flowering plants, including important crops, require double fertilization to form an embryo and endosperm, which nourishes it. Independence from fertilization is a feature of apomictic plants that produce seeds, from which the plants that are clones of the mother plant arise. The phenomenon of apomixis occurs in some sexual plants under specific circumstances. Since the launch of a fertilization-independent mechanism is considered a useful tool for plant breeding, there have been efforts to artificially induce apomixis. We have been able to produce fertilization-independent endosperm in vitro in Arabidopsis over the last few years. This paper demonstrates the methods of improving the quality of the endosperm obtained using plant and mammalian steroid hormones. Additionally, it shows the study on the autonomous endosperm (AE) formation mechanism in vitro. This paper examines the effect of exogenous steroid hormones on unfertilized egg and central cell divisions in culture of unpollinated pistils of Arabidopsis Col-0 wild-type andfie-1 mutant. All media with hormones used (estrone, androsterone, progesterone, and epibrassinolide) stimulated central cell divisions and fertilization-independent endosperm development. The stages of AE development followed the pattern of Arabidopsis thaliana wild type after fertilization. Subsequent stages of AE were observed from 2-nuclear up to cellular with the most advanced occurring on medium with 24-epibrassinolide and progesterone. The significant influence of mammalian sex hormones on speed of AE development and differentiation was noticed. Using restriction analysis, the changes in methylation of FIE gene was established under in vitro condition. The authors of this paper showed that Arabidopsis thaliana has a high potency to fertilization-independent development.

4

The role of DNA methylation in cancer development

88%

Luczak M. W., Jagodzinski P. P.

Folia Histochemica et Cytobiologica

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2006

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tom 44

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nr 3

5

Methylation pattern analysis of the sequences conding the 18S and 15SrRNA genes in the genomic DNA of wheat callus tissues

88%

Zubek W., Dabrowska G., Szechynska M., Filek M.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

6

Demethylation of the sex-determining region Y gene promoter and incidence of disorder of sex development in cloned dog males

75%

Hwang K. C., Choi Y. K., Jeong Y. I., Park K. B., Choi E. J., Jeong Y. W., Hossein M. S., Hyun S. H., Jeung E.-B., Hwang W. S.

Journal of Physiology and Pharmacology

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2020

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tom 71

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nr 3

7

Novobiocin sensitivity of Salmonella typhimurium dam and/or seqA mutants

75%

Chatti A., Aloui M., Tagourti J., Mihoub M., Landoulsi A.

Polish Journal of Microbiology

|

2014

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tom 63

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nr 1

This study was carried out to determine the effects of novobiocin, a gyrase inhibitor, on the growth, survival, motility and whole cell proteins of S. Typhimurium dam and/or seqA strains. Our results showed that the dam and seqA/dam mutants are the most sensitive to novobiocin, compared to wild type and seqA strains. Surprisingly, the motility of seqA mutants increased after exposure to novobiocin only in stationary phase cells. All the other strains showed a significant decrease in their motility. The analysis of protein profiles of all strains demonstrated several modifications as manifested by the alteration of the expression levels of certain bands. Our work is therefore of great interest in understanding the effects of novobiocin on S. Typhimurium and the involvement of DNA methylation.

8

Transcription factors and epigenetic modulation: its therapeutic implication in chronic kidney disease

75%

Hayasi K., Itoh H.

Archivum Immunologiae et Therapiae Experimentalis

|

2015

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tom 63

|

nr 3

9

Estimation of global content of 5-methylcytosine in DNA during allantoic and pulmonary respiration in chicken embryo

75%

Gryzinska M., Andraszek K., Jezewska-Witkowska G.

Folia Biologica

|

2014

|

tom 62

|

nr 2

10

DNA methylation dynamics under drought stress in barley

75%

Chwialkowska K., Szarejko I., Kwasniewski M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2013

|

tom 94

|

nr 3

11

Changes in DNA methylation in maize under herbicide stress conditions

75%

Tyczewska A., Gracz J., Szymkowiak J., Twardowski T.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

12

Mammalian DNA methyltransferases

75%

Siedlecki P., Zielenkiewicz P.

Acta Biochimica Polonica

|

2006

|

tom 53

|

nr 2

DNA methylation is an epigenetic process affecting gene expression and chromatin organization. It can heritably silence or activate transcription of genes without any change in their nucleotide sequences, and for a long time was not recognized as an important regulatory mechanism. However, during the recent years it has been shown that improper methylation, especially hypermethylation of promoter regions, is observed in nearly all steps of tumorigenesis. Aberrant methylation is also the cause of several major pathologies including developmental disorders involving chromosome instabilities and mental retardation. A great progress has been made in our understanding of the enzymatic machinery involved in establishing and maintaining methylation patterns. This allowed for the development of new diagnostic tools and epigenetic treatment therapies. The new approaches hold a great potential; several inhibitors of DNA methyltransferases have already shown very promising therapeutic effects.

13

Unintended consequences of plant transformation: a molecular insight

75%

Filipecki M., Malepszy S.

