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155 rhizosphere soil and root mixtures were collected from under Ammophila arenaria colonizing maritime dunes of the island Bornholm (Denmark) to determine arbuscular mycorrhizal fungi (AMF) of the phylum Glomeromycota co-existing with this plant. In the laboratory, each mixture was divided into two parts. One part was used to establish a pot culture with Plantago lanceolata as the host plant to initiate sporulation of fungi that had not produced spores in field conditions. In the second part, the numerical and species composition of the spore populations of AMF sporulating in the field was determined. Spores of AMF were found in 70 fieldcollected samples and 134 trap cultures. They represented 26 species and six undescribed morphotypes in six genera of the Glomeromycota. Of them, 20 species and three morphotypes in five genera occurred in the field, and 16 species and three morphotypes in five genera were found in trap cultures. The fungi most frequently revealed were members of the genus Glomus; a total of 17 species and six morphotypes of this genus were recognized. Considering the occurrence of spores in both field samples and trap cultures, the fungi most frequently co-occurring with roots of A. arenaria growing in the dunes of Bornholm were G. irregulare (present in 73.6% of samples), followed by Scutellospora dipurpurescens (19.4%) and Archaeospora trappei (10.3%). However, Glomus irregulare mainly sporulated in trap cultures; spores of this fungus were found in only 0.6% of field samples. Other relatively frequently found species were G. aggregatum (9.0%), G. eburneum (7.1%), Paraglomus laccatum (5.2%), and S. armeniaca (6.5%). The species most abundantly sporulating in the field were G. aggregatum (produced 28.36% of all spores isolated), G. badium (11.00%), and S. dipurpurescens (21.55%).
A root-lesion nematode, Pratylenchus brzeskii sp. nov., is described and illustrated from the roots of Ammophila arenaria (L.) Link from the coastal dunes of the Netherlands; this new species is morphologically characterized by; relatively long females (625-735 µm) with two lip annuli, long stylet (± 19 µm ) with broad stylet knobs, anteriorly slightly indented, short DGO; pharyngeal gland lobe ranging from 65 to 100 µm; four lateral lines present, inner lines closely together; spermatheca faint, oval to rectangular shaped; vulva posterior, ranging from 75 to 78 %, lips slightly protruding, posterior uterine sac 19 to 35 pm long; tail conoid, terminus smooth, rounded to narrowly rounded, relatively long hyaline tail part present. Males occurred abundantly and are slightly smaller in most morphological characteristics. PCR was used to amplify the internal transcribed spacer regions of a ribosomal DNA-cluster of P. brzeskii sp. nov. and compared with the morphological related species P. coffeae, P. loosi and P. penetrans. The rDNA fragments were cut with restriction enzymes; interspecific RFLP’s were observed. Pratylenchus brzeskii sp. nov. was also isolated from the coastal dune grasses Elymus farctus (Viv.) Meldris and Leymus arenarias Hochst., and detected in coastal dunes of Belgium, France and Poland.
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