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Five novel SNPs of the bovine LHX4 gene and their association with growth traits in native Chinese cattle breeds

100%

Liu J. X., Ren G., Chen H., Li F., Lan X. Y., Li M. J., Wang J. Q.

Animal Science Papers and Reports

2011

tom 29

nr 1

LHX4 is a LIM homeodomain transcription factor involved in pituitary ontogenesis. Some mutations of LHX4 are associated with combined pituitary hormone deficiency that affects growth and development in animal. The objective of this study is revealing to these polymorphisms of in LHX4 gene exons 1, 2, 3 in 820 Chinese cattle and to assess possible association of polymorphisms in LHX4 gene with growth traits in NY breed. The PCR-SSCP and DNA sequencing of LHX4 gene showed five novel synonymous mutations: (GenBank: NW_001493442.2: g.35143G >A, g.35152C>T, g.35212C>T, g.35230G>A, g.35233T>C). Genotype MM (GG-CC-CC-GG-TT) and haplotype M (G-C-C-G-T) were dominant in the four breeds, and genotype frequencies of LHX4 in the four cattle populations agreed with Hardy-Weinberg equilibrium (P>0.05), although their frequencies significantly differed among the four analyzed populations (***P<0.001). The association analysis showed that individuals with genotype MM had greater body weight than those with genotype MN (*P<0.05) at eighteen months of age in Nanyang cattle.

Identification of BLAD and citrullinemia carriers in Chinese Holstein cattle

84%

Li J., Wang H., Zhang Y., Hou M., Zhong J., Zhang Y.

Animal Science Papers and Reports

2011

tom 29

nr 1

Bovine leukocyte adhesion deficiency (BLAD) and citrullinemia are two autosomal recessive genetic diseases in Holstein cattle and both result in death of homozygous animals. Through the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology and DNA sequencing, one citrullinemia and three BLAD carriers were found in a population of 615 Chinese Holstein cattle, including 436 cows and 179 bulls. Citrullinemia and BLAD carrier frequency was 0.16% and 0.49%, respectively, in tested Chinese Holstein cattle. In this study, the first citrullinemia carrier occurring in Chinese Holstein cattle is described, although the world frequency of this disorder is very low.

Karyotype vs DNA fingerprinting in geese

67%

Jaszczak K., Zawadzka M., Jaszczak J., Rabsztyn A.

Animal Science Papers and Reports

2002

tom 20

nr 2

Ograniczanie wyników

3 Animal Science Papers and Reports

1 Chen H.

1 Hou M.

1 Jaszczak J.

1 Jaszczak K.

1 Lan X. Y.

1 Li F.

1 Li J.

1 Li M. J.

1 Liu J. X.

1 Rabsztyn A.

1 Ren G.

1 Wang H.

1 Wang J. Q.

1 Zawadzka M.

1 Zhang Y.

1 Zhong J.

2 2011

1 2002

Wyniki wyszukiwania

Five novel SNPs of the bovine LHX4 gene and their association with growth traits in native Chinese cattle breeds

Identification of BLAD and citrullinemia carriers in Chinese Holstein cattle

Karyotype vs DNA fingerprinting in geese