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The effect of biopreparation Biochikol 020 PC on linear growth of mycelia of two Rhizoctonia isolates was tested under laboratory conditions along with that of fungicide Baytan Universal 19,5 WS for comparative purposes. Complete retardation of mycelial growth of both isolates was observed in case the application of fungicide Baytan Universal 19,5 WS at the concentration of 0.1% and a considerable growth inhibition was stated at the temperatures of 10°C and 30°C. The effect of seed dressing with the above mentioned preparations on plant infestation by Rhizoctonia fungi was tested in a greenhouse experiment on two cultivars of spring wheat: Banti and Ismena. Baytan Universal 19,5 WS reduced plant infestation especially at the highest soil moisture content of 20 kPa. Biopreparation Biochikol 020 PC and diversification of soil moisture levels did not result in a reduction of wheat infestation rate. This biopreparation stimulated the increase of root mass of both cultivars at moisture content of 20 kPa.
The object of a field experiment conducted in the years 2003–2004 was the rhizosphere soil of soybean ‘Mazowia’ cv. Before the sowing, the seeds were dressed with biopreparations (Biochikol 020 PC, Biosept 33 SL, Polyversum) or fungicide (Oxafun T). The non-dressed seeds constituted the control. The microbiological analysis of the rhizosphere soil of soybean, carried out at anthesis, showed that the greatest number of bacteria in total and bacteria Bacillus spp. and Pseudomonas spp. occurred after the application of Biochikol 020 PC, while the lowest occurred in the control combination. The smallest number of fungi was observed after the application of Biochikol 020 PC or Oxafun T, slightly bigger after the introduction of Biosept 33 SL or Polyversum into the soil, while the biggest in the control combination. Fungi from the genera of Alternaria, Fusarium, Pythium and Gliocladium, Penicillium, and Trichoderma were most frequently isolated from the rhizosphere of soybean. After the application of biopreparations the proportion of pathogenic fungi in the rhizosphere of soybean was much smaller than in the control. The highest proportion of Gliocladium spp. occurred after the application of Polyversum or Biochikol 020 PC, while that of Trichoderma spp. after the introduction of Biochikol 020 PC or Biosept 33 SL. Besides, tests on dishes showed that the biopreparations and the fungicide used in the experiment contributed to increased numbers of antagonistic bacteria (Bacillus spp. and Pseudomonas spp.) and fungi (Gliocladium spp. and Trichoderma spp.).
There was tested an influence of three biostymulants Atonik SL, Biochikol 020 PC and Tytanit on yield and fruits quality of three fruiting repeating Polish raspberry cultivars: Pokusa, Polka and Poranna Rosa during years of 2005–2006. These agents had a positive influence on fruits quality and yielding to all tested cultivars. The fruits with exception of Tytanit treated fruit especially in the beginning of harvest, were characterized by better firmness and were bigger. However, Tytanit treatment increased soluble solids content in fruits and reduced in Polka cv. nitrates concentration. Among tested cultivars, cv. Polka was characterized by the highest yield, that was of c. a. 50% greater that of cv. Pokusa, which was characterized by the gratest fruits sizes. The ‘Poranna Rosa’ shawed the least soluble solids and vitamin C content and had the highest acidity.
A field experiment was conducted in the years 2003-2004 on a field of 10-years’ soybean monoculture, with naturally accumulated infection material in the soil. Soybean of ‘Mazovia’ was the object of studies. Before the sowing the seeds were dressed, and at the beginning of anthesis the plants were sprayed with biopreparations Polyversum, Biosept 33 SL and Biochikol 020 PC. The experiment considered the combination with dressing the seeds by means of Zaprawa Oxafun T (a.s. carboxine 37.5% + tiuram 37.5%) and spraying the plants with fungicide Bravo Plus 500 S.C. (a.s.: chlorotalonile 50%). The seeds that were not dressed constituted the control. During the vegetation the number and healthiness of soybean plants were determined twice (in the seedling phase and at anthesis), at the same time a laboratory mycological analysis was carried out. After the harvest the studies established the size and quality of the seed yield of soybean growing in particular experimental combinations. The best number of plants was obtained in the combination with Biosept 33 SL, slightly weaker after applying Polyversum or Zaprawa Oxafun T, and the worst in the control combination. Biosept 33 SL and the fungicide were the most effective in protecting the plants from infection by soil-borne fungi. Polyversum and Biochikol 020 PC also showed a good protective effect. The greatest seed yield was collected from soybean plants after applying Biosept 33 SL, while the lowest – from control plants. Soybean plants were mainly infected by Fusarium spp.,A. alternate, P. exigua, R. solani and P. irregulare. Those fungi were most frequently isolated from control plants, and the least frequently after applying Biosept 33 SL or Polyversum. The proportion of pathogenic fungi (P. sojae, Fusarium spp., S. sclerotiorum, P. exigua) in seed infection of soybean was the smallest in the combinations with Biosept 33 SL or Zaprawa Oxafun T, while the biggest in the control.
