Wyniki wyszukiwania - AGRO

1

Mechanisms of DNA damage and repair, studied during the cell cycle

100%

Rzeszowska J.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

2

Activation and inhibition of ATM by phytochemicals: awakening and sleeping the guardian angel naturally

88%

Farooqi A. A., Wu S.-J., Chang Y.-T., Tang J.-Y., Li K.-T., Ismail M., Liaw C.-C., Li R.-N., Chang H. W.

Archivum Immunologiae et Therapiae Experimentalis

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2015

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tom 63

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nr 5

3

An evaluation of post-photodynamic therapy DNA damage in malignant cells using the comet assay

88%

Chwilkowska A., Saczko J., Drag-Zalesinska M., Wysocka T., Marcinkowska A., Malarska A., Banas T.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

4

DNA damage induced by hydrogen peroxide treatment at 4 st.C and 37 st.C in murine lymphoma L5178Y sublines

88%

Kruszewski M., Szumiel I.

Acta Biochimica Polonica

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1994

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tom 41

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nr 2

5

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

In memoriam: Professor Marcello Spano (1954-2017)

75%

Bonde J. P., Giwercman A.

Roczniki Państwowego Zakładu Higieny

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2017

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tom 68

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nr 2

6

Differential effects of heterocyclic amines on poly[ADP-ribose] polymerase-1 and mono-ADP-ribosyltransferase A

75%

Banasik M., Stedeford T., Strosznajder R. P., Hsu C.-H., Tanaka S., Ueda K.

Journal of Physiology and Pharmacology. Supplement

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2006

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tom 57

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nr 4

15-22

Heterocyclic amines (HCAs) have been shown to be carcinogenic in a variety of experimental systems. The purpose of the present study was to determine the in vitro effect of HCAs on the activity of the DNA repair enzyme poly(ADP-ribose) polymerase-1 (PARP-1). HCAs were also tested on the arginine-specific mono-ADP-ribosyltransferase A (MART-A), an enzyme involved in signal transduction and cytoskeletal realignment. 3-Amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) at 1 mM caused a 134% increase in PARP-1 activity and a 93% decrease in activity at 5 mM (IC50 = 2.2 mM). This dual effect is unique among inhibitors of this enzyme. On the other hand, Trp-P-2 activated MART-A at all concentrations tested, the peak being at 3 mM (>171% increase). In contrast, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) inhibited concentration-dependently both enzymes, PARP-1 (IC50 = 0.22 mM) and MART-A (IC50 = 2.8 mM). With nine other HCAs tested, predominantly inhibitory effects were observed. These results may assist our understanding of the carcinogenic mechanism of action and the dose-dependency of HCAs in animal bioassays.

7

5',8-cyclo-2'-deoxyadenosine [cdA] formation by gamma-radiation. Theoretical quantum mechanics study

75%

Karwowski B. T., Grand A., Cadet J.

Acta Biochimica Polonica

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2009

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tom 56

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nr 4

655-662

Reactions of reactive oxygen species and more specifically - of hydroxyl radical (•OH) - with nucleosides may lead to the generation of radicals in the base and 2-deoxyribose moieties. In the present study emphasis was put on the possible reaction modes of 2'-deoxyadenosine (dA) radicals, leading to the formation of related 5',8-cyclonucleosides. It appears that the prerequisite for the formation of 5',8-cyclo-2'-deoxyadenosine (cdA) is the adoption of O4'-exo conformation by 2-deoxyribose; however, this is the least energetically favored conformer among the different puckered forms adopted by the furanose ring. The O4'-exo conformation was found to be present in each of the discussed mechanisms.

8

Sperm epigenetic profile and risk of cancer

75%

Wdowiak A.

Journal of Pre-Clinical and Clinical Research

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2014

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tom 08

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nr 2

Introduction and objective. The integrity, stability and composition of sperm chromatin are of great importance in the fertilizing potential of male gametes and their capacity to support normal embryonic development. In this study, the author presents the current state of knowledge about the sperm epigenetic profile and risk of cancer. Abbreviated description of the state of knowledge. The obtaining of pregnancy and the state of health of the baby depends on the quality of the genetic material of both the female and the male. Health behaviours and environmental factors directly affect the quality of sperm, as well as the human egg cell and, consequently, on the reproductive capabilities, the course of pregnancy and the state of the newborn. There exist two thoroughly investigated epigenetic modifications: DNA methylation and histone modifications. The process of DNA methylation can be also a fundamental factor contributing to the development of cancer, where epigenotype undergoes significant modifications. When considering numerous DNA aberrations in the male gamete, the most commonly encountered is DNA fragmentation, particularly in infertile subjects. Surprisingly, an intracytoplasmatic sperm injection study of mice oocytes, using spermatozoa with a high DNA Fragmentation Index (DFI), revealed that a considerable percentage of adults born as a result of this method, showed a significant increase in the incidence of abnormal behavioural tests, malformations, cancer and signs of premature aging. Summary. The issue of assisted procreation raises the need to look for an appropriate treatment for males with sperm chromatin abnormalities. As a result, the fight against smoking addiction becomes the obvious necessity. Moreover, the reasonable solution nowadays seems to be supplementation with micronutrients and folic acid. It has been proved that the process of DNA fragmentation is a phenomenon that intensifies over time. Therefore, there should be a pursuance for, as close as possible, to the moment of ejaculation, application of semen to reproductive techniques. Finally, epigenetic changes are suspected of being one of the factors responsible for the deterioration of male sperm parameters observed in recent decades.

9

Is concentration and motility of male gametes related to DNA damage measured by comet assay?

