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The aim of this paper has been to examine experimentally the importance of the density of larvae and of the addition of the food for Chironomus and Tubificidae using selected parameters and indices of their populations. Increase of the density of Chironomus plumo- sus larvae (0.5–50.0 thousands ind. m⁻²) in laboratory experiments resulted in the decrease of emergence of imagos, number of tube apertures (3.5–0.4 apertures ind.⁻¹), and in the lower rate of tubes building. The addition of the food (powdered dry daphnids or food tablets for aquarial fish) had only slight effect on tube numbers but it decreased clearly the getting out of larvae from tubes (probably due to improved feeding conditions inside tubes). It had also a slight negative effect on the survival of larvae. Numbers and individual growth of Tubificidae were positively dependent on the addition of the food (also in the form of naturally dead Chironomus larvae) and negatively – on the density of Chironomus.
The time of Chironomus plu- mosus generation in the field conditions (shallow, eutrophic dam reservoir) was estimated to be about 3 weeks in the spring. This estimate has been possible due to simultaneous mass appearance of young larvae (the new generation) and the lack of older larvae at this time. Later in the season usually there was some amount of the youngest larvae, indicating the permanent emergence of imagos and the egg-laying, but without clear peaks of numbers and boundaries between successive generations. This regularity and the relatively low total numbers of larvae during the summer indicate the heavy fish pressure on the benthos, not allowing for the mass appearance of young larvae and the estimate of the generation time. Fish pressure is probably weak in spring, during a spawning period, but then increase in the summer. The generation number could be theoretically as high as 5 during the vegetation season (May–October), assuming about 3 weeks for full larval development, as it was estimated at optimal feeding and oxygen conditions and low fish pressure in the spring. However some limiting factors like: oxygen deficits, the annoyance by fish and bestrewing of larval tubes with the mud transported by the water flow (range 150–500 m³ s⁻¹ of the total inflow) increase in the summer. These factors can slow down larval development, resulting in observed lower generation number: 3 to 4 during a year.
During 9 years of studies very regular Chironomus population dynamics was stated in a eutrophic, lowland dam reservoir. There were usually two peaks of the abundance: the higher one at spring (up to 80 thousands ind. m⁻²) and the much lower in autumn. The duration of the spring Chironomus generation was about 3 weeks. The constant presence of young larvae during the summer did not result in the high total abundance of larvae, mainly due to the strong predation of fish and swallows on various stages of Chironomus. The smaller predators pressure in the spring (due to fish breeding) and in the autumn (due to lower temperatures) resulted in the mentioned two peaks. The spring peak abundance was positively correlated with the chlorophyll concentration in water (feeding resource for larvae) and negatively with the water flow. There was also negative correlation of the water flow and the chlorophyll concentration, as well as abundance of Chironomus and Tubificidae during the vegetation season (April-October). Tubificidae correlated strongly positively with the spring Chironomus numbers (with a month lag). The slight positive correlation of these benthic components abundance occurred for the whole vegetation season. Tubificidae occurred in generally high numbers up to 400 thousands m⁻², but various in different years, and with no regular changes during the season.
In looking for factors affecting Chironomus plumosus (L.) abundance and its fluctuations − the comparison of its numbers has been done in the bottom (at 6 m depth) and in “mesocosms” – trays with the same bottom sediment elevated 0.4-1.2 meter above the bottom. The experiment was carried out, in a shallow, strongly eutrophic, polymictic, lowland dam reservoir, where theabundance of Chironomus is among the highest in nature. The total abundance was on the average several times higher and more stable and larvae developed more quickly in trays over the bottom than in the same mud at the bottom. All this was probably due to better and more stable oxygen conditions above the bottom than at it’s very surface. Usually no differences in the abundance were found between levels 0.4, 0.8 and 1.2 m above the bottom, what indicates that conditions at all these levels were similar and different from those at the very bottom. The average density, and especially spring peak numbers in and above the bottom, were much higher in the year 1993 than in 1996 (the ratio of average numbers 1993/96 being similar – 2.3 and 2.6 for the bottom and trays respectively). It was probably due to a higher food supply (mostly small diatoms) by rivers and also due to weaker spring water flow in 1993. The very strong decline from high spring peak numbers to summer minimum seems to result from interconnections between larvae and perhaps also increased fish pressure, after the spawning period.
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