Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 35

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  Anisakis simplex
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
Quantitative indices of herring infestation in Sakhalin waters reveal a high infection rate of fish in the basin of the Sea of Okhotsk, off the east coast of the island. Moreover, in certain years almost 100% of herring were infested. The so-called local populations as well as widely migrating Sakhalin Hokkaido herring are confined to the area of this waterbody. In the basin of the Sea of Japan, off the west coast of Sakhalin, the indicator values of herring infestation are much lower. The local population as well as widely migrating herring of Sakhalin Hokkaido population are confined to this marine area. The causality analysis of herring infestation in these areas allows to confirm our earlier conclusions about population structure of herring inhabiting the waters around Sakhalin Island.
The aim of this paper is to establish the haematological and organic changes in halothane-sensitive and halothane-resistant pigs in the course of experimental anisakiosis. Experiments were carried out on two groups of pigs (3 animals each). The pigs from the first group were given fifty A. simplex B larvae, the pigs from the second one received ten larvae and then again fifteen larvae each after the 5th and 6th days. The number of leucocytes, neutrophiles, lymphocytes, monocytes and eosinophiles was greater - different in both series, but similar in halothane (stress) - resistant and - sensitive pigs. In the case of sensitive pigs much greater reactive changes were found in the stomach submucosa than in that of resistant pigs. In this group of pigs nematode larvae have also been traced in the submucosa of the same organ.
α-Amylase is present in the third (L3) and in the fourth-stage (L4) of larvae from Anisakis simplex. The enzymes from both sources differ in same of their properties. Α-Amylase from L3 showed a maximum at pH 7,8, enzyme from L4 stage at pH 6,5. The α-amylase from L3 was mainly lysosomal enzyme. The enzyme from L4 was located in the microsomal fraction. The L3 α-amylase showed the inhibition by EDT A and by -SH reagent iodoacetic acid. These agents did not change the activity of L4 enzyme. Bath izoenzymes were unaffected by calcium and magnesium ions. Generally the α-amylase from L4 stage had higher activity (3,71 u/mg) than L3 one (2,29 u/mg).
The studies were carried out on guinea pig males. The animals were infected with 30 larvae (L3) of Anisakis simplex. After 6 hrs of invasion the animals were dissected. In homogenized pancreas and duodenal contents activities of trypsin were determined. In stomach content activities of pepsin were determined. The activities of trypsin in duodenal contents and in pancreas homogenate from infected animals were lower in comparison with the control animals. The activities of pepsin were higher in infected animals.
Geographical distribution of Baltic herring (Clupea harengus membras (L., 1761)) affected with externally visible pathological symptoms is demonstrated, based on the observations of 5 028 individuals. The fish were collected in period 25.11-08.12.1994, from 36 sampling hauls performed on a transect from the south-western to the north-eastern Baltic Sea. The results of analyses indicate statistically significant differences in the fraction of herring with externally visible diseases (mean percentage of 3.7%) dependent on the geographical area. A relationship between the prevalence of emaciation (mean prevalence of 3.3%), infection (mean prevalence of 3.7%) in herring with the parasitic larvae of Anisakis simplex (Rudolphi, 1809), and the area of sample collection was shown to exist.
Live Anisakis simplex third-stage larvae (L3) penetrate gastrointestinal mucosa after they are ingested in raw or undercooked seafood, thereafter causing gastrointestinal manifestations and allergic manifestations such as urticaria and anaphylaxis. These allergic reactions are mediated by specific IgE to L3 allergens, especially excretory-secretory (ES) allergens. Recent evidences suggest that only live larvae can cause allergic reactions, although cases attributable to ingestion of cooked, frozen seafood have been reported. Therefore the risk of Anisakis-associated hypersensitivity by ingestion of properly cooked and frozen fish remains controversial. No prior report describes the kinetics of antibody production in experimental animals after oral inoculation with dead L3. This study used ELISA to assess antibody production in rats inoculated orally with dead L3. Positive absorbance value in IgG, IgM, and IgE specific to ES antigen from L3 were found in rats inoculated with live L3 but not with dead L3 (frozen, heated, cut, or homogenized). At one week post re-inoculation with live or frozen L3 to the initially sensitized rats, the absorbance value of the specific IgM and IgE to ES antigen elevated quickly and highly in rats that had been re-inoculated with live L3, but they decreased slightly or did not change in rats inoculated with frozen L3. These results suggest that only ingestion of live L3 can produce the specific antibody and induce initial and secondary sensitizations to L3.