75%

Dobrzynska M. M., Tyrkiel E. J., Derezinska E., Ludwicki J. K.

Annals of Agricultural and Environmental Medicine

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2010

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tom 17

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nr 1

73-77

10

Gamma radiation tolerance and protein carbonylation caused by irradiation of resting cysts in the free-living ciliated protist Colpoda cucullus

75%

Saito R., Koizumi R., Sakai T., Shimizu T., Ono T., Sogame Y.

Acta Protozoologica

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2020

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tom 59

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nr 2

11

75%

Widlak P.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 2

17

Formation of DNA crosslinks in human lymphocytes by acetaldehyde revealed by the comet assay

75%

Blasiak J., Gloc-Fudala E., Trzeciak A.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 2

Ethyl alcohol can be mutagenic, cancerogenic and teratogenic in man and its mutagenicity can be attributed to its first and major metabolite, acetaldehyde, which was reported to form adducts with DNA and proteins and DNA strand breaks. It was suggested that DNA crosslinks can be predominant DNA adducts of acetaldehyde but these results were obtained with the alkaline elution technique which provides no information on the extent of DNA damage at individual cell level. The comet assay is a technique, which allows detecting double- and single-strand DNA breaks caused by a broad spectrum of mutagens. It is also a suitable tool for the detection of crosslinking agents. Human peripheral blood lymphocytes were incubated for 1 h at 37°C with 3, 10, 30 and 100 mM of acetaldehyde. The alkaline comet assay was used to assess DNA damage. A dose-dependent decrease in the migration of DNA of acetaldehyde-treated cells was observed. Similar results were obtained when a recognized DNA crosslinking agent, formaldehyde was used. The results obtained suggest that acetaldehyde may form crosslinks with DNA in human lymphocytes. The nature of the crosslinks remains unknown and needs further investigations.

18

High levels of bulky DNA adducts in human sperm correlate with impaired fertility

75%

Horak S., Polanska J., Widlak P.

Acta Biochimica Polonica

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2003

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tom 50

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nr 1

Progressive decline in fertility and sperm quality has been reported over the last few decades, especially in industrialized nations. It has been proposed that exposure to factors that induce damage in DNA of spermatogenic cells may significantly contribute to impaired fertility. Here, the 32P-postlabelling method was used to analyze the levels of bulky DNA adducts in sperm cells in a group of 179 volunteers, either healthy subjects or patients with an impaired fertility. The levels of DNA adducts were 1.35-fold higher in the infertile group as compared to healthy individuals (P = 0.012). Similarly, a significant negative correlation between the levels of DNA adducts and measures of semen quality (sperm concentration and motility) has been observed (P < 0.001). In addition, the levels of bulky DNA adducts in sperm cells positively correlates with amounts of leukocytes in semen, which were significantly higher in semen of infertile subjects.

19

DNA damage caused by lipid peroxidation products

75%

Luczaj W., Skrzydlewska E.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 2

Lipid peroxidation is a process involving the oxidation of polyunsaturated fatty acids (PUFAs), which are basic components of biological membranes. Reactive electrophilic compounds are formed during lipid peroxidation, mainly α,β-unsaturated aldehydes. These compounds yield a number of adducts with DNA. Among them, propeno and substituted propano adducts of deoxyguanosine with malondialdehyde (MDA), acrolein, crotonaldehyde and etheno adducts, resulting from the reactions of DNA bases with epoxy aldehydes, are a very important group of adducts. The epoxy aldehydes are more reactive towards DNA than the parent unsaturated aldehydes. The compounds resulting from lipid peroxidation mostly react with DNA showing both genotoxic and mutagenic action; among them, 4-hydroxynonenal is the most genotoxic, while MDA is the most mutagenic. DNA damage caused by the adducts of lipid peroxidation products with DNA can be removed by the repairing action of glycosylases. The formed adducts have been hitherto analyzed using the IPPA (Imunopurification-32P-postlabelling assay) method and via gas chromatography/electron capture negtive chemical ionization/mass spectrometry (GC/EC NCI/MS). A combination of liquid chromatography with electrospray tandem mass spectrometry (LC/ES-MSMS) with labelled inner standard has mainly been used in recent years.

20

Nickel impairs the repair of UV - and MNNG-damaged DNA

75%

Wozniak K., Blasiak J.

Cellular and Molecular Biology Letters

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2004

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tom 09

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nr 1

Nickel(II) is reported to be genotoxic, but the mechanisms underlying its genotoxicity are largely unknown. It can interfere with DNA repair and this may contribute to its genotoxicity. We studied the effect of nickel chloride on the repair of DNA damaged by UV radiation or N-methyl-N-nitro-N-nitrosoguanidine (MNNG) in human lymphocytes using the alkaline comet assay. Nickel(II) at 1 μM caused an accumulation of DNA breaks during repair incubation, which could follow from the inhibition of the polymerization/ligation step of UV-damaged DNA repair. On the other hand, nickel(II) inhibited the formation of transient DNA breaks brought by the repair process after incubation with MNNG at 5 μM, which might follow from interference with the recognition/incision step of excision repair. Additionally, nickel at 1 μM inhibited the activity of formamidopyrimidine-DNA glycosylase (Fpg) and 3-methyladenine-DNA glycosylase II (Alk A), enzymes involved in DNA excision repair. A decrease in endonuclease III (Endo III) activity was observed at 2 and 5 μM of nickel chloride. Our results suggest that nickel(II) at non-cytotoxic concentrations can inhibit various steps of DNA excision repair, and this may contribute to its genotoxicity.

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