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1

Symbiotic properties of Bradyrhizobium sp. [Lupinus] assayed on serradella plants

100%
Derylo M., Bednara J., Glowacka M., Skorupska A.
Acta Societatis Botanicorum Poloniae
|
1995
|
tom 64
|
nr 4
359-365
2
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Wplyw drobnoustrojow glebowych na rozwoj Rhizobium i Bradyrhizobium i ich symbioze z roslinami motylkowatymi. I. Wplyw autochtonicznej mikroflory glebowej i wolno zyjacych asymilatorow N2 ...

86%
Strzelec A.
Postępy Nauk Rolniczych
|
1995
|
tom 42
|
nr 2
25-35
The results of investigatiolls on the effect of soil microflora on Rhizobium and Bradyrhizobium strains development, their symbiosis activity with Legume plants and the effect of joined seeds inoculation with specific symbiotic bacteria and Azospirillum or/and Azotobacter strains are discussed. The results show the presellce of high number of antagonistic microorganisms to symbiotic bacteria, which could inhibit their growth, proliferation and survival in soil environment. This confirms the necessity of pre-sowing Legume plant seeds inoculation with specific symbiotic bacterial strains. Many investigators have observed the beneficial effect of joined Legume plant seeds inoculation with specific symbiotic bacteria and correctly selected Azotobacter or/and Azospirillum strains on symbiosis effectivity. The studies have shown that the effect of joined inoculation is influenced by plant species and cultivars, strain properties, the ratio of inoculant cell number in prepared inoculum, and the way and term of inoculum application. The studies leading to the explanation of mechanisms of the Azospirillum and Azotobacter influence on symbiosis are also discussed here.
3

Selekcja szczepow Rhizobium i Bradyrhizobium odpornych na zakwaszenie podloza i zwiekszona koncentracje glinu

86%
Strzelec A.
Pamiętnik Puławski
|
1992
|
tom 100
201-216
4

The inhibition of Bradyrhizobium sp. [Lupinus] denitrificatory growth by nitrite

86%
Lucinski R., Polcyn W.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
5

Mass spectrometry identification of membrane-bound respiratory nitrate reductase from Bradyrhizobium sp. [Lupinus]

72%
Polcyn W.
Acta Biochimica Polonica
|
2008
|
tom 55
|
nr 4
753-760
Respiratory nitrate reductase (NR) from Bradyrhizobium sp. (Lupinus) USDA 3045 has biochemical properties of the membrane-bound NR type. However, in the completely sequenced rhizobium genomes only genes for the periplasmic type of dissimilatory NR were found. Therefore purification and identification of the enzyme by tandem mass spectrometry (MS/MS) was under taken. MS/MS spectra representing 149 unique tryptic peptides derived from purified 137-kDa subunit matched the NCBInr-deposited NarG sequences. MS/MS sequencing of two other SDS/PAGE bands (65- and 59-kDa) identified them as derivatives of the NarH-type protein. Applying additional validation criteria, 73% of the sequence of the NarG subunit (902 aa) and 52% of NarH sequence (266 aa) was assembled (UniProt KB acc. no. P85097 and P85098). This is the first unambiguous identification of an active NarGH-like NR in rhizobia. Moreover, arguments are provided here for the existence of a functional enzyme of this type also among other rhizobial species, basing on immunoblot screening and the presence of membrane-associated NR-active electrophoretic forms.
6

Rola lipopolisacharydow Rhizobium w tworzeniu efektywnej symbiozy

72%
Glowacka M., Szyprowska S.
Postępy Mikrobiologii
|
1996
|
tom 35
|
nr 4
451-464
7
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Wplyw drobnoustrojow glebowych na rozwoj Rhizobium i Bradyrhizobium i ich symbioze z roslinami motylkowatymi. Czesc II. Wplyw bakterii z rodzaju Pseudomonas

72%
Strzelec A.
Postępy Nauk Rolniczych
|
1995
|
tom 42
|
nr 5
77-87
The data presented in this article were obtained from studies on the effect of pseudomonads on the growth and development of legume plants and on their symbiosis with Rhizobium and Bradyrhizobium. The conducted investigations have proven that pseudomonads are typical microorganisms in the rhizosphere of cultivated plants and show root colonization ability. The effectiveness of the root colonization depends on: the chemical composition of root exudates and their chemotaxic properties; the mobility of the Pseudomonas bacteria cells; the reaction between polysaccharides on the bacterial cell surface and glycoproteins in the surface of the roots; competition abilities of the bacterial strains; soil properties and many other factors. It has been proved that pseudomonads can stimulate, inhibit or show no effect on the development and yield of the host plant. The means by which the bacterial strains can influence plant development depends on the kind and concentration of metabolites produced by the tested strain and the properties of the plant. The mechanism of positive effect of pseudomonads on the plant is the protection of the host plant against its pathogens, due to the abllity of bacteria to produce siderophores (especially of the pseudobactin-pioverdin group) and antibiotics. Some representatives of Pseudomonas can mobilize phosphate ions from poorly soluble inorganic compounds and can release growth stimulators and vitamins. Among the pseudomonads are many phytopathogenic strains as well as many strain negatively influencing plant growth without visible symptoms of disease. This negative influence could be the result of disturbances in the supply to the plant of nutrients and growth stimulators caused by pseudomonads negatively affecting the soll microorganisms from the PGPR group and stimulating microorganisms from the DRMO group.
8

