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In a field experiment conducted in 1998-2002 on sandy-loam soil the effect of applying chosen growth regulators in an American ginseng culture was examined. In the second year of vegetation (June 2000) plants were sprayed with following substances (each in a dose of 100 and 200 mg/1): kinetin, daminozide, mixture (1:1) of gibberellic acid (GAj) with potassium salt of a-naphtyl acetic acid - KNAA and IPO-1 (benzimidazole derivative) - a new preparation obtained from Institute of Organic Industry in Warsaw. Amongst the applied growth regulators positive effect on growth of plants and yield of roots, as well as on the content of active compounds had IPO-1, daminozide (in a dose of 100-200 mg/1) and, to a certain extent, kinetin (200 mg/1), while the mixture of GAj and KNAA affected the above-mentioned features negatively.
Field studies on the health of American ginseng cultivated in the Lublin district on poor sandy soil were conducted in the years 2004-2006. The studies involved treatment combinations with irrigation and without irrigation as well as foliar fertilization with Alkalin PK and Resistim of American ginseng plants. Mycological analysis was made of diseased ginseng parts with the aim of determining the quantitative and qualitative composition of fungi-like organisms and fungi threatening the cultivation of this plant. Fungi from the genera of Cylindrocarpon, Fusarium and the following species Alternaria alternata, , Rhizoctonia solani, Sclerotinia sclerotiorum, as well as fungi-like organisms: Pythium irregulare and Phytophthora sp., were isolated from the infected parts of ginseng. The smallest number of fungi was isolated from the plants growing on the plots without irrigation and those where foliar application with Alkalin PK was applied.
The influence of various soil materials (sandy, silty and compost) on growth and develop­ment of blueweed (Echium plantagineum L.) was examined in pot experiment carried out in 2001-2003. Seeds were sown at the beginning of April and at the beginning of May. Plant growth and the weight of produced seeds depended on the soil material type. The high­est vegetative mass as well as seeds was obtained on compost. However, the vegetation period and seed maturation were prolonged on that kind of soil. Among compared sowing dates, April was more favorable - the plants were taller, more branched and produced big­ger aboveground parts, roots and seed weight than those from May sowing. Furthermore, delayed sowing caused decrease of fat content in the seeds and a slight increase of GLA and SDA levels. Blueweed growing on sandy material accumulated the most of fat in seeds. The fat content decreased along with the increase of soil abundance in nutrients. The per­centage of GLA and SDA in oil was similar and did not depend on soil type significantly.
In the years 2004–2006 on American ginseng plantation covered with the mineral mulch Agran, located in Krasnystaw (the Lublin region) there were compared the effects of five methods of plant protection. Each year 5 plants showing disease symptoms were sampled from particular experimental combination in order to perform the mycological analysis. The results of the analysis showed that in particular years of the experiment the quantitative and qualitative composition of pathogenic fungi isolated from the examined ginseng parts was differentiated. There was proved that fungi from the genera of Altenaria, Fusarium, Cylindrocarpon, Phytophthora and Pythium irregulare, Rhizoctonia solani Botrytis cinerea create a considerable danger for the ginseng plants.
The aim of this paper was to investigate the chemical composition of leaves and roots of American ginseng (Panax quinquefolium L.) and to estimate the antimicrobial and anti­oxidant activity of ginseng extracts. Investigations showed that ginsenosides and phenolic compounds (flavonoids, phenolic acids and tannins) are present in both investigated raw materials. Ginseng leaves are characterised, in comparison to ginseng roots, by higher concentrations of ginsenosides, flavonoids and phenolic acids. The tannin fraction is pres­ent only in ginseng roots. Preliminary investigations on microbiological activity showed that both examined ginseng extracts from Panax quinquefolium had weak activity against Gram-negative and Gram-positive organisms as well as weak antifungal activity. Studies on antioxidant activity of ginseng extracts showed that leaf extract had about 30% stronger antioxidant activity in comparison to a root extract.
A field experiment was conducted in 2002-2006 to investigate the severity of weed in­festation and roots yields in relation to weed management methods in American ginseng (Panax qumquefolium L.) cultivation. Hand-made weeding was compared to various her­bicides treatments. Herbicides application in American ginseng cultivation significantly limited species composition and number of weeds per 1 m2. The effects of application of 2.4 D and dicamba combined with fluazifop-p-butyl were superior to those of hand-made weeding, whereas other preparations (propyzamide and fluazifop-p-butyl, linuron and flu- azifop-p-butyl as well as prometryn and fluazifop-p-butyl) damaged ginseng plants which finally led to decrease of raw material.
American ginseng belongs to the Araliaceae family and, along with Asian gin­seng (Panax ginseng), traditionally its roots have been used for a wide range of beneficial pharmacological effects [1], Its commercial cultivation has been con­ducted over the last century in order to supply worldwide demand and due to depletion of wild ginseng.
We carried out histochemical studies to find the localization of ginsenosides in roots of Panax quinquefolium cultivated in Poland. We performed an anatomical study on the structure and localization of secretory canals on the cross section of 4-year-old American ginseng roots. We observed the occurrence of large secretory canals, mainly in the middle part of the secondary cortex and less in the phloem layer. In our studies, moreover, we demonstrated the production of secretory canals within the periderm layer. After the anatomical study, the 4-year-old ginseng root was divided into periderm, cortex and xylem, and the ginsenosides were extracted from each part of the root. The TLC separation of ginsenosides was performed on silica gel Si60 glass plates with chloroform-methanol-ethyl acetate-water-hexane, 20+22+60+8+4 (v/v) as mobile phase. Quantitative analysis of ginsenosides was performed by using the TLC-densitometric method. Concerning the distribution of ginsenosides in the different anatomical parts of the root of Panax quinquefolium, they were contained in the periderm layer at the highest level.
The investigations were carried out in 2003-2004 on American ginseng (Panax quinquefolium L.) plantation localised in the mixed forest in Trzciniec (Lubelski province). The object of the study was the soil from ginseng culture protected by the biological and chemical method. In the experiment there was also control plot - without any protection during plants vegetation. There were also analysed the infected roots of ginseng plants. Microbiological analyses showed that soil from control plots was characterised by the smallest average total number of bacteria. In 1 g of dry weight of soil after Polyversum application there was stated the highest number of bacterial colonies from Bacillus and Pseudomonas genus. The highest number of fungal colonies was obtained in 1 g of dry weight of soil on the control plots. The highest number of saprophytic fungi (including those with antagonistic character) was obtained from the soil where the biological plant protection was applied. Generally, independently of a plant protection method, American ginseng plants were affected by Alternaria alternata, Cylindrocarpon spp., Fusarium spp., Pythium irreguläre and Rhizoctonia solani.
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