Wyniki wyszukiwania

1

Studies on the influence of aneuploidy of spermatozoa obtained from patients with oligozoospermia on their structure

100%

Stecewicz D., Warchol J. B.

Folia Histochemica et Cytobiologica

|

2001

|

tom 39

|

nr 1

2

Immunohistochemical assay of p53, cyclin D1, c-erbB2, EGFr and Ki-67 proteins in HPV-positive and HPV-negative cervical cancers

86%

Kedzia W., Schmidt M., Frankowski A., Spaczynski M.

Folia Histochemica et Cytobiologica

|

2002

|

tom 40

|

nr 1

3

Cryopreservation of plant cell cultures

86%

Van Iren F., Schrijnemakers E. W. M., Reinhoud P. J., Kijne J. W.

Biotechnologia

|

1996

|

nr 1

4

Flow cytometric determination of DNA content in some Sphagnum species of Subsecunda section

86%

Sliwinska E., Krzakowa M., Melosik I.

Biological Bulletin of Poznań

|

2001

|

tom 38

|

nr 2

5

Differential responses to neutral red uptake of mating type I and mating type II cells in Paramecium primaurelia. II. Change in the macronuclear DNA content

86%

Delmonte Corrado M. U., Ramoino P., Margallo E.

Acta Protozoologica

|

1992

|

tom 31

|

nr 1

6

Flow cytometric analysis of spermatogenesis in triploid Rana esculenta

86%

Brychta B. H., Tunner H. G.

Zoologica Poloniae

|

1994

|

tom 39

|

nr 3-4

7

Changes in DNA content during Lathyrus tuberosus L. endosperm and embryo development

86%

Knake-Sobkowicz S., Marciniak K.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2000

|

tom 42

|

nr 1

8

Nuclear DNA content of Solanum species grown in vitro, as determined by flow cytometry

86%

Maciejewska U., Skierski J. S., Szczerbakowa A.

Acta Physiologiae Plantarum

|

1999

|

tom 21

|

nr 1

The DNA relative content in nuclei from several Solanum species, which were used as partners for somatic hybridization, were determined using a flow cytometry method. The nuclei were isolated mechanically or via protoplasts from leaves of in vitro grown plants. In the case of S. nigrum as well as S. tuberosum cv. Bzura and dihaploid clone H8105, the nuclei were also obtained from suspension cultured cells by lysis of protoplasts. The source and the method of nuclei isolation affected the pattern of nuclear DNA in the genotypes studied. The mesophyll nuclei showed two distinct peaks on the DNA histograms, whereas the DNA peaks produced by cell suspension nuclei were broad and less distinct. The DNA content in the nuclei, calculated from the DNA histograms of the samples and a DNA standard historgam (Trout Red Blood Cells, having DNA content of 5.05 pg per nucleus), were much lower in mesophyll nuclei than in those obtained from the cell suspension for the same genotypes. The results are discussed in respect of the genetic instability of Solanum genotypes. The usefulness of a flow cytometry approach in somatic hybridization research is also discussed.

9

cAMP - a secondary messenger in higher plants. The effects of cAMP level on endoreduplication and cell size growth during root cell differentiation

86%

Aniol P., Godlewski M., Domanska A., Lbik-Nowak A., Bilecka A.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

10

Role of P53 tumor suppressor in ageing: regulation of transient cell cycle arrest and terminal senescence

86%

Wegierska-Gadek J., Wojciechowski J., Ranftler C., Schmid G.

Journal of Physiology and Pharmacology

|

2005

|

tom 56

|

nr 1

In this study we investigated the function of p53 as a regulator of cell cycle progression in cycling and senescent cells. Using the conditional temperature-sensitive (ts) mutant we could prevent the detrimental effect of constitutive expression of high levels of wt p53 protein. High levels of wt p53 inhibited cell proliferation by blocking the cells to progress from G1 to S phase of the cell cycle. Flow cytometric analysis revelaed a maintenance of G1 cell population for a longer time depending on the prolonged expression of wt p53 protein. The p53 mediated inhibition of cell proliferation and of the cycle was reversible. However, a spontaneous increase of wt p53 occurring in ageing normal human MRC-5 fibroblasts was associated with irreversible reduction of proliferative potential. The accumulation of G1 cells was detected by flow cytometry. By the measurement of DNA content it is not possible to discriminate between cells arrested in G1 and Go phase, therefore, the expression of G1 markers was determined. Analysis of the expression of distinct cell cycle regulators revealed that quiescent MRC-5 cells were in Go phase. Our results indicate that cell cycle arrest occurring in senescent cells is associated with the Go transition.

