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We assessed the genetic diversity and phylogeography of the Azores Blackbird Turdus merula, based on sequences of two mitochondrial genes (Cytochrome b and NADH Dehydrogenase subunit 2) and one nuclear gene (Aconitase 1 - intron 9) from 45 individuals and an outgroup of 15 birds from Madeira, continental west Europe and north Africa. Our results revealed the lack of genetic structure on these islands and the presence of, at least, two different lineage groups that may indicate two different founder events of the Azores by Blackbirds.
Although research on environmental gradients is relatively common, the altitudinal gradient is one of the least explored. The discrepancies between earlier studies attributing highest diversity to lowest altitude and more recent studies where this altitudinal pattern is not maintained justify the pertinence of investigating the way diversity gradually changes with altitude. The studied altitudinal gradient is located inside the volcanic cone, on one of volcanic island Azores, and the investigated altitudes were 450, 600 and 800 meters a.s.l. Malaise traps were used to capture insects and Diptera were selected for this study. Application of the Zipt-Mandelbrot model to rank-abundance curves of Diptera sampled at three different altitudes, as well as analyses of richness, diversity and evenness, led to the conclusion that medium altitude was the stratum with the highest niche diversity, the highest specific diversity and the highest predictability. Non-metric multidimensional scaling allowed an excellent separation of the three altitudes sampled, suggesting that habitat characteristics significantly differ with altitude. The study, undertaken in a volcanic island of the Azores, Atlantic Ocean, will have heuristic interest to the scientific community in general and especially to those researchers studying environmental gradients. The results of this research will underpin further work on the insect biodiversity in the Azores Archipelago.
To determine allele frequencies, surveys of coat phenotypes of domestic cats were conducted in Madeira Island (Madeira archipelago) and Flores Island (Azores archi­pelago). The samples fitted a 1:1 sex ratio and panmitic populations were assumed based on the analysis of the O locus. The genetic profiles were compared to those from other Atlantic littoral populations and a marked deviation was detected at Flores Island referring to very high values of d, XV and I alleles. The differences were interpreted as indicating that cats from Flores may reflect the condition of continental cats in former times. The Madeira population is more similar to Western Europe probably due to more close and continual contact in recent years.
Water frogs, Pelophylax perezi, that are introduced in the Azores, were screened for parasites using PCR primers known to amplify Apicomplexa parasites, and using nematode-specific primers. With the former, three different organisms were detected: Hepatozoon, a trichodinid protozoan ciliate and a possible Stramenopile. Using the latter set of primers, a single unknown spirurid nematode was also detected. Phylogenetic analyses indicate that Hepatozoon detected within amphibian hosts appear to form a clade, although relationships of these parasites do not match the vertebrate intermediate host phylogeny. Regarding the possible Stramenopile, it is unclear whether this organism was actually present on the amphibian or in the water on the surface of the tissue sample. Our findings highlight that many different organisms can be detected with these primers and that they can be used to screen introduced host populations to detect parasites that have been brought with them.
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