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Investigation of the presence of Aspergillus flavus in mastitis milk with PCR

100%

Sukru K., Ugur P., Ugur A., Tugba Y. H.

Environment, Earth and Ecology

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2019

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tom 3

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nr 1

2

Warunki rozwoju Aspergillus flavus i wytwarzania aflatoksyny B1 w majonezach

100%

Burzynska H.

Roczniki Państwowego Zakładu Higieny

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1973

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tom 24

|

nr 1

3

The susceptibility of Aspergillus and Penicillium to recent antimycotics

100%

Macura A. B., Pawlik B., Szczepka I.

Wiadomości Parazytologiczne

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1998

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tom 44

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nr 3

4

Entomopathogenic fungi in populations of the satin moth Stilpnotia salicis L.

100%

Ziemnicka J., Sosnowska D.

Journal of Plant Protection Research

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1997

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tom 37

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nr 1-2

Six fungal species, Aspergillus flavus Link ex Fr., Beauveria bassiana (Bals.) Vuill., Acremonium sensu Gams, Fusarium sp. Link ex Fr., Paecilomyces farinosus (Holm. ex Gray) Brown et Smith as well a Paecilomyces fumosoroseus (Wize) Brown et Smith, have been isolated as result of studies on entomopathogenic fungi of the satin moth Stilpnotia salicis. The epizootic induced by the fungus B. bassiana in 1994 caused about 92% reduction of the pest population. That has been noted for the first time in a natural population of S. salicis in Poland.

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Właściwości szczepów Aspergillus flavus LINK izolowanych z gleb i roślin uprawnych południowej Polski. Część I. Częstotliwość występowania i właściwości biochemiczne

94%

Chmiel M. J.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2011

|

tom 567

W pracy oszacowano częstotliwość występowania Aspergillus flavus na terenach rolniczych oraz oceniono właściwości biochemiczne wyizolowanych szczepów. Grzyby izolowano z gleby i roślin uprawnych w okresie wegetacyjnym z terenów południowej Polski. Łącznie przebadano 500 próbek. Oceniano zdolność izolatów do wykorzystywania różnych źródeł węgla, wzrost w różnych warunkach temperaturowych, ciśnieniu osmotycznym i odczynie pożywki. Obecność A. flavus stwierdzono jedynie w 11 próbkach roślin i 3 próbkach badanej gleby (odpowiednio w 4,4% oraz 1,2% próbek). Wszystkie szczepy charakteryzowały się dobrym wzrostem na pożywkach zawierających różne źródła węgla i zbliżoną reakcją na ciśnienia osmotyczne, temperaturę hodowli i odczyn pożywki. Optymalna temperatura wzrostu mieściła się między 20 a 35°C, a odczyn przy którym oznaczony był maksymalny przyrost biomasy w zakresie 5 do 8. Przy ciśnieniu osmotycznym powyżej 30 tysięcy hPa rozwój Aspergillus flavus został zahamowany. Tylko jeden szczep istotnie różnił masą produkowanej grzybni bez względu na warunki hodowli.

6

Fungal colonisation of tracheotomy tubes in patients diagnosed with larynx cancer

84%

Gniadek A., Krzysciak P., Hawryszuk A., Wojcikowska K.

Annals of Parasitology

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2016

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tom 62

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nr Suppl.

7

Isolation and identification of a new fungal strain for amylase biosynthesis

84%

Bakri Y., Magali M., Thonart P.

Polish Journal of Microbiology

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2009

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tom 58

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nr 3

269-273

Fungi are well known for their ability to excrete enzymes into the environment. The fungal isolate FSS60 was the best amylase producer among one hundred and thirty-six isolates obtained from Syrian soils and tested for amylase production. According to the sequence of the internal transcribed spacer (ITS) rDNA gene, the isolate was identified as Aspergillus flavus. Optimal initial pH for amylase production was found to be 9.0. The enzyme was optimally active at 50°C and pH 5.0.