This study presents the effect of biopreparations (Polyversum, Biochikol 020 PC and Biosept 33 SL) on fungal and bacterial rhizosphere and non-rhizosphere communities after seed dressing and spraying of Phaseolus vulgaris plants. The use of biopreparations has a positive effect on the communities of bacteria and fungi in soil under the cultivation of this plant. The number of cfu of the studied microorganisms in the nonrhizosphere soil was slightly lower than in the rhizosphere. Biochikol 020 PC and Biosept 33 SL increased the number of cfu of bacteria Bacillus spp. and Pseudomonas spp. and decreased the population of soil-borne fungi. Different species were isolated within the fungi and they belonged to the following genera: Altenaria, Fusarium, Rhizoctonia, Sclerotinia, Gliocladium, Penicillium and Trichoderma. The most antagonistic bacteria and fungi were obtained after introducing biopreparations Biochikol 020 PC or Biosept 33 SL. The smallest number of antagonists were found in the soil after dressing the bean seeds with Zaprawa Oxafun T and spraying the plants with fungicide Bravo Plus 500 SC and in the control combination. Besides, the applied biopreparations and fungicides had a positive effect on Pheseolus vulgaris yielding.
In presented studies was undertaken the attempt to determine the effectiveness of such preparations as Biochikol 020 PC and Biosept 33 SL in limiting the growth of T myrtilli in vitro. Moreover, the protective effect of these biopreparations and Dithane M45 80WP for highbush blueberry stems was determined. The studies carried out in vitro indicated that Biochikol 020 as well as Biosept 33 SL significantly limited the growth and development of pathogen. Very strong inhibition of hyphae growth and its deep degradation were caused by Biosept 33 SL in concentration 0.3%. Similarly, in experiment on protecting action of selected preparations for stems of highbush blueberry against T. myrtilli, Biochikol 020 and Biosept 33 SL inhibited the growth of pathogen. But its protective effects were weaker in comparison to Dithane M45 80WP because after the application of this preparation the development of disease symptoms caused by T. myrtilli was significantly lower.
The subject of the studies concerred pathogenic fungi (Alternaria alternata 17, Ascochyta pisi 31, Botrytis cinerea 19, Fusarium culmorum 44, F. oxysporum f. sp. glycines 37, F. oxysporum f. sp. phaseoli 51, F. oxysporum f. sp. pisi 33, Fusarium solani 25, Phoma exigua var. exigua 11, Pythium irregulare 34, Rhizoctonia solani 64, Sclerotinia sclerotiorum 15) and bio-preparations, i. e. Biosept 33 SL, Biochikol 020 PC, Polyversum as well as Zaprawa Oxafun T. The studies were conducted in in vitro conditions, considering 6 repetitions (dishes) for each experimental combinations. After 10 days the diameter of the fungus colonies’ growth was measured and morphological changes in the appearance of fungi cultures were observed. Addition of a medium of bio-preparations and Zaprawa Oxafun T caused inhibition of the growth and development of pathogenic fungi. The most effective preparation which inhibited the growth of the colonies was Biosept 33 SL. Biopreparations as well as Zaprawa Oxafun T caused inhibition of the formation of air mycelium, sporules and sclerotia.
The influence of biopreparations Bioczos SL, Biosept 33 SL, Biochikol 020 PC, Propolis and synthetic fungicides Euparen Multi 50 WG, Horizon 250 EW and Teldor 500 SC was examined on the growth of micorrhizal fungus Oidiodendron sp. Fungicides were added to PDA medium at the following concentrations: recommended dose, 5-times lower than the recommended and 5-times higher than the recommended. The synthetic fungicides were more toxic to Oidiodendron sp. than biopreparations. On the medium containing Horizon 250 EW fungus did not growth. The greather part of biopreparations inhibited growth of Oidiodendron sp. but only Bioczos SL and Propolis in dose 5-times higher than the recommended, toataly inhibited growth of fungus colonies.
The purpose of the studies was to determine the effect of such preparations as Biochikol 020 PC, Polyversum and Biosept 33 SL on the formation of microorganisms communities in the rhizosphere soil of soybean. Results of the studies pointed to a positive effect of biopreparations on the increase number of cfu total bacteria, Bacillus spp. and Pseudomonas spp. After the application of biopreparations balance between pathogens and saprobionts was maintained in the rhizosphere soil of soybean. Such relation did not exist after using chemical preparations. Biopreparations increased the number of antagonistic bacteria – Bacillus spp., Pseudomonas spp. and fungi – Gliocladium spp., Trichoderma spp.
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