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Balea fallax (Rossm.) collected from the Roztocze Upland (SE. Poland) was kept in the laboratory for four years. Observations were conducted between March and October when the snails were kept at room temperature (18-25°C); in winter they were stored at 3°C. The egg-laying period started in late March and lasted till October, with maxima in spring and early autumn. The snails laid oval, gelatinous eggs with separatecalcium carbonate crystals in the external envelope (average egg size 1.96 × 1.73 mm). The eggs were deposited in batches (up to 14 eggs at a time) or singly. The number of batches per snail per year ranged between 1and 4. Snails isolated before maturation laid defective eggs which failed to develop, which suggest that the speciesis incapable of uniparental reproduction or at least the ability is very limited. The reproduction rate decreased during consecutive years, probably as a result of the aging or the shortage of allosperm in isolated individuals.Compared to batches of typically oviparous clausiliids, eggs of B. fallax hatched slightly earlier (interval between oviposition and hatching lasted 8-10 days at room temperature). It is likely that the adults retained developing eggs in the uterus for a short time. The juveniles needed at least 6 months to attain the ultimates hell size.
Vestia gulo and V. turgida are iteroparous simultaneous hermaphrodites. In the wild they are active from April till October; they reproduce in the spring and summer (egg retention from May to August), and hibernate from November till March. Their gonads show the greatest activity in the spring and summer (maturation of oocytes, intensive vitellogenesis: March–May, numerous mature oocytes: May–July; production and maturation of spermatozoa: March–May; numerous packets of mature spermatozoa: May–October), which coincides with the reproductive season. The onset of reproduction is determined by the size of the pool of vitellogenic and mature oocytes; oocyte production starts in the summer of the previous vegetation season and lasts till next spring. Mature spermatozoa are present in the gonad from spring till autumn which indicates an ability to mate during the whole active period. During hibernation the gonads contain no spermatids, mature spermatozoa or advanced vitellogenic oocytes.
Adults of Balea fallax sampled monthly from the Roztocze Upland were dissected to examine the occurrence of uterine egg retention. Developing eggs were found in snails collected between May and August. In June more than 60% of the studied individuals were gravid, while in other months egg-retaining snails constituted less than 10% of the sample. The number of retained eggs ranged from 3 to 17 (mean 12.3) and was not related to the adult shell size. In June the majority of eggs contained shelled embryos (maximum shell size1.1 whorl). The reproduction mode of B. fallax is compared with those of other ovoviviparous clausiliids of Central Europe.
The maturation, growth and reproduction of Alinda biplicata in the laboratory were studied over a five-year period. The snails were kept in pairs and groups of a few individuals. The initial material came from two populations from SW. Poland. The snails reproduced during the whole year, more intensively in the spring and autumn. We confirmed the ovoviviparity of A. biplicata: the whole embrynic development takes place in eggs retained in the parent's uterus. The snails gave birth to juveniles (no egg-laying was observed), and the uteri of dissected individuals contained from 3 to 15 eggs. The number of juveniles per litter was 1 to 8. The snails produced 3 to 20 juveniles in 2-9 batches per year. The neonate shells had 2.1-2.9 whorls and height of 1-1.6 mm. Growth to adulthood lasted 20 to 56 weeks and the time increased with increasing density of snails. The snails became sexually mature 5-6 months after completeion of shell growth and formation of the closing apparatus. The juvenile mortality ranged from 16.7% to 60.6% and increased with density. The life span in the laboratory was 220 to 295 weeks (4 to 6 years). These results are compared with those for other species; A. biplicata resembles other large ovoviviparous Clausiliidae species.
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