11

Haploid embryogenesis in capsaicinoid forms of soft-flesh Capsicum spp.

72%

Nowaczyk P., Nowaczyk L.

Herba Polonica

|

2009

|

tom 55

|

nr 3

251-256

The investigated material involved three pungent soft-flesh forms selected from a Capsicum frutescens L. x C. annuum L. hybrid. Capsaicinoid contents, determined by the HPLC technique in the fruit pericarp, amounted to 100 mg. kg-1. The anthers were cultured in vitro employing the method described by Chambonnet (1988) for C. annuum L. with modification involving an increase in kinetin content in R1 medium to 0.2 mg. dm-3. The efficacy of embryogenesis ranged between 0.25% and 4.12%, related to the ratio of the number of obtained plants to the number of cultured anthers, depending on genotype and the employed medium. Conversion of the embryos into plants and the general efficacy of androgenesis were highest in one line. In all of them, cytometrically determined DNA content in cells of leaves in the obtained regenerates amounted 1C.

12

Thy28 partially prevents apoptosis induction following engagement of membrane immunoglobulin in WEHI-231 B lymphoma cells

72%

Toyota H., Jiang X. Z., Asakura H., Mizuguchi J.

Cellular and Molecular Biology Letters

|

2012

|

tom 17

|

nr 1

Thy28 protein is conserved among plants, bacteria, and mammalian cells. Nuclear Thy28 protein is substantially expressed in testis, liver, and immune cells such as lymphocytes. Lymphocyte apoptosis plays a crucial role in homeostasis and formation of a diverse lymphocyte repertoire. In this study, we examined whether Thy28 affects induction of apoptosis in WEHI-231 B lymphoma cells following engagement of membrane immunoglobulin (mIg). Once they were established, the Thy28-overexpressing WEHI-231 cells showed similar expression levels of IgM and class I major histocompatibility complex (MHC) molecule compared with controls. The Thy28-overexpressing cells were considerably resistant to loss of mitochondrial membrane potential (ΔΨm), caspase-3 activation, and increase in annexin-positive cells upon mIg engagement. These changes were concomitant with an increase in G1 phase associated with upregulation of p27Kip1. The anti-IgM-induced sustained activation of c-Jun N-terminal kinase (JNK), which was associated with late-phase hydrogen peroxide (H2O2) production, was partially reduced in the Thy28-expressing cells relative to controls. Taken together, the data suggest that in WEHI-231 B lymphoma cells, Thy28 regulates mIg-mediated apoptotic events through the JNK-H2O2 activation pathway, concomitant with an accumulation of cells in G1 phase associated with upregulation of p27Kip1 in WEHI-231 B lymphoma cells.

13

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Effect of 2,4-dichlorophenoxyacetic acid pretreatment of Capsicum spp. donor plants on the anther culture efficiency of lines selected by capsaicinoid content

72%

Nowaczyk L., Nowaczyk P., Olszewska D., Niklas-Nowak A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 2

14

Various DNA content in myotube nuclei during myotomal myogenesis in Hymenochirus boettgeri [Anura: Pipidae]

72%

Daczewska M., Saczko J.

Folia Biologica

|

2003

|

tom 51

|

nr 3-4

15

Chromosome number and DNA content in taxa of Achillea L. in relation to the distribution of the genus

72%

Dabrowska J.

Acta Universitatis Wratislaviensis. Prace Botaniczne

|

1992

|

tom 49

16

Mitotic activity and changes in DNA content during Zea mays L. endosperm development

72%

Kuran H., Marciniak K.

