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1

Wpływ chinozolu i formaliny na pałeczki Brucella i prątki gruźlicy w szczepionce pryszczycowej l. W.

100%
Decowski M., Dziubkiewicz H., Zorawski C.
Medycyna Weterynaryjna
|
1959
|
tom 15
|
nr 08
2

Nowoczesne aspekty diagnostyki molekularnej brucelozy

84%
Szulowski K., Murat J.
Medycyna Weterynaryjna
|
2008
|
tom 64
|
nr 06
741-744
The diagnostics of brucellosis is primarily based on serological investigations. Since a serologically positive response can occur from antigenically cross-reactive bacteria, the isolation and identification of the pathogen from a potentially infected host remains the gold standard as a tool for the confirmation of infection. However, this process also has drawbacks. The tests are time consuming, complex and must be performed by highly skilled personnel. The zoonotic nature of most Brucella species is a potential hazard for laboratory personnel who must manipulate the infectious agent during testing. Finally, some of the characteristics are subjective and the results are not always definitive. Recently, progress in these aspects of diagnostics has been advanced through PCR-based technologies. PCR-based assays are simple to design and carry out, rapid, inexpensive to perform and are characterized by high diagnostic values: sensitivity and specificity. The following is a review of the major assays constituting excellent progress in the diagnostics of brucellosis.
3

Evaluation of in vitro activities of tigecycline and various antibiotics against Brucella spp.

84%
Ozhak-Baysan B., Ongut G., Ogunc D., Gunseren F., Sepin-Ozen N., Ozturk F., Aktepe O. C., Gultekin M.
Polish Journal of Microbiology
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2010
|
tom 59
|
nr 1
55-60
Brucellosis is a zoonosis with a worldwide distribution and remains a significant public health problem mainly in the developing world. In this study we evaluated the in vitro activities and synergistic effects of antibiotic combinations against blood culture isolates of Brucella spp. In vitro susceptibilities of 76 blood culture isolates of Brucella melitensis and one blood culture isolate of Brucella abortus to doxycycline, streptomycin, gentamicin, trimethoprim-sulfamethoxazole, moxifloxacin, rifampin, ciprofloxacin, and tigecycline were examined by Etest method. For 37 patients with Brucella spp. isolates (36 B. melitensis, 1 B. abortus), antibiotic combinations used for treatment were identified with those tested in vitro for synergy using Etest method. Trimethoprim-sulfamethoxazole and tigecycline were the most active of the compounds tested with MIC₉₀ value of 0.094 mg/1. Among antibiotic combinations only streptomycin-rifampin combination was synergistic for one Brucella spp. isolate. The other antibiotic combinations revealed antagonistic or indifferent activity. Complete clinical response was achieved in all patients. Further studies are required to determine the correlation between the antimicrobial susceptibility and synergy test results with the clinical course of patients. Brucellosis can be adequately treated with existing regimens in our region.
4

A novel, rapid, and simple PMA-qPCR method for detection and counting of viable Brucella organisms

84%
Zhang S.-J., Wang L.-L., Lu S.-Y., Hu P., Li Y.-S., Zhang Y., Chang H.-Z., Zhai F.-F., Liu Z.-S., Li Z.-H., Ren H.-L.
Journal of Veterinary Research
|
2020
|
tom 64
|
nr 2
5

Comparative investigations of urease test and Brucella agar plating in the diagnosis of Helicobacter pylori infections

84%
Grodowska A., Dzieniszewski J., Jarosz M., Popowski J., Kafel S.
Journal of Physiology and Pharmacology. Supplement
|
1997
|
tom 48
|
nr 1
6

Bruceloza - choroba odzwierzeca

67%
Pilaszek J., Pilaszek M.
Magazyn Weterynaryjny
|
2006
|
tom 15
|
nr 11
44-47
7

Diagnosis of bovine brucellosis using traditional serological techniques and fluorescence polarisation assay

