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DNA condensation characterised by fluorescence correlation spectroscopy [FCS]

100%

Hof M., Kral T., Langner M., Adjimatera N., Blagbrough I. S.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr Suppl.

2

Plasmid condensation induced by cationic compounds: hydrophilic polylysine and amphiphilic cationic lipid

86%

Chaszczewska-Markowska M., Ugorski M., Langner M.

Cellular and Molecular Biology Letters

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2004

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tom 09

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nr 1

The construction of an efficient carrier for genetic material is a major research objective that needs to be achieved before gene therapy can become a viable pharmacological approach. Artificial aggregates containing nucleic acids are one of the options for the systemic delivery of genetic information. The diversity of functions the aggregate is expected to fulfill necessitates its complex architecture. In order to obtain a complex supramolecular aggregate, formed from elements that are themselves complex molecules, appropriate procedures based on the detailed understanding of processes at the molecular level are required. In this study, we investigated how the various properties of cationic compounds affect nucleic acid condensation. The combination of two condensing agents, differing in their affinity towards water, when mixed with plasmids, resulted in aggregates which are resistant to enzymatic digestion and which form particles with well-defined size distributions. Such uniform and well-defined complexes may subsequently be further modified in order to obtain a fully functional genetic material carrier.

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Fluorescence correlation spectroscopy [FCS] as a tool to study DNA condensation with hexadecyltrimethylammonium bromide [HTAB]

86%

Kral T., Hof M., Jurkiewicz P., Langner M.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 2

The presented data show that the FCS technique can be used to detect the DNA condensation process induced with the cationic compound hexadecyltrimethylammonium bromide (HTAB). We have shown that HTAB induces plasmid condensation upon interaction with it. Condensation can be considered to be complete when the diffusion constant reaches its maximum. The HTAB induced increase in diffusion time does not correlate well with the changes observed when count rate and particle number are considered. This observation contradicts data published for another cationic agent, spermine. This apparent discrepancy proves that the mechanisms of interaction between these compounds and DNA are different. Consequently, the different characters of the plots of count rate, diffusion time, and particle number versus condensing agent concentration can be a source of additional information about the nature of cationic compound-DNA interaction.

Ograniczanie wyników

3 Cellular and Molecular Biology Letters

3 Langner M.

2 Hof M.

2 Kral T.

1 Adjimatera N.

1 Blagbrough I. S.

1 Chaszczewska-Markowska M.

1 Jurkiewicz P.

1 Ugorski M.

1 2005

1 2004

1 2002

DNA condensation characterised by fluorescence correlation spectroscopy [FCS]

Plasmid condensation induced by cationic compounds: hydrophilic polylysine and amphiphilic cationic lipid

Fluorescence correlation spectroscopy [FCS] as a tool to study DNA condensation with hexadecyltrimethylammonium bromide [HTAB]