Selekcja szczepow Rhizobium meliloti aktywnych w symbiozie z lucerna nawozona azotem mineralnym

72%
Strzelec A.
Pamiętnik Puławski
|
1992
|
tom 101
89-98
9

Wplyw zapraw nasiennych na rozwoj Rhizobium i Bradyrhizobium oraz na plonowanie szczepionych nimi roslin

72%
Strzelec A., Martyniuk M.
Pamiętnik Puławski
|
1993
|
tom 103
195-208
10
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Wplyw drobnoustrojow glebowych na rozwoj Rhizobium i Bradyrhizobium i ich symbioze z roslinami motylkowatymi. Czesc III. Wplyw grzybow mikoryzowych

72%
Strzelec A.
Postępy Nauk Rolniczych
|
1996
|
tom 43
|
nr 3
19-28
The article describes the classification of vesicular-arbuscular mycorrhizal fungi and their distribution amang legume plants. The results of investigations on the effect of joined inoculation of legume seeds with specific symbiotic bacteria (Rhizobium or Bradyrhizobium) and VAM fungi on growth, nodulation, N₂ fixation and yield of the host plantare discussed. Studies leading to the explanation of mechanisms of the VAM fungi beneficial influence on the symbiosis legume plants with Rhizobium or Bradyrhizobium are also discussed.
11

Localization of expansin-like protein in the apoplast of effective and ineffective pea root nodules

72%
Sujkowska M., Borucki W., Golinowski W.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
12

Effect of active ingredients on Rhizobium and Bradyrhizobium legume dinitrogen fixation

72%
Sawicka A., Selwet M.
Polish Journal of Environmental Studies
|
1998
|
tom 07
|
nr 5
In field experiment the effect of two active ingredients (imazethapyr and linuron) on symbiotic nitrogen fixation activity and microorganisms under legume crops (pea, horse bean, yellow lupine, white lupine, soybean) was determined. The studies indicated that both imazethapyr and linuron can cause decrease of root - nodule bacteria nitrogenase activity. They also can stimulate development of bacteria and inhibit growth of fungi.
13

Yellow lupine gene encoding stearoyl-ACP desaturase - organization, expression and potential application

72%
Zaborowska Z., Starzycki M., Femiak I., Swiderski M., Legocki A. B.
Acta Biochimica Polonica
|
2002
|
tom 49
|
nr 1
A gene for the Δ9 desaturase specific to stearoyl-ACP (acyl carrier protein) was iden­tified from yellow lupine (Lupinus luteus) cDNA and genomic libraries through the dif­ferential display method. The desaturase transcript appears in plants infected with Bradyrhizobium sp. (Lupinus) as revealed by Northern hybridization, RT-PCR and ex­pression of β-glucuronidase under the desaturase promoter. A small amount of desaturase transcript was also detected in uninfected plants, which suggests that the gene does not belong to the strict nodule-specific sequences. The desaturase provides unsaturated fatty acids for additional cell membrane synthesis. During nodule and symbiosome development a peribacteroid membrane is formed and the requirement for membrane surface increases, thus the level of desaturase expression is also higher. Transgenic plants of Nicotiana tabacum with overexpression of the full-length lupine stearoyl-ACP desaturase sequence were obtained. They revealed higher con­tent of unsaturated fatty acids (especially oleic acid) in comparison with control plants.
14

Reakcja szczepów Rhizobium leguminosarum bv. viciae oraz Bradyrhizobium sp.[lupini] na ph w hodowlach na pożywkach sztucznych

71%
Kucharski J., Kuczynska-Krogulec L.
Acta Academiae Agriculturae ac Technicae Olstenensis. Agricultura
|
1996
|
tom 63
59-66
Z brodawek bobiku, peluszki i łubinu uprawianych na glebie o zróżnicowanym pH (od 4.5 do 7.0) wyizolowano bakterie symbiotyczne i w doświadczeniu laboratoryjnym badano wpływ pH 4.0; 5.0; 6.0 i 7.0 na namnażanie się Bradyrhizobium sp. (lupini) i Rhizobium leguminosarum bv. viciae oraz na aktywność dehydrogenaz. Prześledzono także wpływ wapnowania gleby na liczebność bakterii oligotroficznych, makrotroficznych, amonifikatorów, zbiałczających azot i Azotobacter oraz promieniowców i grzybów. W wyniku badań stwierdzono, że dla szczepów Rhizobium leguminosarum bv.viciae (współżyjących z bobikiem i peluszką) optymalnym było pH 7.0, a dla Bradyrhizobium sp. (lupini) - 6.0, przy czym ten ostatni gatunek dobrze znosił zakwaszenie podłoża nawet do pH 4.0. Największą aktywnością dehydrogenaz cechowały się komórki Rhizobium i Bradyrhizobium hodowane na pożywkach o pH 7.0. Wapnowanie stymulowało namnażanie się bakterii oligotroficznych, makrotroficznych, zbiałczających azot i amonifikatorów w glebie spod wszystkich roślin oraz zbiałczających azot i promieniowców w glebie spod peluszki i łubinu. Zabieg ten działał inhibicyjnie na grzyby glebowe oraz na zdolność namnażania się in vitro bakterii Rhizobium współżyjących z bobikiem.
15

