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Cytophotometric analysis of DNA content in cell nuclei of five Bromus species of Genea section

100%
Naganowska B.
Genetica Polonica
|
1993
|
tom 34
|
nr 3
215-221
2
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Content available

Karyotypes of five Bromus species of Genea section

86%
Naganowska B.
Genetica Polonica
|
1993
|
tom 34
|
nr 3
197-213
3

Rodzaj Bromus (Poaceae) na terenach kolejowych Lubelszczyzny

72%
Wrzesien M.
Fragmenta Floristica et Geobotanica Polonica
|
2009
|
tom 16
|
nr 2
4

Embryo morphology in the genus Bromus [Poaceae]

72%
Kosina R.
Fragmenta Floristica et Geobotanica
|
1996
|
tom 41
|
nr 2
5

RAPD studies in Bromus (Poaceae) from the old and new worlds - preliminary results

72%
Joachimiak A., Sutkowska A., Mitka J.
Acta Biologica Cracoviensia. Series Botanica
|
2001
|
tom 43
RAPD analysis was applied to nine Bromus species (B. fasciculatus, B. arvensis, B. scoparius, B. hordeaceus, B. squarrosus, B. carinatus, B. willdenowii, B. erectus, B. inermis) belonging to four subgenera: subg. Stenobromus, subg. Bromus, subg. Ceratochloa, and subg. Festucaria. With 10 out 13 primers a considerable degree of polymorphism was detected at the interspecific level, and some bands obtained with these primers appeared to be species-specific. The RAPD band distribution and genetic relationships between different Bromus species and subgenera were analyzed. No bands common to all analyzed species were discovered, but some of the amplified DNA fragments were common to all taxa belonging to the same subgenera or even different ones. The divisions suggested by taxonomists generally were confirmed by numerical analysis of the RAPD data presented here.
6

C-banding and nuclear DNA amount in six Bromus species

72%
Joachimiak A., Kula A., Sliwinska E., Sobieszczanska A.
Acta Biologica Cracoviensia. Series Botanica
|
2001
|
tom 43
Conventional and C-banding chromosome studies and nuclear DN A estimations were performed on six Bromus species (B. arvensis, 2n = 2x = 14; B. hordeaceus, 2n = 4x = 28; B. carinatus, 2n = 8x = 56; B. willdenowii, 2n = 6x = 42; B. erectus, 2n = 8x = 56; and B. inermis, 2n = 8x = 56) belonging to three subgenera: subg. Bromus, subg. Ceratochloa and subg. Festucaria. Three species (B. carinatus, B. erectus and B. inermis) show different chromosome numbers within root-tip meristem. Root-tip cells of these high polyploid species (2n = 8x = 56) showed substantial DNA variability. Densitometric and flow-cytometric analyses clearly showed that many interphase nuclei are beyond the expected 2C-4C limit characteristic of cycling cells. The most distinct DNA instabilities were observed in B. carinatus, where we found especially high numerical chromosome variations and many chromosome numbers higher and lower than 2n = 8x = 56. Such quantitative DNA variability is substantially reduced beyond the meristematic part of the roots and virtually absent within leaf mesophyll cells. Estimated nuclear 2C DNA content was 11.63 pg in B. arvensis, 23.03 pg in B. hordeaceus, 22.94 pg in B. carinatus, 12.99 pg in B. willdenowii, 24.65 pg in B. erectus and 24.54 pg in B. inermis. By DNA amount, the analyzed taxa formed two distinct groups: with 2C DNA value at about 12 pg (B. arvensis and B. willdenowii), and at about 24 pg (B. hordeaceus, B. carinatus, B. erectus and B. inermis). Estimated DNA values were not linked with ploidy level. All species showed a similar, mainly telomeric heterochromatin distribution, but small amounts of intercalary and centromeric heterochromatin were also seen. The majority of analyzed species also had similar heterochromatin amounts within karyotype. The only exception was B. carinatus with 16.20% heterochromatin (calculated as percent of karyotype length). It is suggested that the observed 77% nuclear DNA difference between two closely related representatives of subg. Ceratochloa (B. carinatus and B. willdenowii) is to some extent a result of heterochromatin accumulation in B. carinatus chromosomes.
7

RAPD analysis points to old world Bromus species as ancestral to new world subgen. Festucaria

58%
Sutkowska A., Mitka J.
Acta Biologica Cracoviensia. Series Botanica
|
2008
|
tom 50
|
nr 2
117-125
The genus Bromus subgen. Festucaria is a widespread Old World and New World taxon having genomes A, B and L, distinguished cytogenetically. Stebbins (1981) suggested that evolution in Bromus went from the small genomes A and B to the large genome L. Thus, Old World species with genomes A and B could be ancestral to New World species with genome L. To test this hypothesis we carried out RAPD analysis of a representative group of species from subgen. Festucaria. RAPD band patterns enabled resolution of 13 species after excluding the species-specific bands with high/moderate support. The basal position of Bromus variegatus M. Bieb. - an Old World species presumably having a B genome - in relation to some New World species with the genome L confirmed Stebbins' hypothesis of its ancestry in relation to the Old World species. The group of high bootstrap support, B. cappadocicus Boiss. et Balansa-B. erectus Huds.-B. riparius Rehm., with genome A. and a distinctly xerothermic ecological profile, was related to a New World species with genome L, B. auleticus Trin. ex Nees, pointing to their presumably common evolutionary history.
8

Stoklosa Bromus

44%
Bonenberg K.
Aura
|
2004
|
nr 05 Ziola a Zdrowie nr 80
1-2
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