Wyniki wyszukiwania - AGRO

1

Zastosowanie modelu shift log do oceny tempa rozpadu białka Cry1Ab w glebie

100%

Zmijewska E., Linkiewicz A. M., Sowa S., Nowosielski J., Zurawska-Zajfert M., Gralewska-Nowotko K., Zimny J.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2019

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nr 285

2

Enhancement of pathogenicity of Bacillus thuringiensis by gamma rays

88%

Khan K. I., Jafri R. H., Ahmed M.

Polish Journal of Microbiology

|

2004

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tom 53

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nr 3

Studies were conducted on the enhancement of pathogenicity of Bacillus thuringiensis by gamma rays on various species of termites. The results showed that the 72 hrs old and 60 or 70 kr irradiated culture of B. thuringiensis were more pathogenic than the non-irradiated ones. 168 hrs old and 150 kr (1.5 kGy) irradiated B. thuringiensis caused quicker mortality rate than the non-irradiated one as it shows maximum enhancement ratio (1.32).

3

Selective isolation of Bacillus thuringiensis from soil by use of L-serine as minimal medium supplement

88%

Andrzejczak S., Lonc E.

Polish Journal of Microbiology

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2008

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tom 57

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nr 4

The influence of L-serine on the growth of different strains of the genus Bacillus was investigated. It has been observed that the addition of L-serine to minimal synthetic media results in an inhibition in the growth of certain strains of Bacillus spp. but not B. thuringiensis. Then L-serine-resistance phenomenon was used in isolation of B. thuringiensis strains from soil. An isolation method with media supplied with L-serine was compared to the previously applied procedure (isolation on nutrient agar). L-serine-selective medium appeared to be more effective in isolation of Bt strains.

4

Biodiversity of arthropod pathogens in the Bialowieza Forest

88%

Sosnowska D., Balazy S., Prishchepa L., Mikulskaya N.

Journal of Plant Protection Research

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2004

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tom 44

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nr 4

In the years 2002–2004 strains of Bacillus thuringiensis and 37 species of entomopathogenic fungi were isolated and identified in the Polish and Belarussian parts of Białowieża Forest (BF). Mitosporic fungi and bacteria dominated in litter superficial soil layer, forest, litter and floor vegetation whereas entomophtoralen fungi prevailed in bushy undergrowth layers and tree crowns. The dominant species Beauveria bassiana was observed in forest floor, subcortical habitats on dead trees, meadows and rushes. The species Entomophthora israelensis, Beauveria cf. bassiana, Paecilomyces suffultus and P. tenuipes were for the first time described as insect pathogens in BF. Entomophthorales seem to hold much greater part than mitosporic forms in the whole diversity of entomopathogenic fungi. Relatively rich sets of these fungi recognised in BF during last decades confirm the predestination of this area as highly significant refuge for other groups of arthropod pathogens, and it should encourage scientists to widen their research and contribute to a rather scarce knowledge in this field.

5

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Bezpieczeństwo ekologiczne komarobójczych biocydów Bacillus thuringiensis israelensis

75%

Rydzanicz K., Lonc E.

Wiadomości Parazytologiczne

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2010

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tom 56

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nr 4

6

Optimization of process parameters for maximum poly[-beta-]hydroxybutyrate [PHB] production by Bacillus thuringiensis IAM 12077

75%

Adwitiya P., Ashwini P., Avinash A. K., Badri R., Dadhe K., Ponnamma V., Shivakumar S.

Polish Journal of Microbiology

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2009

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tom 58

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nr 2

149-154

The study aimed at screening and identifying a potential poly-β-hydroxybutyrate (PHB) accumulating Bacillus strain and optimization of media parameters for increased PHB production by the strain. A Gram-positive bacterium that accumulated PHB was isolated from local garden soil of Bangalore. Based on morphological and physiological properties, and nucleotide sequence (about 1.5 kb) of its 16S rDNA it was identified as Bacillus thuringiensis IAM 12077. PHB production was found to be comparable to most of the Bacillus sp. reported to date. PHB production by this strain was dependent on nutrient limitation. Cell dry weight and PHB accumulation increased significantly under biphasic growth condition (from nutrient broth to nitrogen-deficient medium) as compared with growth in nutrient broth alone (from 0.32 g/l to 2.76 g/l cell dry weight; 24% to 43.37% PHB accumulation; 0.2 g/l to 1.2 g/l PHB production), with maximum accumulation at 24 h in nitrogen-deficient medium. Time course study of growth and PHB production by this strain in the nitrogen deficient medium showed that PHB production was associated with the stationary phase of growth. All the tested media containing different carbon and nitrogen sources supported growth and PHB production. Ultraviolet spectrum of the extracted polymer showed a characteristic peak at 235 nm.

