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The study focussed on differentiation of hepatic and pancreatic cells in asp (Aspius aspius L.) larvae during early ontogeny (1–21 days post hatch). Histological evaluations and histochemical assays showed the asp liver and pancreas to commence functioning almost simulatenously, between the third and the fifth day of larval life.
The aim of the study was to determine the basic quality parameters and selected biochemical markers of asp, Aspius aspius (L.), semen after spermiation was stimulated with Ovaprim and Ovopel. Sperm motility and concentration, osmotic pressure, total protein content, and activities of acid phosphatase (AcP), lactate dehydrogenase (LDH), and b-N-acetylglucosaminidase (b-NAG) were determined. It was revealed that higher sperm motility and concentration and higher seminal plasma protein content were obtained after stimulation with Ovaprim. The osmotic pressure of the seminal plasma estimated for males following the administration of Ovopel was higher than after they had been treated with Ovaprim. It was determined that enzyme activity in the seminal plasma of the fish stimulated with Ovaprim was higher in comparison with results obtained after they had been treated with Ovopel. Significant, positive dependencies were confirmed between the concentration of sperm and the total protein content in the seminal plasma (r2 = 0.492) and the activity of b-NAG (r2 = 0.779); among the total protein content in the seminal plasma and the activities of AcP (r2 = 0.476), LDH (r2 = 0.564), and b-NAG (r2 = 0.738); and between the activities of AcP and LDH (r2 = 0.483) and between the activities of LDH and b-NAG (r2 = 0.844).
Age and growth rate of asp (Aspius aspius) from the Pierzchały Reservoir were estimated by back calculations based on scale readings (annual rings identification). The scale reading procedure was complicated by presence of additional rings, called "juvenile" (these appearing within the first year′s increment) and "spawning" (present on scales taken from individuals older than 3 years). These additional structures were not characteristic for all the examined individuals. Localisation of the first annual ring was complicated by fairly fast growth rate during winter, so the typical zones of narrow sclerites, indicating slow-growth season did not occur, affecting the scale readability. The quantitative share of asp in control catches amounted to 9%. Most of these individuals (70%) were to 4 to 7 years old. The oldest asps were aged 12, at body length of 66 cm and body weight of 4500 g. The Pierzchały Reservoir is merely exploited by fishermen.
Artificial reproduction of asp under controlled conditions was done using two different spawning agents based on GnRH analogues and dopamine antagonists (Ovopel and Ovaprim). Fish in the Ovopel and Ovaprim and combined treatment groups were treated with a dose equivalent to 1.2 pellets (0.2 and 1.0), 0.5 cm³ liquid (0.1 and 0.4) and 0.2 pellets and 0.4 cm³ liquid per kg of body weight respectively. The highest percentage of ovulation (100%) and embryo-survival to the eyed-egg-stage (81.3%) was recorded after the application of a combination of Ovopel and Ovaprim in comparison with other groups. Fish from the control group did not ovulate. The latency time was shorter in the groups where Ovopel and Ovopel with Ovaprim was applied (40) than in Ovaprim group (42–44 hrs). The obtained results indicates that combination of Ovopel with Ovaprim might be successfully used for artificial reproduction of asp.
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