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It is a common opinion that main challenge for plant production is to cope with environmental stresses, both abiotic and biotic. Although biostimulator Asahi SL has been used for years to improve plant status and obtain possibly the highest and best quality yield, especially under conditions unfavourable for plant cultivation, its mode of action is still not understood. In this work the effect of Asahi SL on Arabidopsis thaliana plants grown under drought stress was studied. Plants grown under drought stress conditions and treated with Asahi SL were, as compared to untreated, higher and more advanced in development, particularly generative. Biomass accumulation was stimulated by biostimulator mainly due to better photosynthetic apparatus efficiency manifested by a higher (i) assimilation area, (ii) chlorophyll content, (iii) intensity of photosynthesis and (iv) improvement of chlorophyll a fluorescence parameters. Asahi SL treated plants were characterized by a lower concentration of biological active ABA. Despite the higher transpiration and lowered stomatal resistance RWC and water content were unchanged in Asahi SL treated plants which can be explained by increased water uptake in biostimulator sprayed plants. The obtained results clearly showed that the application of biostimulator played a protective role against drought stress.
Within a three-year cycle of studies, experiments were conducted with Polyversum WP biofungicide and Asahi SL biostimulant used as crop protection products of strawberry fruits of Kent and Senga Sengana varieties. A comparative combination was made of the following fungicides: thiram, iprodione, pyrimethanil and fenhexamid, that were applied accordingly to the strawberry protection program. All protective treatments had no significant effect on dry matter content of the fruits of both strawberry varieties and on the marketable yield in the case of Kent var. fruits. The use of combination of Asahi SL and Polyversum WP preparations resulted in increased contents of vitamin C and total sugars in fruits of Kent var. In the case of Senga Segana var., this treatment had a beneficial effect only on vitamin C content in one year of the study and on the increase in the marketable yield when coupled with synthetic fungicides. The applied crop protection products caused also sporadic changes in the contents of extract, organic acids, polyphenols, anthocyanins and antioxidative capacity of fruits of both strawberry varieties.
In pot experiments on cucumber cv. Śremski F1, the effect of short-term chilling on plants earlier treated with triacontanol (TRIA) and Asahi SL was investigated. These plants were grown in a phytotron at an air temperature of 27/22ºC (day/night), using fluorescent light with far flux density of 220 μmol x m-2 x s-1, with a photoperiod 16/8. At the 4th true leaf stage, the respective experimental series were sprayed with: 1) H2O – control, 2) TRIA 0.01, 3) TRIA 0.1, 4) TRIA 1.0 mg x dm-3, 5) Asahi SL 0.2, 6) Asahi SL 0.3%. After 24 hours one half of the plants from each experimental series was treated for a period of 3 days at a temperature of 12/6ºC, with all the other growth conditions unchanged. The obtained results have shown that short-term chilling stress caused a significant increase in electrolyte leakage, free proline content and in the activity of guaiacol peroxidase in leaves, but a decrease in chlorophyll a+b content, stomatal conductance, transpiration, photosynthesis, leaf area and in the activity of catalase in leaves. The application of TRIA or ASAHI SL on leaves in the pre-stress period reduced the values of the traits which had been increased as a result of chilling and increased those which had reduced. Generally, TRIA was most effective at a concentration of 0.1 mg x dm-3, and Asahi SL at a concentration of 0.3%.
W latach 2002-2004 przeprowadzono doświadczenie polowe, w którym badano wpływ preparatu stymulującego wzrost Asahi SL na jakość krzewów róż odmiany 'Flamingo'. Preparat stosowano w formie oprysku w czterech stężeniach 0,1; 0,2; 0,4 i 0,6%. Asahi SL nanoszono na rośliny 4 i 6-krotnie w czasie wegetacji. Asahi SL stosowany w stężeniach od 0,1 do 0,4% wpływał korzystnie na masę krzewu i liczbę pędów pierwszego i drugiego rzędu. Zwiększona częstotliwość oprysku roślin z 4 do 8 w okresie wegetacji nie miała istotnego wpływu na jakość krzewów róż odmiany 'Flamingo'.