Journal of Applied Genetics

|

2006

|

tom 47

|

nr 4

Plant genomes are dynamic structures having both the system to maintain and accurately reproduce the information encoded therein and the ability to accept more or less random changes, which is one of the foundations of evolution. Crop improvement and various uncontrolled stress factors can induce unintended genetic and epigenetic variations. In this review it is attempted to summarize factors causing such changes and the molecular nature of these variations in transgenic plants. Unintended effects in transgenic plants can be divided into three main groups: first, pleiotropic effects of integrated DNA on the host plant genome; second, the influence of the integration site and transgene architecture on transgene expression level and stability; and third, the effect of various stresses related to tissue handling, regeneration and clonal propagation. Many of these factors are recently being redefined due to new researches, which apply modern highly sensitive analytical techniques and sequenced model organisms. The ability to inspect large portions of genomes clearly shows that tissue culture contributes to a vast majority of observed genetic and epigenetic changes. Nevertheless, monitoring of thousands transcripts, proteins and metabolites reveals that unintended variation most often falls in the range of natural differences between landraces or varieties. We expect that an increasing amount of evidence on many important crop species will support these observations in the nearest future.

14

Effect of DNA methylation on transient expression of PR-s genes in tobacco protoplasts

75%

Brodzik R., Hennig J.

Journal of Applied Genetics

|

1996

|

tom 37A

15

Igf2-H19, an imprinted tandem gene, is an important regulator of embryonic development, a guardian of proliferation of adult pluripotent stem cells, a regulator of longevity, and a ‘passkey’ to cancerogenesis

63%

Ratajczak M. Z.

Folia Histochemica et Cytobiologica

|

2012

|

tom 50

|

nr 2

16

Effects of resveratrol on the expression and DNA methylation of cytokine genes in diabetic rat aortas

63%

Lou X.-D., Wang H.-D., Xia S.-J., Skog S., Sun J.

Archivum Immunologiae et Therapiae Experimentalis

|

2014

|

tom 62

|

nr 4

17

Epi-genes potentiate plant biodiversity

63%

Szopa J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

18

Brachypodium distachyon – a model plant to study grass genome structure, dynamics and evolution

63%

Idziak D., Hasterok R., Betekhtin A., Borowska-Zuchowska N., Braszewska-Zalewska A., Chwialkowska K., Gorkiewicz R., Kus A., Kwasniewska J., Kwasniewski M., Robaszkiewicz E., Siwinska D., Wolny E., Chrominski K., Tkacz M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

19

Molecular mechanisms of lead toxicity

63%

Szymanski M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2014

|

tom 95

|

nr 2

20

DNA methylation, induced by beta-carotene and arachidonic acid, plays a regulatory role in the pro-angiogenic VEGF-receptor (KDR) gene expression in endothelial cells

63%

Kiec-Wilk B., Razny U., Mathers J. C., Dembinska-Kiec A.

Journal of Physiology and Pharmacology

|

2009

|

tom 60

|

nr 4

49-53

DNA methylation is a potent regulator of gene expression. The influence of beta-carotene (BC) and arachidonic acid (AA) on angiogenesis - a new blood vessel formation, was reported. The tyrosine kinase VEGFR-2 receptor (KDR) activation by vascular endothelial growth factor is one of the main angiogenic mechanisms. This study was aimed to investigate a possible role of CpG island methylation on regulation of the pro-angiogenic KDR gene expression after incubation of human endothelial cells with BC and/or AA. Methods: Human umbilical vein endothelial cells (HUVEC) were incubated with BC (1-10 µM) and/or 3 µM AA for 24 hours. The CpG island methylation was quantified using the COBRA method and restriction enzymes' digestion (NewEngland BioLabs). Intracellular protein concentrations were determined by Western blot analysis using the specific antibodies (Santa Cruz). Results: Incubation with BC and AA, decreased methylation of the KDR promoter region. These results well-correlated with the detected, by qRT-PCR, up-regulation of KDR gene expression by BC (p=0.035) as well as by AA. Incubation with BC (p=0.02) and AA (p=0.0014) increased the KDR protein levels in HUVECs. Conclusion: The changes in CpG island methylation of the KDR the pro-angiogenic gene promoter, represents one of the mechanisms involved in regulation of angiogenic response by BC and AA.

Ograniczanie wyników

Biochemical characteristics of the chromatin-remodeling enzyme DDM1

Role of genomic imprinting in endosperm development

Exogenous steroid hormones stimulate full development of autonomous endosperm in Arabidopsis thaliana

The role of DNA methylation in cancer development

Methylation pattern analysis of the sequences conding the 18S and 15SrRNA genes in the genomic DNA of wheat callus tissues

Demethylation of the sex-determining region Y gene promoter and incidence of disorder of sex development in cloned dog males

Novobiocin sensitivity of Salmonella typhimurium dam and/or seqA mutants

Transcription factors and epigenetic modulation: its therapeutic implication in chronic kidney disease

Estimation of global content of 5-methylcytosine in DNA during allantoic and pulmonary respiration in chicken embryo

DNA methylation dynamics under drought stress in barley

Changes in DNA methylation in maize under herbicide stress conditions

Mammalian DNA methyltransferases