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W wyciągu metanolowym z ziela L. arvense stwierdzono występowanie 6 związków flawonoidowych: kwercetyny, 3-glukozydu kemferolu (astragaliny), 3-glukozydu kwercetyny (izokwercytryny), glukoramnozydu kwercetyny (rutozydu) oraz glukozydu kwercetyny i glukoramnozydu kemferolu (związków bliżej nieokreślonych). W badaniach wykorzystano chromatografię CC (poliamid i celuluoza), PC i HPLC. Związkiem dominującym w zespole flawonoidów był rutozyd. W zielu L. arvense jego zawartość (określona za pomocą HPLC) wynosiła 0,95%, w liściach 1,18% i w łodygach 0,67%.
Unknown up to the present natural locality of Omphalodes scorpioides from central Wielkopolska (The Jawory Forest near Borek Wielkopolski in the Wielkopolska region) has been presented in this work. It is the fourth record of this species in mentioned region. Also a form of conservation of the population has been defined. Additionally morphological characterization of the species, as well as its occurrence in Poland and Europe have been presented. A special attention was paid to its geographico-historical status in Wielkopolska.
An efficient plant micropropagation system was established using shoot tips and stem segments of Lithospermum canescens and Onosma paniculatum (Boraginaceae). A high frequency of axillary shoot formation was achieved on LS [15] medium with 0.5 mg/1 kinetin and 2.0 mg/1 BAP (average shoot formation per explant was 6.18 ± 1.89 for L. canescens and 3.01 ± 0.82 for 0. paniculatum). The best results were obtained with 1x8 clone of L canescens. Axillary branching and root formation were the highest (8.22 shoot formation, 46.7% of rooting). Contamination of the explants with Rhodococcus fascians D188 increased the number of newly formed shoots of 0. paniculatum to 4.6 ± 0.72, while the propagation process of L. canescens was not affected. The highest percentage of rooted shoots of L canescens was observed on V, B5 medium [14] with 0.2 mg/1 1BA (from 27% to 46.7%, depending on plant line), while in the case of regenerated shoots of 0. paniculatum hormone-free LS medium induced roots more effectively (36.7%) than V2 B5 medium with 0.2 mg/1 1BA (25%).
Analysis of endosperm development in plants from contaminated sites (vicinity of the Żelazny Most copper post-flotation reservoir in the Legnica-Głogów Copper District, and the zinc spoil in Katowice-Wełnowiec) showed general similarities in the pattern of endosperm formation in Echium vulgare, but also deviation from typical haustorium structure (~23% frequency), premature degeneration of the haustorium, or degeneration both of the haustorium and endosperm proper (~40%). The most significant irregularities of endosperm development included lower or higher number of nuclei in two cells of the lateral part of the endosperm, lower ploidy level of haustorium nuclei, and cellularization (instead of coenocytic structure) in the lateral cells of the endosperm. Irregularities and degenerative processes presumably resulted from the stress of environment conditions. Because of the nutritive function of those structures, degeneration or atypical structure of the endosperm and its haustorium in some maturing seeds may reduce the fertility of plants colonizing contaminated sites.
Badano antyfidantne działanie oraz wpływ na rozwoj bielinka kapustnika (P. brassicae L.), alkoholowych i wodnych wyciągów z roślin z rodziny Szorstkolistne (Boraginaceae). Najwyższą aktywność antyfidantną wykazał alkoholowy wyciąg z lepczycy rozesłanej (Asperugo procumbens) i wyciąg wodny z żywokostu lekarskiego (Symphytum officinale). Alkoholowy wyciąg z krzywoszyja polnego (Lycopsis arvensis) i wodny wyciąg z lepczycy, zwiększały ilość pokarmu zużywanego na przyrost 1 mg masy ciała larw. Testowane wyciągi ograniczały rozwój bielinka kapustnika. Stosowane w warunkach polowych, chroniły rośliny przed składaniem jaj przez motyle bielinka kapustnika.
Changes in the composition of fatty acids (FA) were determined in lipid extracts isolated from developing ovaries containing ovules and developing seeds of Echium vulgare L. The samples were collected successively over 20 days beginning with the first day after flowering. The contents of the n-6 FA family members, i.e., γ-linolenic (GLA) (C18:3) and linoleic (LA) (C18:2) acids changed in a parallel manner and reached the maximum of 13.9% and 24%, respectively, on the 12th day, after which they fell systematically down to 8.6% and 18.2%, respectively, on the 20th day after flowering. Starting with day 13, the content of α-linolenic acid (ALA) (C18:3 n-3) begins to grow intensively, from 24.2% to 39.3% on the 20th day after flowering. The increase in the content of stearidonic acid (SDA) (C18:4 n-3), up to 10.5% on the 20th day after flowering, occurred steadily as the seeds developed, and was independent of the changes in the content of GLA and LA. The pattern of changes in the content of SDA, GLA, LA and ALA during the development of seeds, and the occurrence of SDA in the seed oil of other plants, demonstrate that the biosynthesis of SDA in the seeds is critically dependent on the presence of ALA. The above condition indicates that SDA biosynthesis in the seeds of Echium vulgare follows the scheme LA → simultaneous, competitive, action of Δ6 and Δ15 desaturases, leading to the formation of GLA and ALA, respectively, and then ALA (Δ6 des) → SDA. The biosynthesis according to the scheme: GLA (Δ15 des) → SDA is highly unlikely.