67%
Weiner M., Iwaniak W., Zlotnicka J., Szulowski K.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 4
The aim of this study was the application of fluorescence polarisation assay (FPA) for the examination of bovine sera and comparison of the results of the assay with the results of Rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT), and ELISA. Six hundred thirty-five sera from cattle, including 300 sera from healthy animals, 32 sera from animals regarded as serologically positive for brucellosis and culled, and 303 sera originated from confirmatory investigations were used. All sera originating from healthy animals, negative in ELISA, RBT, SAT, and CFT were also negative in FPA. Among 303 sera from confirmatory investigations, 269 were positive in both RBT and SAT, 21 were positive in SAT and remaining 13 were RBT-positive only. Only two sera, one positive in both tests (RBT, SAT) and one SAT-positive, were also positive in FPA. Among 32 sera originated from animals regarded as serologically positive, which reacted in RBT, SAT, and CFT, 14 gave positive results in ELISA, whereas 18 were negative. Among these ELISA-positive sera, 13 were also positive in FPA. All samples positive in SAT, RBT, and CFT, and negative in ELISA, were also negative in FPA.
8

Lizozym i jego charakterystyka. Wlasciwosci biologiczne i fizykochemiczne

67%
Trziszka T., Kopec W.
Przemysł Spożywczy
|
1997
|
tom 51
|
nr 01
41-43
W artykule opisano podstawowe właściwości biologiczne i fizykochemiczne lizozymu - białka enzymatycznego - N-acetylomuramylo β (1,4) glikanohydrolazy (EC. 3.2.1.17). Lizozym jest składnikiem szeregu tkanek oraz błon komórek roślinnych i zwierzęcych. Lizozym ma zdolność rozpuszczania błon komórkowych bakterii G+, a w określonych warunkach także G-. Szczególnie istotne jest hamowanie rozwoju bakterii patogennych takich jak: Salomonella, Brucella, Pseudomonas, Listeria monocytogenes oraz proteolityczne szczepy Clostridium botulinum. Lizozym tworzy kompleks z owomucyną, budujący żelową strukturę białka jaja. Lizozym jest termooporny i może być ogrzewany nawet do 100°C.
9

Comparison of different PCR methods for detection of Brucella spp. in human blood samples

67%
Al-Ajlan H., Ibrahim A., Al-Salamah A.
Polish Journal of Microbiology
|
2011
|
tom 60
|
nr 1
For detection of Brucella species by PCR four DNA extraction methods and four targets were compared using pure culture of Brucella melitensis and the best conditions were applied in clinical samples. It was found that the MagNA Pure LC method was the most efficient and sensitivemethod showing a positive PCR reaction with DNA extracted from as low as 25 and 100 CFU suspended in one ml blood and one ml water, respectively. Detection of Brucella spp. by conventional PCR was investigated using four different targets. The results indicated that The B4-B5 amplification method was the most sensitive one as it could amplify DNA extracted from as a low as 25 and 100 CFU/ml suspended in one ml water and blood, respectively. Furthermore real-time PCR was able to detect Brucella using DNA extracted from as low as 50 CFU/ml blood and 15 CFU/ml water, respectively. The best and optimum detection conditions were applied to the clinical samples. Evaluation of conventional PCR assays on blood specimens confirmed 72% of the results obtained by conventional blood culture methods with a specificity of 95%, while serum samples had a sensitivity of 54% and specificity of 100%. Real-time PCR was generally found to be more sensitive and specific for detecting Brucella spp. in blood and serum samples compared to conventional PCR. The real-time PCR done on blood specimens confirmed 77.5% of the results obtained by conventional blood culture methods with specificity of 100%, while 60% of serum samples were found to be positive with specificity of 100%. These results suggest that serum and blood analysis by conventional and real time PCR is a convenient and safe method for rapid and accurate diagnosis of brucellosis.
10

Detecting Toxoplasma, Listeria and Brucella antibodies in goitered gazelles in Turkey

