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The bio-efficacy of four plant leaf extracts viz., apple of sodom, Calotropis procera Aiton; Mexican poppy, Argemone mexicana Linnaeus; Mexican marigold, Tagetes minuta Linnaeus and Indian neem, Azadirachta indica were tested against mustard aphid, Lipaphis erysimi on Indian mustard, Brassica juncea. The highest per cent aphid reduction during first, second and third spray were 28.79, 40.52 and 59.32 at 1 : 10 g/ml; 34.70, 44.49 and 66.14 at 1 : 5 g/ml and 53.88, 64.84 and 100.00 at 1 : 2.5 g/ml with Indian neem. However, Mexican marigold was also effective at highest concentration (1 : 2.5 g/ml) and reduced 96.38 per cent L. erysimi. All the treatments of plant leaf extracts showed insecticidal activity, but Indian neem followed by Mexican marigold reduced the aphid population to a great extent.
Nitrate reductase activity in gibberellic acid and kinetin treated mustard (Brassica juncea Coss. cv. T-59 ‘Varuna’) seedlings, grown in the presence or absence of light and/or NO₃ was investigated. While both light and NO₃, alone could induce NR activity, their combination showed additive effects. Kinetin treatment significantly promoted both light- and NO₃- induced NR activities, assayed by either in vivo or in vitro techniques, whereas, gibberellic acid was almost ineffective. In the absence of both light and NO₃, however, phytohormones alone could not induce NR activity. Both light-induced and NO₃ induced NR fractions had a pH optima of 7.5, preferred NADH as an electron donor (NADH: NADPH ratio 2.5) and Km values for NO₃ was 0.2 mM. Actinomycin D, cycloheximide and tungstate were equally effective in suppressing the development of NR activity after exposure to light or NO₃. These results indicate that two independent NR fractions operate, with apparently identical properties but separate control mechanisms.
Seeds of four raya cvs., viz. RL 1359, RLM 198, RLM 619 and RLM 514 were desiccated for 2, 7,15 and 45 days wchich led to different levels of seed moisture. Data on germination capacity speed of germination, seedling dry weight, electrical conductance, dehydrogenase activity and seed storage behaviour for up to two years after desiccation were recorded. Based on this data, cvs. RL 1359 and RLM 514 were found to be tolerant to desiccation to as low as 0.73 and 1.16 per cent seed moisture levels, respectively. Seeds of RLM 198 tolerated desiccation up to 2.28 per cent seed moisture. However, RLM 619 seeds showed desiccation injuries in seedling dry weight, electrical conductance and speed of germination at moisture levels below 5.67 per cent.
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