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  1. 15 Journal of Plant Protection Research

  2. 6 Folia Biologica

  3. 3 Acta Biochimica Polonica

  4. 3 Cellular and Molecular Biology Letters

  5. 2 Advances in Agricultural Sciences

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  1. 16 Borowicz B. P.

  2. 2 Bednarek P. T.

  3. 2 Slota E.

  4. 1 Balcerkiewicz L.

  5. 1 Bieniek J.

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  1. 1 2016

  2. 1 2015

  3. 1 2013

  4. 1 2011

  5. 4 2010

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1

Separation of large DNA molecules on agarose gel. I. Classical (stable field) electrophoresis

100%
Wojcierowski J., Wojcierowski P.
Applied Biology Communications. Lublin Scientific Center
|
1991
|
tom 01
|
nr 6
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. IV. The product of the applied technologies

100%
Borowicz B. P.
Journal of Plant Protection Research
|
1997
|
tom 37
|
nr 1-2
The Polymerase Chain Reaction and other molecular technologies were applied to isolate the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene. This research object was realized and the importance of this result has been discussed in view of the mechanisms of the regulation of gene expression.
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. II. The technology for the preparation of the template

88%
Borowicz B. P.
Journal of Plant Protection Research
|
1997
|
tom 37
|
nr 1-2
The technology for the preparation of the template in the research aimed to isolate the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene is presented i.e. the linearisation of the template by Bam H I digestion, the purification of the template and the estimation of the template concentration.
4

Genomic DNA library of Veillonella parvula H2 in the rumen

75%
Liu G. W., Zhang Z. G., Yang W. Y., Yang L. Y., Wang Z.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 1
15-17
To construct a genomic library of Veillonella parvula H2, total DNA was extracted, sheared by a Hydroshear machine, and the DNA fragments ligated a into Smal-digested vector pUC18 before being transformed into E. coli DH5a. Colonies were selected on Luria-Bertani (LB) plates containing ampicillin, 5-bromo-4-chloro-3-indoyl-3-galactose (X-gal), and isopropy-β-D-thiogalactoside (IPTG) and proliferated. Recombinant plasmids were analysed for the presence of inserted DNA fragments of 3-4 kb by restriction mapping. The titre of the library was determined to be 10⁵ pfu/mL according to the formula N=ln(l-p)/(l-f). The genomic library consisted of 99% of the genome of Veillonella parvula, demonstrating a successful library construction.
5

Cross species amplification of coat colour genes in nutrias (Myocastor coypus Mol)

75%
Migdal L., Zabek T., Migdal A., Lapinski S., Bieniek J., Bugno-Poniewierska M.
Folia Biologica
|
2016
|
tom 64
|
nr 2
6

5S rRNA pseudogenes in a genome of callus culture of Lupinus angustifolius

75%
Karpinski S., Kedzierski W., Nuc P., Balcerkiewicz L., Pawelkiewicz J.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
1991
|
tom 39
|
nr 3
7

Noninvasive fluorescent screening of microinjected bovine embryos to predict transgene integration

75%
Rosochacki S. J., Kozikova L. V., Korwin-Kossakowski M., Matejczyk M., Poloszynowicz J., Duszewska A. M.
Folia Biologica
|
2003
|
tom 51
|
nr 1-2
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Cloning and expression of the two DNA fragments of the I-18 C region of Chironomus tentans. II. The effects of the applied technology

75%
Borowicz B. P.
Journal of Plant Protection Research
|
1998
|
tom 38
|
nr 1
The chromosomal I-18 C region of Chironomus tentans contains the I-18 C gene. Two different open reading frames (i.e. the ORF I and II) of two different transcripts (i.e. the 1.8 kb and 4.6 kb RNA) of the I-18 C gene of Chironomus tentans were isolated, at the DNA level, by the Polymerase Chain Reaction, then were cloned into the bluescript vector and finally were cloned into the pET-3a vector in order to translate them in T7 RNA polymerase / promoter system of E. coli. It was possible to obtain the ORF I overexpressed. In a case of the ORF II the low molecular weight polypeptide was detected, however it was not overexpressed, but still this polypeptide was strongly visible on the SDS-polyacrylamide gel.
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. II. Preparation of the intermediate vector - bluescript plasmid for ligation with the inserts

75%
Borowicz B. P.
Journal of Plant Protection Research
|
1999
|
tom 39
|
nr 1
The technology used for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments of the I-18 C gene in regard to the preparation of the intermediate vector i.e. the bluescript for the ligation with the ORF I and II reflecting DNA fragments, is presented. The main steps include the Eco RV digestion of the bluescript plasmid, the phenol / methylene chloride extraction of the plasmid, dephosphorylation of the bluescript plasmid and finally its extraction with the phenol and ethanol precipitation.
10
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. V. Different mechanisms of regulation regarding the open reading frames

75%
Borowicz B. P.
Journal of Plant Protection Research
|
1997
|
tom 37
|
nr 1-2
Different mechanisms of regulation regarding the Open Reading Frames (ORFs) have been discussed to have a broader perspective that is necessary to evaluate the role of the ORFs of the I-18 C gene. This consideration includes the ORF II of the I-18 C gene which is the object of the presented research.
11

The effect of DNA labeling with the fluorescent dyes R110 and R6G on genotype analysis using capillary electrophoresis

75%
Khan H. A.
Cellular and Molecular Biology Letters
|
2005
|
tom 10
|
nr 2
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. III. The DNA amplification technology