Straight and branched (ω-1)-hydroxylated very long chain fatty acids are components of Bradyrhizobium lipid A

58%
Choma A., Komaniecka I.
Acta Biochimica Polonica
|
2011
|
tom 58
|
nr 1
 Lipopolysaccharides of seven Bradyrhizobium strains and three whole-cell fatty acid preparations from bacteria isolated from nodules of Sarothamnus scoparius (Common Broom) were studied for the presence of very long chain (ω-1)-hydroxy fatty acids. Several such fatty acids were identified. Among them, straight-chain as well as mono- and dimethyl branched acids with chains in the range from 26 to 34 carbon atoms were found. Pyrrolidides and 4,4-dimethyloxazoline derivatives were used to determine the branching position. Carbons at the (ω-10) and/or (ω-11) positions in alkyl chains were points of attachment of methyl groups. These data complete the structure of bradyrhizobial lipid A with important details. The obtained results can be applied in the chemotaxonomy of Bradyrhizobium.
16

Use of potato extract broth for culturing root-nodule bacteria

58%
Martyniuk S., Oron J.
Polish Journal of Microbiology
|
2011
|
tom 60
|
nr 4
Liquid media containing potato extract and 1% of glucose or sucrose were used to culture root-nodule bacteria (rhizobia) in shaken Erlenmeyer flasks. For comparison, these bacteria were also cultured in yeast extract-mannitol broth (YEMB) as a standard medium. Proliferation of rhizobia was monitored by measuring optical densities (OD₅₅₀) of the cultures and by plate counting of the viable cells (c.f.u) of the bacteria. In general, multiplication of the rhizobia in potato extract-glucose broth (PEGB) and potato extract-sucrose broth (PESB) was markedly faster, as indicated by higher values of OD₅₅₀, than in YEMB. The numbers of R. leguminosarum bv. vicae GGL and S.meliloti 330 in PEGB and PEGB were high and ranged from 1.2×10¹⁰ to 4.9×10¹⁰ mL⁻¹ after 48 h of incubation at 28°C. B. japonicum B3S culture in PEGB contained 6.4×10⁹ c.f.u. ml⁻¹ after 72 h of incubation. PEGB and YEMB cultures of the rhizobia were similar with respect to their beneficial effects on nodulation of the host-plants of these bacteria.
17

Nitrate-related down-regulation of respiratory nitrate reductase from Bradyrhizobium sp. [Lupinus]

58%
Polcyn W.
Acta Biochimica Polonica
|
2008
|
tom 55
|
nr 4
761-766
Previously, we showed that anaerobic induction of respiratory nitrate reductase (NR) activity in Bradyrhizobium sp. (Lupinus) USDA 3045 is strongly enhanced by nitrate or nitrite through de novosynthesis. Here, multiple NR-active soluble forms, ranging from 75kDa to 190kDa, were observed under anaerobic conditions. Electrophoretic activity band patterns differed depending on the level and the type of the N oxyanion added. The intensity of the membrane-bound NR activity band of 230kDa changed with time along with consumption of 2 mM nitrate. It was associated with a parallel 5-fold increase and then 2-fold reduction in the amount of membrane-bound NR protein. In contrast, on 4 mM nitrate, the level of NR protein was much more stable, apparently due to slower nitrate depletion. Moreover, in cells anaerobically grown without nitrate addition, a 42-kDa derivative of NR degradation was immunodetected, which was not observed if nitrate was present in the medium. These findings suggest that the amount of the respiratory NR protein could be negatively regulated by endogenous proteases in relation to the level of nitrate available. It seems, therefore, that multiple native forms might be not different isoenzymes but immature complexes or derivatives of the enzyme protein turnover. This report adds to a modest list of bacterial enzymes apparently regulated by proteolysis, such as GS, MurAA, EnvA, GdhA, and MetA.
18

Porownanie wplywu szczepienia Bradyrhizobium sp. oraz nawozenia azotowego na plonowanie Arachis hypogae na dwoch roznych glebach

58%
Loc N. B.
Roczniki Gleboznawcze
|
1993
|
tom 44
|
nr 3-4
55-61
19

Insektycydy mikrobiologiczne a rolnictwo ekologiczne

58%
Lonc E., Guz K., Doroszkiewicz W.
Ekonatura
|
2006
|
nr 08
20

Current taxonomy of the Rhizobia

58%
Malek W., Sajnaga E.
Acta Microbiologica Polonica
|
1999
|
tom 48
|
nr 2
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