In pot experiments conducted on cucumber cv. Śremski F1, the effect was studied of short-term chilling stress on plants which had grown from seeds germinating in the solution of Asahi SL or treated with this biostimulator during the early growth period. The plants were grown in a phytotron at an air temperature of 27/22ºC (day/night), using fluorescent light with FAR flux density of 220 μmol x m-2 x s-1 and with a photoperiod of 16/8. The biostimulator was applied using the following methods: a) germination of seeds in 0.01% and 0.05% solution, b) watering of plants twice with 0.01% or 0.05% solution, c) spraying leaves with 0.3% or 0.5% solution. Plants sprayed with distilled water were the control. After 24 hours from foliar or root application of Asahi SL, one half of the plants from each experimental series was treated for a period of 3 days at a temperature of 12/6ºC, with all the other growth conditions unchanged. The obtained results show that short-term chilling stress caused a significant increase in electrolyte leakage, free proline content and in the activity of ascorbate peroxidase in leaves, but a decrease in the content of chlorophyll, its maximum fluorescence (Fm) and quantum yield (Fv/Fm), carotenoid content, stomatal conductance, transpiration, photosynthesis, leaf biomass and in the activity of catalase in leaves. Foliar or root application of Asahi SL in the pre-stress period decreased the values of the traits which increased as a result of chilling or increased those which decreased. Higher concentrations of the biostimulator solutions, applied using this method, were more effective. The application of the biostimulator during seed germination did not result in significant changes in the response of plants to chilling stress.
Chamomile is a valued medicinal plant of high economic importance and therefore agronomic practices in growing this plant are continually improved. One of methods to improve the quantitative and qualitative parameters of herbal raw material can be the application of foliar sprays (growth stimulators, foliar fertilizers, Effective Microorganisms). Row spacing, which has an effect on the use of fertilizers by the plant and natural habitat conditions, is of great importance in chamomile growing practices. During the period 2011–2013, a field experiment was conducted, the aim of which was to determine the effects of selected foliar-applied sprays and different row spacings on the yield and quality of chamomile cv. ‘Złoty Łan’ raw material. The study included three formulations: Asahi SL, Ekolist P, and EM Farming. Plots without the application of these foliar sprays were the control treatment. The other factor included in the experiment was row spacing, which was as follows: 25, 35, and 45 cm. Plant height and number of inflorescences per stem, total yield of raw material and the content of essential oil and flavonoids in raw material were determined. The growth stimulator Asahi SL was proven to positively affect the yield and quality of chamomile raw material. Ekolist P had a lower effect on the improvement in the parameters analyzed. The reports of some authors that Effective Microorganisms (EM Farming) had no effect whatsoever on plant productivity were confirmed in this study. It was shown that growing chamomile in rows 45 cm apart, or at a row spacing of 35 cm, was most beneficial for the yield and quality of chamomile raw material. A narrow row spacing (25 cm) contributed to a decrease in yield and deterioration in its quality.
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Asahi SL stimulates plant’s vital processes like growth and development, affects physiology and biochemistry, what often leads to increased biomass accumulation and yield. However, common is opinion that application of this preparation could be beneficial only, when treated plants are grown under unfavorable conditions. Therefore the aim of this work was the assessment of the stimulatory effect of Asahi SL on Arabidopsis thaliana L. and ornamental amaranth plants grown under optimal conditions. Plants treated with Asahi SL were higher and more advanced in development, particularly generative. Biomass accumulation was greater after biostimulator application mainly due to better photosynthetic apparatus efficiency, which was manifested by (i) greater leaf area, (ii) higher total chlorophyll content and (iii) increased intensity of photosynthesis. Effect of Asahi SL on chlorophyll a fluorescence was marginal. Despite of higher transpiration and lowered stomatal resistance the RWC was almost unchanged in biostimulator treated plants what was attributed to increased water uptake. Obtained results clearly showed that Asahi SL applied on plants can also be effective and beneficial when they are grown under optimal conditions.
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