75%
Borowicz B. P.
Journal of Plant Protection Research
|
1997
|
tom 37
|
nr 1-2
The technology used to amplify and isolate the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene of Chironomus tentans is described i.e. the Polymerase Chain Reactioin and the agarose gel electrophoresis of this reaction product, as well as the blunting reaction of the product and its isolation.
13

Microdissected bovine X chromosome segment delineates homologous chromosomal regions in sheep and goat

63%
Bugno M., Zyga A., Slota E.
Folia Biologica
|
2008
|
tom 56
|
nr 3-4
149-151
14

Polymorphism of the genus Isophya [Orthoptera, Phaneropteridae, Barbitistinae] revealed by RAPD

63%
Grzywacz B., Warchalowska-Sliwa E.
Folia Biologica
|
2008
|
tom 56
|
nr 3-4
153-157
15
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Manipulation of vanillin synthesis genes in flax

63%
Pastor M., Boba A., Zuk M., Szopa-Skorkowski J.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2015
|
tom 96
|
nr 1
16

Genetic variability in the Antarctic hairgrass Deschampsia antarctica Desv. from maritime Antarctic and Subantarctic sites

63%
Chwedorzewska K. J., Bednarek P. T.
Polish Journal of Ecology
|
2008
|
tom 56
|
nr 2
209-216
The level of polymorphism, genetic variability and relatedness of the Antarctic hairgrass Deschampsia antarctica Desv. Populations from South Shetlands Is. (King George I., Penguin I., Livingstone I.), from vicinity of Antarctic Peninsula (Galindez I., Uruguay I.), and Falklands Is. (Jason Is., Sealion I.) were studied using the AFLP approach. Six EcoRI/MseI type selective primer pairs combinations were used for AFLP profiling and amplified scoreable DNA fragments; than AMOVA and PCA were performed. The level of molecular variability among all individuals from all the analysed populations was low and reach only 5.4 % irrespective of the distance between the localities and it was not significant at a broader geographical scale, even among the three groups of populations (South Shetlands Is., vicinity of Antarctic Peninsula, and Falklands Is.). PCA analysis shows that all populations formed uniform groups according to their locations.
17
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Cloning and expression of two DNA fregments of the I-18 C region of Chironomus tentans. I. The scheme of the performed experiments and the applied technology

63%
Borowicz B. P.
Journal of Plant Protection Research
|
1998
|
tom 38
|
nr 1
The I-18 C region of the polytenic chromosome of Chironomus tentans contains the I-18 C gene. Two DNA fragments, reflecting two different open reading frames (i.e.ORF I and II) of two different transcripts (i.e. 1.8 and 4.6 kb RNA) of the I-18 C gene were isolated, then were cloned into the intermediate vector and next to the final vector in order to translate them in T7 RNA polymerase / promoter system. The translation of the ORF I and II was performed to obtain the polypeptides of these ORFs for further study. Here, the scheme of the performed experiments and the applied technology that were necessary to realize the goal of this research, are presented.
18
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Cloning of the DNA fragments reflecting the open reading frame I and II of the I-18 C gene of Chironomus tentans. III. Preparation of the inserts for ligation with the bluescript and the modification of the vector

63%
Borowicz B. P.
Journal of Plant Protection Research
|
1999
|
tom 39
|
nr 1
The technology applied for the cloning of the open reading frame (ORF) I and II reflecting DNA fragments of the I-18 C gene of Chironomus tentans in regard to the preparation of the inserts for ligation with the bluescript and the modification of this intermediate vector, is described. The ORF I reflecting DNA fragment prepared for ligation with the bluescript vector, had one end blunt (i.e. the 5’ end with the Nde I restriction site) and the second end was sticky (i.e. the 3’ end after the Bam HI digestion of this insert). Subsequently, the bluescript vector for this ligation had also one end blunt (i.e. the 5’ end with the Nde I restriction site) and the second end was sticky (i.e. the 3’ end after the Bam HI digestion). The ORF II reflecting DNA fragment prepared for the ligation had both ends blunt and so the vector.
19
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. I. The technology for the preparation of the primers

63%
Borowicz B. P.
Journal of Plant Protection Research
|
1997
|
tom 37
|
nr 1-2
The above mentioned technology in a case of the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene is described i.e. the primers design, purification and phosphorylation of the oligonucleotide primers.
20

Evaluation of three methods for DNA fingerprinting of Corynebacterium pseudotuberculosis strains isolated from goats in Poland

63%
Stefanska I., Rzewuska M., Binek M.
Polish Journal of Microbiology
|
2008
|
tom 57
|
nr 2
Phenotypic approaches based on metabolic and biological characteristics of Corynebacterium pseudotuberculosis have been limited due to insufficient discrimination between closely related isolates. In this paper we present performance and convenience of three molecular typing methods: BOX-PCR, random amplification of polymorphic DNA (RAPD) and amplification of DNA fragments surrounding rare restriction site (ADSRRS-fingerprinting) in genome analysis of these bacteria. Among examined 61 strains there were distinguished four, eight and 10 different genotypes by BOX-PCR, RAPD and ADSRRS-fingerprinting, respectively. The value of discrimination index was the lowest for BOX-PCR (D = 0.265), much bigger for RAPD (D = 0.539) and the highest for ADSRRS-fingerprinting (D = 0.604). The good discriminatory ability and reproducibility of RAPD and ADSRRS-fingerprinting indicates that those techniques may be particularly applied for epidemiological studies of C. pseudotuberculosis isolates. We found that ADSRRS-fingerprinting is a rapid method offering good discrimination power, excellent reproducibility and may be applied for epidemiological studies of intraspecific genetic relatedness of C. pseudotuberculosis strains.
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