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Mapowanie mikologicznych zagrozen srodowiska z zastosowaniem GIS-u

84%

Lonc E., Wieliczko A., Plewa K.

Wiadomości Parazytologiczne

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2009

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tom 55

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nr 4

425-428

9

Analysis of molecular variability among isolates of Aspergillus flavus by PCR-RFLP of the ITS regions of rDNA

84%

Mohankumar M., Vijayasamundeeswari A., Karthikeyan M., Mathiyazhagan S., Paranidharan V., Velazhahan R.

Journal of Plant Protection Research

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2010

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tom 50

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nr 4

A total of seventeen isolates of Aspergillus flavus from maize were collected from different agro-ecological zones of Tamil Nadu, India. The isolates were tested for their ability to produce aflatoxin B1 (AFB1) in vitro by indirect competitive enzyme-linked immunosorbent assay (ELISA). The amount of AFB1 produced by the isolates of A. flavus ranged from 1.9 to 206.6 ng/ml. Among the various isolates of A. flavus, the isolate AFM46 produced the highest amount of AFB1. DNA was extracted from A. flavus isolates and their molecular variability was investigated by using restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified internal transcribed spacer (ITS) regions of ribosomal DNA. PCR amplification with ITS1 and ITS4 primers resulted in the amplification of a product of approximately 600 bp. Digestion of the PCR products with the restriction enzymes EcoRI, HaeIII and TaqI produced fragments of different sizes. Analysis of the genetic coefficient matrix derived from the scores of RFLP profiles showed that minimum and maximum per cent similarities among the tested A. flavus strains ranged from 0 to 88%. Cluster analysis using the unweighted pair-group method with arithmetic average (UPGMA) clearly separated the isolates into five groups (group I–V) confirming the genetic diversity among the A. flavus isolates from maize.

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Czy plesnie w zywnosci sa szkodliwe dla zdrowia?

84%

Kafel S.

Zdrowa Żywność - Zdrowy Styl Życia

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1999

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nr 4

9-10

11

Influence of N,N-bis[3-aminopropyl]dodecylamine on the mycelium growth and the cell wall composition of resistance and sensitive strains belonging to the genus Aspergillus

84%

Kozirog A., Kuberski S., Zakowska Z., Brycki B.

Polish Journal of Microbiology

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2005

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tom 54

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nr 4

Resistance causes of moulds to N,N-bis(3-aminopropyl)dodecylamine (APDA) for selected species of Aspergillus niger and Aspergillus flavus was examined. Control (sensitive) strains and resistant strains, cultured at 0.05% triamine, were used in the experiments. The non-resistant strains did not have growth capacity in this amount of ADPA while the resistant strains were characterised by a smaller biomass increase. Individual stages of the development of the mycelium occurred later than those in the control samples. The participation of the cell wall in the mycelium biomass of the resistant strains was higher by 7.5%. The glucan content in the wall dry mass was lower by 11% than that in the sensitive strains. A 41% increase in the lipid content was recorded in the cell wall of resistant Aspergillus flavus. A 21 % protein increase occurred in the wall of Aspergillus niger comparing to the control strain. Infrared spectrophotometry analysis of the cell wall did not reveal the presence of triamine. Most absorption bands disappeared in the wall of Aspergillus flavus while no additional absorption bands were registered in Aspergillus niger, some bands were only stronger than those in the control sample. The resistant strains were characterised by a smaller ergosterol content, the main constituent of cell membranes. Spectrophotometry analysis of the mycelium did not reveal significant qualitative changes; only quantitative changes were observed. It was noticed that the resistance reaction did not occur with the same intensity in both species studied. The resistant strain of Aspergillus niger was characterised by a slightly more intensive absorption within its entire spectrum range in comparison to control strain. In case of Aspergillus flavus the absorption was higher for control strain.

12

Channeling of vanadium in two species of fungi

84%

Abu-Seidah A. A.

