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In order to clarify the relationship between American martens Martes americana (Turton, 1806), and roads in boreal forests, we tested the effect of distance from road on marten habitat use by comparing the frequency of occurrence, and the number of, marten snow tracks in 27 pairs of 300 and 400 m transect segments perpendicular to access roads, and corresponding segments 800 or 1000 m away from the road, for a total of four spatial analyses. The number of black spruce Picea mariana, stems was generally lower near roads (—94.0 < Ts < —63.0, p < 0.05), but otherwise forest composition generally did not differ significantly between road and forest segments. Depending on the spatial scale, 11-14 road and 15 - 18 forest (out of 27) segments had marten tracks, but the difference was not statistically significant (Fisher's exact test, p > 0.05). In all analyses, marten tracks were significantly fewer near roads (Wilcoxon test: -74.0 < Ts < -62.0, p < 0.01) than away from roads. In segments with tracks, track density was significantly lower near roads than away from roads in three of four spatial analyses (-3.3 < Ts < -2.9, p < 0.01). The findings suggest that snow track samples near roads can detect marten occurrence, but do not reflect the clumping pattern observed away from roads. Habitat selection studies using tracks should consider the possible effect of roads on marten movement patterns.
Prey occurrence from stomach and fecal samples were compared in American martenMartes americana Turton, 1806, and fisherM. pennanti Erxleben, 1777. Diets were analyzed from stomach and fecal contents in four sex-age marten groups and four fisher groups, and dietary differences tested between species, sex, age and sample sources. Prey richness was compared between the two sample sources. Relative occurrence of dominant or co-dominant prey items did not differ between stomach and fecal samples in any of the groups. However, both series revealed inter-specific contrasts. Binomial correlations of prey occurrence between sample sources were significantly negative for most prey items. Correlations of prey richness between samples sources were not significant except in larger marten sample. Our results failed to reveal a bias in using fecal sample source. Thus we suggest that diet analyses using either sample source are valid when diets are assessed in a relatively large number of animals.
We examined sequence variation in mitochondrial and nuclear genes of seven species of the genus Martes (Mustelidae, Carnivora): Martes americana (American marten), Martes flavigula (yellow-throated marten), Martes foina (beech marten), Martes martes (pine marten), Martes melampus (Japanese marten), Martes pennanti (fisher) and Martes zibellina (sable), focusing on the phylogenetic history of the Hokkaido subspecies of the sable, Martes zibellina brachyura. Nucleotide sequence analysis of the mitochondrial cytochrome b gene confirmed the view that the Hokkaido sable population has lower genetic diversity. In contrast, network analysis of a nuclear gene related to coat colour, melanocortin-1 receptor (Mc1r), revealed two different haplogroups for this population: one shared with that of Russian sables and the other specific to this population but with a close relationship with the American and Japanese martens, implying that these endemic haplotypes are composed of uncharacterised ancestral lineages of a past population. We also examined the sequence variation in a neighbouring nuclear gene, transcription factor 25 (Tcf25), located about 5 kb upstream from the Mc1r gene, and found similar trends. The sable genome leaves the impression that Hokkaido hosted ancient marten lineages, with subsequent recent migrations from the continent. The validity of a candidate Mc1r mutation for the entirely yellow coat observed on Hokkaido sables was also discussed.
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