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1

Use of the real time xCelligence system for purposes of medical microbiology

100%
Junka A. F., Janczura A., Smutnicka D., Maczynska B., Secewicz A., Nowicka J., Bartoszewicz M., Gosciniak G.
Polish Journal of Microbiology
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2012
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tom 61
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nr 3
Roche’s xCelligence impedance-measuring instrument is one of a few commercially available systems of such type. According to the best knowledge of authors, instrument was tested so far only for eukaryotic cell research. The aim of this work was to estimate xCELLigence suitability for the microbiological tests, including (i) measurement of morphological changes in eukaryotic cells as a result of bacterial toxin activity, (ii) measurement of bacterial biofilm formation and (iii) impact of antiseptics on the biofilm structure. To test the influence of bacterial LT enterotoxin on eukaryotic cell lines, Chinese Hamster Ovary (CHO) cell line and reference strain Escherichia coli ATTC 35401 were used. To investigate Roche’s instrument ability to measure biofilm formation and impact of antiseptics on its development, Staphylococcus aureus ATTC6538 reference strain was used. The data generated during the experiments indicate excellent ability of xCelligence instrument to detect cytopathic effect caused by bacterial LT endotoxin and to detect staphylococcal biofilm formation. However, interpretation of the results obtained during real-time measurement of antiseptic’s bactericidal activity against staphylococcal biofilm, caused many difficulties. xCelligence instrument can be used for real-time monitoring of morphological changes in CHO cells treated with bacterial LT enterotoxin and for real-time measurement of staphylococcal biofilm formation in vitro. Further investigation is necessary to confirm suitability of system to analyze antiseptic’s antimicrobial activity against biofilm in vitro.
2

Nucleases isolated from Chelidonium majus L. milky sap can induce apoptosis in human cervical carcinoma HeLa cells but not in Chinese Hamster Ovary CHO cells

100%
Nawrot R., Wolun-Cholewa M., Gozdzicka-Jozefiak A.
Folia Histochemica et Cytobiologica
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2008
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tom 46
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nr 1
79-83
3
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Antioxidant and DNA repair stimulating effect of extracts from Leonurus sibiricus against an induced oxidative stress and DNA damage in CHO cells

80%
Sitarek P., Skala E., Wysokinska H., Wielanek M., Szemraj J., Sliwinski T.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2015
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tom 96
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nr 1
4

Expression of recombinant forms of human 21.5 kDa myelin basic protein and proteolipid protein in CHO cells

80%
Jaskiewicz E., Jedynak A., Ziolo E.
Acta Biochimica Polonica
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2005
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tom 52
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nr 4
MBP and PLP are major structural protein components of myelin. Both proteins play a functional role in formation of myelin sheath and in maintenance of its compaction. Immune responses to MBP and PLP have been implicated in the pathogenesis of multiple sclerosis (MS), an auto-immune disease of the central nervous system. Recombinant forms of both proteins isolated and purified from bacterial or insect cell systems are commonly used to study the specificity of auto-response in MS. We have prepared recombinant forms of MBP and PLP stably expressed in CHO cells. Several clones with proper cytoplasmic MBP or surface PLP localization were obtained and characterized by flow cytometry and indirect immunostaining. CHO cells expressing the recombinant forms of MBP and PLP can be very useful in studies on the autoimmune mechanism of MS.
5

Redox changes in normal and neoplastic cells during the cell cycle I. Bioreduction of nitroxides by CHO cells with different mitotic activity

80%
Panz T.
Current Topics in Biophysics
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1994
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tom 18
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nr 2
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