Acta Microbiologica Polonica

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1998

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tom 47

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nr 4

13

Inhibitory effects of ammonia solution on growth and aflatoxins production by Aspergillus parasiticus NRRL-2999

84%

Namazi M., Allameh A., Aminshahidi M., Nohee A., Malekzadeh F.

Acta Poloniae Toxicologica

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2002

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tom 10

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nr 1

14

Inactivation of fungi in vitro by photosensitization: preliminary results

84%

Luksiene Z., Peciulyte D., Lugauskas A.

Annals of Agricultural and Environmental Medicine

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2004

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tom 11

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nr 2

Photosensitization is based on the interaction of 2 completely non-toxic agents - a photosensitizer, accumulated in microorganisms, and visible light. This interaction induces radical-based cytotoxic reactions in the presence of oxygen. The photosensitization phenomenon is widely involved in the treatment of tumors in oncology, in curing arthritis and atherosclerosis. In this work, the possibility to inactivate pathogenic and harmful fungi by photosensitization is shown. A new treatment methodology is proposed on the basis of effective inactivation of the several micromycetes, such as Aspergillus flavus, Trichothecium roseum, Fusarium avenaceum, Rhizopus oryzae, by photosensitization.

15

Infuence of aflatoxin present in forages and concentrated feedingstuffs on milk and some serum biochemical parameters in goats

84%

Bingol N. T., Tanritanir P., Dede S., Ceylan E.

Bulletin of the Veterinary Institute in Puławy

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2007

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tom 51

|

nr 1

Eight private farms as groups were used in the study. Each group contained randomly selected ten goats. These animals were fed forage and concentrated feed. Serum glucose, total protein, albumin, globulin, cholesterol, and triglyceride levels, and ALP (alkaline phosphatase), ALT (alanine -amino transferase), AST (aspartate amino transferase), GGT (γ- glutamyl transferase), and LDH (lactate dehydrogenase) activities were analysed. There were no correlations between glucose, ALP, AST, GGT and feed total aflatoxin (AF) concentrations. There were positive correlations between feed AF and LDH activities (P<0.01), between feed AF and milk aflatoxin M1 (AFM1) (P<0.01). On the other hand, there was a negative correlation (P<0.01) between feed AF and total protein levels were also present. There was negative correlation between ALT concentration (P<0.05) and AF in feed. There was negative correlation between concentrations of albumin and globulin (P<0.01) and positive correlation between triglyceride concentration (P<0.05) and AF level in feed. It was noticed that a marked increase in the level of AFM1 in milk due to an increase in total aflatoxin levels in feeds (P<0.01).

16

Właściwości szczepów Aspergillus flavus LINK izolowanych z gleb i roślin uprawnych południowej Polski. Część II. Właściwości fitotoksyczne i zootoksyczne metabolitów

83%

Chmiel M. J.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2011

|

tom 567

W pracy oceniano zdolność do produkcji fitotoksycznych i zootoksycznych metabolitów przez wyizolowane ze środowiska rolniczego szczepy Aspergillus flavus. Testowane szczepy pochodziły z gleby i roślin uprawnych. Badano wrażliwość nasion grochu i rzepaku oraz larw Artemia salina na metabolity zawarte w płynach pohodowlanych. Badane izolaty A. flavus charakteryzowała bardzo zróżnicowana zdolność do produkcji toksycznych metabolitów. Tylko trzy z przebadanych szczepów nie wytwarzały substancji o charakterze fitotoksycznym. Badania zootoksycznych właściwości produktów metabolizmu badanych szczepów potwierdziły wyniki uzyskane w testach fitotoksyczności. Cztery szczepy pochodzące z roślin sklasyfikowano jako silnie toksynotwórcze. Badane szczepy produkowały metabolity o różnej toksyczności niezależnie od źródła pochodzenia A. flavus.