The micromorphology of selected elements of Echium vulgare L. fl owers was investigated, with special attention to the structure of the nectaries and the stigma of the pistil as well as types of trichomes occurring on the surface of the calyx. The nectary had the shape of an uneven disc located around the lower region of the four-parted ovary of the pistil. The glandular cells formed a tier with a height of 330 μm and a radial width of 144 μm. Nectar was secreted onto the nectary surface through anomocytic stomata located at the level of other epidermal cells. Most of the stomata were open, with a different dimension of the pore. Their largest number was observed at the base of the nectary, and 462 stomata were noted on the whole surface of the nectary. The cuticle on the surface of the guard cells formed fi ne, circular striae. The subsidiary cells formed striated cuticular ornamentation, with the striae arranged radially in the direction of the stoma, whereas on the surface of other epidermal cells the striae formed an arrangement with different directions. The epidermis on the surface of the stigma formed regularly arranged papillae with a fan-shaped, expanded upper part which had corrugated outer walls, whereas the base of the cell formed a widened small column. The epidermis of the abaxial part of the calyx was covered by numerous non-glandular trichomes of different length which were made up of one or several cells. The glandular trichomes in the epidermis of the calyx grew with smaller density compared to the protective trichomes, and they were composed of a 1-2-celled stalk and a glandular head.
In this study, the micromorphology of nectaries in Echium russicum J. F. Gmel. flowers was determined by using scanning electron microscopy (SEM) and their anatomy by using light microscopy (LM). The rate of nectar production of flowers and sugar concentration in nectar were investigated. The nectary gland is located below the ovary of the pistil. It is composed of 4 parts corresponding to the parts of the ovary. The widest regions of the nectar-producing tissue are situated by the furrows separating the adjacent parts of the ovary. Nectar is secreted through anomocytic stomata, located only in the lower part of the nectary. The stomata were distributed evenly or they formed clusters of 2-3. The average number of stomata on the surface of the whole nectary was 184. At the nectar secretion stage, open and closed, as well as not fully mature stomata were observed. The orientation of most of the stomata was parallel to the nectary base. The cuticle surface of the cells of the upper and lateral part of the nectary was smooth, whereas in the region producing stomata it showed various folds facilitating the retention of nectar. The flowers produced nectar throughout the flowering period. The weight of nectar secreted throughout the lifetime of ten flowers was, on the average, 20 mg, with the concentration of sugars of 58% and their weight reaching 17 mg.
The structure of nectaries in the flowers of Myosotis sylvatica Hoffm. was studied by light microscopy, scanning electron microscopy and transmission electron microscopy. A nectariferous gland of irregular disc shape surrounds the bottom of the four-lobed ovary. From the upper side, both the nectary and the ovary are protected by ligulate folds of a widening corolla tube with epidermis outgrowths. Nectar is secreted by modified stomata situated only on the top and abaxial nectary surface adjoining the corolla tube. Stomata are irregularly distributed, forming aggregates of 2-3 each. On the longitudinal sections of the receptacle, the nectariferous tissue is distinguished by small cells, dense protoplast content, and intercellular spaces of different sizes. Branches of phloem bundles are observed at the base of the nectariferous tissue.
Reproduction of Echium vulgare L. was examined in situ at two contaminated sites (Silesia, Poland): a zinc spoil heap in Katowice-Wełnowiec, and the surroundings of the Żelazny Most copper post-flotation waste reservoir. Plants from uncontaminated sites (near Rymanów) were studied as the control material. We compared the reproductive capacity of plants in the stress conditions to that of plants from uncontaminated sites. Degenerative processes and abnormalities found in the reproductive organs of plants from polluted sites, more intensified in the population from Żelazny Most, resulted in lowered fertility of plants. In germination tests on standard soil and from polluted sites, seeds from the control plants had the lowest germination on soil from Żelazny Most, and seeds from Żelazny Most had significantly delayed germination and had higher germination on standard soil than on polluted soil from their site; on the other hand, seeds from Katowice-Wełnowiec had higher germination on waste heap soil than on standard soil. The results suggest that among the populations from polluted sites, the one from Katowice-Wełnowiec is more advanced in selection for tolerance.
Nectar secretion and nectar chemistry in the flowers of comfrey (Symphytum officinale L.) were examined in the four stages of anthesis: I-large buds, before pollen exposure; II- freshly opened flowers with the beginning of anther dehiscence; III- completely opened flowers in the maximum of pollen exposure; IV- flowers at the final stage of anthesis, without pollen in the anthers. Individual flower stays fresh 2.5-3 days, on average. Disc-shaped nectaries of S. officinale are located at the base of the four-lobbed ovary. Nectar is released through the modified stomata. Start of nectar secretion was noted at the bud stage. Nectar volume, mass of nectar and sugars differed in the examined stages of anthesis and the biggest values were noted at the final stages. Dominant sugar in nectar was sucrose with smaller amounts of fructose and glucose. The presence of amino acids was also recorded.
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