67%
Gokcen A., Altas M. G., Sevgili M., Babur C., Celebi B., Kilic S.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 09
1064-1066
The aim of this study was to determine the prevalence of toxoplasmosis, listeriosis and brucellosis in goitered gazelles (Gazella subgutturosa) in Sanliurfa region, Turkey. A total of 82 sera were collected from healthy gazelles and tested for listeriosis, brucellosis and toxoplasmosis by the Osebold Agglutination Test (OAT), Serum Agglutination Test (SAT) and Sabin-Feldman Dye Test (SFDT), respectively. 82 gazelles 5 (6.09%) were seropositive for listeriosis, 23 (28.04 %) for toxoplasmosis and all of them were seronegative for brucellosis. No statistically significant differences were observed between male and female gazelles in the seroprevalences of toxoplasmosis and listeriosis. As a result, the presence of anti-Toxoplasma gondii and Listeria spp. specific antibodies in G. subgutturosa in the region of Sanliurfa was determined.
11
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Seroprevalence of Q fever, brucellosis and leptospirosis in farmers and agricultural workers in Bari, Southern Italy

67%
Monno R., Fumarola L., Trerotoli P., Cavone D., Giannelli G., Rizzo C., Ciceroni L., Musti M.
Annals of Agricultural and Environmental Medicine
|
2009
|
tom 16
|
nr 2
205-209
12

Zwierzeta wolno zyjace jako rezerwuar drobnoustrojow Brucella

67%
Szulowski K., Pilaszek J.
Medycyna Weterynaryjna
|
2000
|
tom 56
|
nr 11
687-690
13
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Brucellosis in humans - etiology, diagnostics, clinical forms

59%
Galinska E. M., Zagorski J.
Annals of Agricultural and Environmental Medicine
|
2013
|
tom 20
|
nr 2
Brucellosis in humans is a zoonosis of greatly varied clinical image. It occurs on all inhabited continents. The course of the disease may be acute, sub-acute or chronic. The etiologic factors of brucellosis are small, aerobic Gram-negative rods of the genus Brucella, which currently contains ten species: B. abortus, B. suis, B. ovis, B. melitensis, B. canis, B. neotomae, B. pinnipedialis, B. ceti, B. microti and B. inopinata. In humans, the disease is caused mainly by: B. melitensis as the most pathogenic species, followed by B. suis, whereas B. abortus is considered as the mildest type of brucellosis. The natural reservoir of the germ and the source of infection in humans are infected domestic animals, primarily cattle, sheep, goats, as well as wild animals. Infection in humans occurs by penetration through damaged skin, conjunctiva, and more rarely via the alimentary route by the consumption of infected products. Especially exposed are: veterinarians, veterinary technicians, insemination service employees, zoo technicians, farmers working on multi-herd farms (production cooperatives), e.g. cattlemen, also private farmers, employees of slaughter houses and meat processing enterprises. A basis for diagnosing brucellosis are serologic tests which allow the detection of antibodies occurring in response to infection, performed with the use of the following methods: agglutination test, complement fixation test, Coombs test, 2-mercaptoethanol agglutination test, and Burnet’s intradermal allergy test which detects the state of hypersensitivity of the infected organism to Brucella abortus rods.
14

Mozliwosc wystepowania bakterii chorobotworczych w mleku surowym

59%
Molska I., Zmarlicki S.
Higiena
|
2002
|
nr 1
15-19
15

Praktyczne zastosowanie pomiaru pH mleka surowego do oceny jego swiezosci

59%
Grega T.
Przegląd Mleczarski
|
1995
|
nr 06
167-170
16
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Charakterystyka zapaleń gruczołu mlekowego u krów wywoływanych przez odżywnościowe patogeny człowieka

51%
Malinowski E., Gajewski Z.
Życie Weterynaryjne
|
2009
|
tom 84
|
nr 04
17

Srodowisko zanieczyszczone patogenami zrodlem zakazenia dla trzody chlewnej

43%
Glinski Z., Kostro K.
Trzoda Chlewna
|
2005
|
tom 43
|
nr 07
102
18
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Choroby odzwierzęce i ich czynniki etiologiczne w raporcie Europejskiego Urzędu do spraw Bezpieczeństwa Żywności za 2008 r.

43%
Osek J., Wieczorek K.
Życie Weterynaryjne
|
2010
|
tom 85
|
nr 04
19
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Zoonozy pokarmowe i ich czynniki etiologiczne wg raportu EFSA za 2009 r.

43%
Osek J., Wieczorek K.
Życie Weterynaryjne
|
2011
|
tom 86
|
nr 08
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