Acta Societatis Botanicorum Poloniae

|

2002

|

tom 71

|

nr 3

17

Changes in mitotic activity and DNA content during the endosperm development of 3 Lathyrus species with different life cycle types

72%

Marciniak K., Knake-Sobkowicz S., Kuran H.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

18

Influence of extract from shoots of Taxus baccata var. 'Elegantissima' on mitotic activity of meristematic cells of Allium cepa L. roots

72%

Majewska A., Furmanowa M., Sliwinska E., Glowniak K., Guzewska J., Kuras M., Zobel A.

Acta Societatis Botanicorum Poloniae

|

2000

|

tom 69

|

nr 3

19

Polyploidization in the suspensor of Triglochin palustre l. [Juncaginaceae]

72%

Kozieradzka-Kiszkurno M., Swierczynska J., Bohdanowicz J.

Acta Biologica Cracoviensia. Series Botanica

|

2002

|

tom 44

Differentiation of the suspensor basal cell was studied in Triglochin palustre (2n = 24). The zygote divides into the smaller apical cell and the bigger basal cell, which becomes the basal cell of the suspensor. The nuclear DNA content of the suspensor basal cell attains a high degree of ploidy, up to 256C. Nuclei with the highest ploidy levels of 128C and 256C were observed in mature basal cells (from 50- to 500-celled embryos). As a result of polyploidization the volume of the nucleus increased and changes in the chromatin structure of polyploid nuclei were noted. Endochromocenters at middle ploidy level and bundle-like aggregations of chromatin at the highest ploidy levels were found. Rhythmic enlargement of the DNA content and nuclear volume of the basal cell, as well as the characteristic structure of its chromatin, point to endoreduplication as the mechanism of polyploidization in the suspensor.

20

Estrogen synthesis and DNA content in epidermal growth factor treated stromal cell cultures of human proliferative endometrium

72%

Tomaszewski J., Semczuk A., Kotarski J., Jakowicki J. A.

Folia Histochemica et Cytobiologica. Supplement

|

1997

|

tom 35

|

nr 2

Ograniczanie wyników

Studies on the influence of aneuploidy of spermatozoa obtained from patients with oligozoospermia on their structure

Immunohistochemical assay of p53, cyclin D1, c-erbB2, EGFr and Ki-67 proteins in HPV-positive and HPV-negative cervical cancers

Cryopreservation of plant cell cultures

Flow cytometric determination of DNA content in some Sphagnum species of Subsecunda section

Differential responses to neutral red uptake of mating type I and mating type II cells in Paramecium primaurelia. II. Change in the macronuclear DNA content

Flow cytometric analysis of spermatogenesis in triploid Rana esculenta

Changes in DNA content during Lathyrus tuberosus L. endosperm and embryo development

Nuclear DNA content of Solanum species grown in vitro, as determined by flow cytometry

cAMP - a secondary messenger in higher plants. The effects of cAMP level on endoreduplication and cell size growth during root cell differentiation

Role of P53 tumor suppressor in ageing: regulation of transient cell cycle arrest and terminal senescence

Haploid embryogenesis in capsaicinoid forms of soft-flesh Capsicum spp.

Thy28 partially prevents apoptosis induction following engagement of membrane immunoglobulin in WEHI-231 B lymphoma cells

Effect of 2,4-dichlorophenoxyacetic acid pretreatment of Capsicum spp. donor plants on the anther culture efficiency of lines selected by capsaicinoid content

Various DNA content in myotube nuclei during myotomal myogenesis in Hymenochirus boettgeri [Anura: Pipidae]

Chromosome number and DNA content in taxa of Achillea L. in relation to the distribution of the genus

Mitotic activity and changes in DNA content during Zea mays L. endosperm development

Changes in mitotic activity and DNA content during the endosperm development of 3 Lathyrus species with different life cycle types

Influence of extract from shoots of Taxus baccata var. 'Elegantissima' on mitotic activity of meristematic cells of Allium cepa L. roots

Polyploidization in the suspensor of Triglochin palustre l. [Juncaginaceae]

Estrogen synthesis and DNA content in epidermal growth factor treated stromal cell cultures of human proliferative endometrium