7

Owadobójcze bakterie

75%

Pniak M.

Hasło Ogrodnicze

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2011

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nr 02

8

Owadobojcze toksyny Bacillus thuringiensis

75%

Misztal L. H., Musial W. G., Augustyniak J.

Postępy Mikrobiologii

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1996

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tom 35

|

nr 2

193-211

9

Crystalline protein profiling and cry gene detection in Bacillus thuringiensis strains isolated during epizootics in Cydia pomonella L.

75%

Konecka E., Baranek J., Kaznowski A.

Biological Letters

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2014

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tom 51

|

nr 2

10

Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis

75%

Briza J., Pavingerova D., Vlasak J., Niedermeierova H.

Acta Biochimica Polonica

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2013

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tom 60

|

nr 3

Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.

11

Wrażliwość aksenicznych larw Galleria mellonella L. na biopreparaty zawierające Bacillus thuringiensis Berliner

75%

Szczepanik M.

Przegląd Zoologiczny

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1989

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tom 33

|

nr 3

12

Nieprzyjazne pasozytom a przyjazne srodowisku trangeniczne bioinsektycydy

75%

Kucinska J., Lonc E., Rydzanicz K.

Wiadomości Parazytologiczne

|

2003

|

tom 49

|

nr 1

11-20

Transgenic bioinsecticides inimical to parasites, but imical to environment. Identification of Bacillus thuringiensis (Bt) parasporal crystalline inclusions composed of Cry proteins (=delta-endotoxins) resulted in introduction of microbial pesticides for biological control of some parasites. Delta-endotoxins are encoded by cry genes and are active against pest and nuisance insects (mostly mosquitoes and black flies - vectors of still important infectious diseases). The recent significant progress in DNA recombination technique may overcome limitations (a short residual persistence and a narrow spectrum of activity) associated with application of Bt conventional products. An introduction of cry genes from mosquitocidal subspecies B. th. israelensis (Bti) to the aquatic microorganisms inhabiting the same water bodies as mosquito and fly larvae (Diptera), has considerably improved the toxin delivery system to target insects. However, in the first experiments, in which Bti genes were cloned in cyanobacteria (Agmenellum quadruplicatum, Synechocystis PCC6803), a low gene expression was observed. Thus, it was necessary to integrate cry genes with strong promoters or to increase the number of vector-introduced copies. To overcome the obstacles of low gene expression and regulatory restriction for 1ecombinant organisms, Bti spore/crystal formulations were encapsulated in the aquatic protozoan, Tetrahymena pyriformis. Large numbers of crystals (180 to 240/cell) were accumulated in its food vacuoles. This system resulted also in an increase in toxin persistence from 24 to 71 h. Cloning Bti genes in B. sphaericus (which also produces mosquitocidal proteins) was another way of an increasing Bt crystal residual activity. In this case, the crystals were additionally protected by B. sphaericus exosporium. These transgenic bacteria produced large amounts of delta-endotoxins that remained under water surface longer than the wild B. sphaericus strains. Moreover, they had a broader spectrum of insecticidal activity, because B. Sphaericus is toxic mostly to Culex and Anopheles, and Bti - mostly to Culex, Aedes and some Simmulidae. Gram-negative bacteria (Asticcacaulis excentricus, Caulobacter crescentus and Ancylobacter aquaticus) turned out also to be effective delta-endotoxin producers. They grow on simple media and do not contain proteases which could degrade Cry proteins. In some cases, 100% mosquito larvae mortality was observed as a result of an exposure to transgenic microorganisms containing Bti genes. However, transgenic techniques are still not very popular in the world, despite their efficacy in biological control of insects. The transgenic organism construction is expensive and time-consuming. Genetic engineeling is still raising a lot of anxieties and doubts concerning inappropriate use of modified organisms. On the other hand, this technology could solve many problems associated with vectors of important diseases, which are still unapproachable to contemporary medicine.