The cultivation was done on Eagle's media as well as Eagle's media enriched with the supplement of 10 and 20% of bovine serum. In the media without serum the larvae lived significantly shorter and did not get through molting. They lived the longest and came through molting the fastest in the media with pH 2.0 supplemented with 20% SB. In the media with the pH 4,0 and 7.3 the larvae lived significantly shorter and only a small proportion reached stage IV.
Of the 400 pike-perches examined, 12% were infected with L3 Anisakis simplex. The infected fish were over 45 cm long. The hypothesis submitted was that the pike-perches may constitute the source of infection in accidental hosts, i.e. in man.
The larvae of Anisakis simplex had the largest influence upon decreasing the activity of porcine pepsin. The activity of that enzyme in tests, where the larvae were present during the entire period of incubation, was lower than in the controls. No similar trends were observed in case of the solutions with bovine and porcine trypsin. The activity of those enzymes in the solutions containing the larvae was higher than in the controls. Only the activity of porcine trypsin after 10 h of incubation was slightly lower in the experimental sample than in the control, however, during the later hours the dynamics of the activity decrease of that enzyme in the controls was higher than in the experimental samples. The recorded activity of papain in the samples containing the larvae was higher than that in the controls during the entire time of the experiment.
Background. Proteolytic enzymes may serve multiple functions: they may inhibit the host′s blood clotting, protect the parasite from the host ′s immune response, facilitate parasite′s migration within a tissue by decomposing the tissue barrier, enhance the hatching and moulting of larvae, and play an important role in their feeding. Learning their identity leads to a better understanding of a host-parasite system. The objective of this study was to check, using biochemical methods, if, in addition to proteases, ES products and extracts of 3rd and 4th larval stages of Anisakis simplex (Rudolphi, 1809) contain other hydrolases. Materials and Methods. Stage-3 larvae (L 3 ) of A.simplex were removed from Baltic herring, Clupea harengus membras Linnaeus, 1761 caught in the Baltic Sea. Stage-4 larvae (L 4) were obtained from an L 3 stage culture kept in Eagle ′s medium. The solutions containing ES products were collected and dialysed at 4°C against distilled water for 24 h. Larval extracts were obtained by homogenising the larvae in a physiological saline (0.9 % NaCl) solution in a glass homogeniser. The homogenate was centrifuged for 10 min at 3000 G. The supernatant was used in enzyme activity assays. Enzymatic activity of ES products and homogenates of L 3 and L 4 larvae of A. simplex was determined with the API ZYM test. Results. The excretion-secretion product of L 3 and L 4 larvae of A. simplex revealed activities of 10-and 11 hydrolases, respectively. Activity of esterase, esterase lipase, valine arylamidase, and N-acetyl-ß-glucosaminidase in the L 4 larvae extracts was higher than the activity of a corresponding enzyme assayed in the L 3 extracts. Only in the case of acid phosphatase, its activity in L 3 ES products was twice that of the activity found in ES products of L 4 larvae. Enzymes such as trypsin, chymotrypsin, and ß-glucosidase were not detected in extracts from L 3 larvae. Conclusion. Activities of most hydrolases in the L 4 extracts were higher than the activities of corresponding enzymes assayed in the L 3 extracts. The high activity of these enzymes found in L 4 larval extracts could be related to a different feeding mechanism of stage-4 larvae.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.