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To assess seroprevalence of Neospora spp. in asymptomatic horses in Ankara, Turkey, 19 mares and 56 stallions older than 2 years of age were examined using ELISA; 9.3% of the horses were seropositive. Seroprevalence of N. caninum in mares was twice of that in stallions (15.8 vs 7.1%) and all appeared to be asymptomatic.
Acanthamoeba keratitis is a blinding infection that is becoming increasingly important in human health. Early diagnosis is a prerequisite for successful treatment and requires identification of Acanthamoeba at the genotypic level. The genus Acanthamoeba consists of both pathogenic and non-pathogenic species and has been recently classified into 13 different genotypes, T1-T12 and T14. More importantly, 95% of Acanthamoeba isolates that produce keratitis belong to T4 genotypes. In this study, we attempted to determine whether predominance of T4 isolates in Acanthamoeba keratitis is due to greater virulence or greater prevalence. We isolated 18 Acanthamoeba isolates from environmental samples in Ankara, Turkey and determined their pathogenic potential by means osmotolerance, temperature tolerance and in vitro cytotoxicity assays using corneal epithelial cells. Ribosomal DNA sequencing revealed that 10 isolates belong to T2, 5 belong to T3,2 belong to T4 and one belongs to T7 genotype. As expected, T3 and T4 isolates exhibited the most pathogenic traits and were osmotolerant, temperature tolerant and exhibited severe corneal epithelial cell cytotoxicity indicating their pathogenic potential. Overall these data indicate that high frequency of T4 isolates in keratitis cases may well be due to their greater virulence. This is the first report presenting environmental distribution of Acanthamoeba in Ankara, Turkey.
To detect aflatoxin (AF) or ochratoxin A (OTA) contamination, 25 retail ground samples of 12 different types of seed-, pulses-, and cereal-flours and starches were randomly collected from markets and traditional bazaars in Ankara, Turkey. The levels of AF in the retail ground samples were determined by high performance liquid chromatography (HPLC) and ranged from 0.03-3.16 ppb. The percentage of contaminated samples for aflatoxin B1, B2, G1, and G2 were 64, 60, 72, and 76%, respectively. The determination of OTA level was performed by enzyme-linked immunosorbent assay (ELISA), and they were ranged between 0.27-4.07 ppb (n=24). However, the screened mycotoxin levels in the samples were under the permission limits of Turkey, the daily intake of these products corresponds to at least 50% of daily diet in our country. Routine measurements of the toxin levels in foods and feeds should be carried out to prevent their harmful effects on health.
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