13

Preparaty bakteryjne do zwalczania szkodnikow na dzialce

75%

Ignatowicz S.

Działkowiec

|

1998

|

nr 07

37

14

Mozliwosci zwalczania szkodliwych muchowek w pieczarkarni za pomoca biopreparatow zawierajacych zarodniki i krysztaly bialkowe laseczki turyngskiej

75%

Ignatowicz S., Lewandowski M.

Biuletyn Producenta Pieczarek - Pieczarki

|

1998

|

tom 26

|

nr 2

32-35

15

Isolation and characterization of bacterial endophytes of Chelidonium majus L.

75%

Goryluk A., Rekosz-Burlaga H., Blaszczyk M.

Polish Journal of Microbiology

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2009

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tom 58

|

nr 4

355-361

The aim of this study was to isolate and identify endophytic bacteria from sterns of Chelidonium mqjus L. (greater celandine) and to evaluate their antifungal properties. In total, 34 bacterial endophyte strains were isolated. The fungistatic effects of these bacteria on the growth of five moulds (Alternaría altérnala, Chaetonium sp., Paecilomyces variotti, Byssochlamys fitlva, Aureobasidium pullulans) and one species of black yeast (Exophiala mesophila) were tested. The majority of the bacterial isolates were found to inhibit the growth of fungi and those with the strongest antifungal properties were further characterized. Of the twelve isolates examined, 11 were species of Bacillus thuringiensis and one was Bacillus amyloliquefaciens.

16

Wykorzystanie bakterii Bacillus thuringiensis do produkcji bioinsektycydów

75%

Konecka E., Kaznowski A., Baranek J.

Postępy Mikrobiologii

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2011

|

tom 50

|

nr 4

17

Pierwsza Międzynarodowa Konferencja nt. Bacillus thuringiensis "Bt 1991"

75%

Lonc E.

Wiadomości Parazytologiczne

|

1991

|

tom 37

|

nr 4

18

Biodiversity of Bacillus thuringiensis strains in the phylloplane and soil of Lower Silesia Region [Poland]

75%

Doroszkiewicz W., Lonc E.

Acta Microbiologica Polonica

|

1999

|

tom 48

|

nr 4

19

Differentiated pattern of protein composition of crystalline inclusions of newly isolated Bacillus thuringiensis strains from Silesia in Poland

75%

Guz K., Kucinska J., Lonc E., Doroszkiewicz W.

Polish Journal of Microbiology

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2005

|

tom 54

|

nr 4

Protein profiles of crystal delta-endotoxins were determined in twenty nine Bacillus thuringiensis strains-soil and phylloplane isolates - from Poland. Electrophoretic analysis revealed quantatively and qualitatively different patterns of delta-endotoxin crystal preparations of these B. thuringiensis strains. The crystalline parasporal inclusions of B. thuringiensis isolates were composed of two, three, four or five proteins. Molecular weights of these polypeptides varied from 23.4 kDa to 142 kDa. There is lack of correlation between serovars of B. thuringiensis strains, the morphology of crystals and the number and size of proteins in parasporal inclusions.

20

Genetic diversity among Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis strains using repetitive element polymorphism-PCR

75%

Brumlik M. J., Bielawska-Drozd A., Zakowska D., Liang X., Spalletta R. A., Patra G., Delvecchio V. G.

Polish Journal of Microbiology

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2004

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tom 53

|

nr 4

Repetitive element polymorphism-PCR (REP-PCR) is one of the tools that has been used to elucidate genetic diversity of related microorganisms. Using the MB1 primer, REP-PCR fingerprints from 110 Bacillus strains within the "B.cereus group" have identified eighteen distinct categories, while other more distantly related bacterial species fell within six additional categories. All Bacillus anthracis strains tested were found to be monomorphic by fluorophore-enhanced REP-PCR (FERP) fingerprinting using the MB1 primer. In contrast, other non- B.anthracis isolates displayed a high degree of polymorphism. Dendrogramic analysis revealed that the non- B.anthracis strains possessing the Ba813 chromosomal marker were divided into two clusters. One of the clusters shared identity with the B.cereus strains examined.

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