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Divergence angles were measured for inflorescences of Helianthus annuus with several hundreds to more than a thousand disk flowers. Quantitative analysis showed that the angles are robustly fixed in the vicinity of the ideal golden angle 137.508° as accurately as ~0.001°. The mean deviation from the ideal value varies for each sample. The results have important implications for phyllotaxis models, which are discussed by referring to a necessary modification proposed by Roberts.
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Arbutin in Serratula quinquefolia M.B. [Asteraceae]

88%
Genus Serratula is known for the presence of steroid compounds which belong to the group of ecdysones. Former phytochemical works from the late sixties and early seventies indicate, in some Serratula species, the occurrence of a phenolic glycoside-arbutin. This has been confirmed in the present work through finding an α,β-arbutin anomer in Serratula quinquefolia M.B. New botanical data concerning the classification of genus Serratula suggest that the species in question should be regarded as belonging to genus Klasea (currently a section of genus Serratula). There has been an attempt to find a key to the chemical division within the taxons.
γ-Lactones are well known organic compounds. They can be isolated from many different plants, e.g. from Asteraceae (Compositae) family. They are used as active ingredients of various medicinal herbs and exhibit a variety of biological activities, i.e. antiviral, antibacterial, anticancer, antimalarial, anti-inflammatory and others. Among described compounds vernolide (7) is characterized by high antimicrobial activity; epoxy compounds like glaucolide (13) and hirsutinolide (24, 25, 26) possess high anticancer activity. Other noteworthy γ-lactone is vernopicrin (14) that shows antimalarial activity. Some of the γ-lactones possess interesting aromatic and flavon properties, which make them desirable ingredients in perfumery and food industry. Further studies to determine relationship between properties and complex composition are caused by diversity of structures and potential activities of γ-lactones. It can be used to design new analogues with interesting pharmacological, flavon and olfactory properties. This paper reviews selected studies concerning γ-lactones and their various biological activities.
The fraction of sterol acetates from the inflorescences of Hieracium pilosella has been isolated in the typical way from petroleum ether extract. By means of the weight method the total amount of sterols was determined (0.2659%). The mixtures of sterol acetates and free sterols were investigated using GC-MS techniques. The occurrence of about 18 sterols has been observed. Cholesterol, cholest-8(14)-en-3b-ol, cholesta-5.7-dien-3b-ol, cholest-7-en-3b-ol, ergosta-5.24-dien-3b-ol, campesterol, stigmasterol, b-sitosterol, fucosterol, 5a-stigmast-7-en-3a-ol were identified. The probable structures of lophenol, isofucosterol, 5a-stigmasta-7.24-dien-3b-ol, lanosta-9(11).24-dien-3b-ol and 24-ethylidene lophenol were stated on the basis of literature data. The last 4 sterols occur in a vestigial quantity, which made its identification impossible. Sitos erol and cholesterol are remarkably dominating sterols in the fraction.
The phagocyte-stimulatory properties of plants from Compositae family were studied. The extracts of Compositae family exhibit the stimulatory effect on the reticuloendothelial system. These properties depend on dose as well as the solubility of extract.
A comparative study of the sterol components of roots and herb of H. pilosella was carried out using GCMS techniques, β-sitosterol, stigmasterol, campesterol and α-saccharostenon were identified in both fractions. Stigmast-7-en-3-ol was identified only in herb and stigmast-4-en-3-on only in roots. Sitosterol and stigmasterol were the predominant sterol components in analysed raw materials. Besides, by means of the weight method the total amounts of sterols was determined: in herb 0.24% and in roots 0.16%.
Vernonia herbacea is a native species from the Brazilian Cerrado that accumulates about 80 % of inulin-type fructans in the underground reserve organs, the rhizophores. This work aimed at establishing a protocol for in vitro culture of V. herbacea, using seeds (achenes) and leaf discs as explants. Following germination and seedling growth, stem nodes from 6-month-old in vitro germinated plants were isolated and incubated on culture medium free of growth regulators for plant propagation and rhizophore formation. Fructan content and composition were evaluated in leaves, stems, roots and rhizophores from plants grown in vitro and compared with those of greenhouse-grown plants, in order to evaluate inulin production in vitro. Fructan contents of aerial organs and roots from in vitro plants were higher, compared with greenhouse plants, while in rhizophores, the opposite was observed. High performance anion exchange chromatography/pulsed amperometric detection profiles revealed the presence of the inulin homologous series in the aerial organs exclusively for in vitro plants, while in roots and rhizophores, this series was detected in plants grown in both conditions. These results indicate a modification in the source/sink ratio, leading to changes in the distribution of carbohydrates in in vitro plants. The leaf disc cultures on medium supplemented with indole-3-butyric acid induced the formation of roots (0.24, 0.49 µM) and friable callus (2.46 µM), while 6-benzylaminopurine (from 1.1 through 4.43 µM) induced compact callus. However, no shoot formation was observed. The use of seeds allowed the establishment of a protocol for in vitro culture and provides a model system for a better understanding of fructan metabolism in V. herbacea.
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