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Plesnie i ich toksyny a bezpieczenstwo zywnosci

71%

Popowski J.

Żywność Żywienie Prawo a Zdrowie

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2000

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tom 09

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nr 1

109-114

18

Ocena ryzyka przy pracach budowlanych. Część 9: Czynniki biologiczne - identyfikacja zagrożeń i ocena ryzyka

67%

Bozejewicz E.

Atest

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2014

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nr 04

19

Further studies of extracellular enzyme profiles of xerophilic fungi isolates from dried medicinal plants

67%

Janda K., Ulfig K., Markowska-Szczupak A.

Polish Journal of Environmental Studies

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2009

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tom 18

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nr 4

627-633

The contamination of dried medicinal plants with microscopic fungi has been the subject of many studies. However, no data on extracellular enzyme activities of xerophilic fungi contaminating the plants have been found in the literature. Therefore, the objective of our study was to determine extracellular enzyme profiles of fast-growing xerophilic fungi, i.e. Aspergillus flavus, A. fumigatus, A. melleus, A. nidulans, A. niger, A. parasiticus and Trichothecium roseum isolated from dried medicinal plants from herbal shops in Szczecin, Poland. Solid media and the API ZYM® test were used for measuring enzyme activities. Among the fungi, A. melleus had the highest hydrolytic activity on milk, gelatin, starch, tributyrin, rapeseed oil and biodiesel oil agars, while A. fumigatus showed extremely high stimulation index values on rapeseed oil and biodiesel oil agars. The stimulation index increased during a 5-day incubation period. In the API ZYM® test A. nidulans showed the highest hydrolase activity. Among the hydrolases, ß-glucosidase activity was the highest, followed by acid phosphatase, N-acetyl-ß-glucosaminidase and naphthol-AS-BI-phosphohydrolase activities. The fungi contaminating dried medicinal plants are able to utilize a number of substrates and, therefore, possess high biodeterioration potential. Due to the ability to degrade hydrocarbons, fungal isolates from dried medicinal plants can be used for biotechnological purposes, e.g. in air biofiltration and waste or soil bioremediation.

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Prevalence of aflatoxin B1 contamination in pre- and post-harvest maize kernels, food products, poultry and livestock feeds in Tamil Nadu, India

67%

Vijayasamundeeswari A., Mohankumar M., Karthikeyan M., Vijayanandraj S., Paranidharan V., Velazhahan R.

Journal of Plant Protection Research

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2009

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tom 49

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nr 2

221-224

Aflatoxins, a group of mycotoxins mainly produced by Aspergillus flavus and A. parasiticus, have adverse health effects on humans and livestock that ingest aflatoxin- contaminated food products and feeds. To secure the safety of food and feed, regular monitoring of aflatoxin levels is necessary. In order to understand the magnitude of aflatoxin contamination, a survey was conducted in different agro-ecological zones of Tamil Nadu, India and 242 samples consisting of pre- and post-harvest maize kernels, food products, poultry and livestock feeds were collected from farmers' fields, poultry farms, retail shops and supermarkets and analyzed for aflatoxin B1 (AFB1) contamination by enzyme- linked immunosorbent assay (ELISA) using antiserum raised against aflatoxin B1-Bovine serum albumin (AFB1-BSA). The results indicated that 61.3% of the maize kernel samples were contaminated with AFB1 and the levels of AFB1 in 26% of the pre- and post-harvest maize kernels exceeded 20 μg/kg. The highest level of AFB1 (245 μg/kg) was recorded in post-harvest maize kernel samples. In food products AFB1 was detected only in two samples out of 30 samples tested. Furthermore, the levels ranged from 0.6 to 3.7 μg/kg. In poultry feeds, AFB1 was detected in 30 out of 53 samples and the levels ranged from 0.7 to 31.6 μg/kg. Among the 40 livestock feed samples evaluated 29 samples were contaminated with AFB1 at level ranging from 1.8 to 244.9 μg/kg.

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