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Common ragweed (Ambrosia artemisiifolia L.) is the most frequent weed in the Carpathian Basin and is spreading fast in other parts of Europe. In recent years, besides the wild type, a mutant genotype resistant to atrazine herbicides has evolved and is now widespread in many areas. The present study demonstrates that the atrazine resistance of ragweed is maternally inherited, and is caused by a point mutation in the psbA chloroplast gene. The promoter 5'-untranslated region and the open reading frame regions of the gene were analysed, and a homology search was performed. Both the atrazine-resistant and susceptible types of cpDNA were present in atrazine-resistant plants, while the mixed presence of both genotypes in the same plant, known as heteroplasmy, was not unequivocally detectable in susceptible plants.
In the study, the biology of fl owering of Ambrosia artemisiifolia L. was investigated and the pattern of the Ambrosia pollen seasons in Lublin in the years 2001-2008 was characterised. The structure of male and female A. artemisiifolia fl owers was observed in cultivated plants under controlled conditions in the 2000 vegetative season. The number of pollen grains produced by the stamen, fl ower, infl orescence and plant was determined. It was shown that in A. artemisiifolia fl owers nonfunctional pistils occurred with a reduced ovary, performing the role of a pollen presenter. The pistils found in female fl owers differed signifi cantly in their morphological features from the pistils in male fl owers. It was calculated that one stamrn produced an average of 3 375 pollen grains, whereas one fl ower 16 875. A plant which produces 20 racemes may release over 420 million pollen grains into the atmosphere. The Ambrosia pollen seasons in particular years had different patterns. In some years, the days of maximum concentration were in the second half of August, in other years in the fi rst half of September. Over the 8-year period, the maximum daily concentration had a mean value of 116 pollen grains in m3 (31-311). Annual total concentrations of Ambrosia pollen grains ranged between 194 and 1200 grains, and it was 523 grains on the average. The pollen seasons were characterised by the occurrence of several-day-long interruptions in the presence of airborne pollen in the atmosphere of Lublin, which may indicate the long-distance transport of Ambrosia pollen.
A new stand, formed by some fifty individuals of Ambrosia artemisiifolia L., an invasive plant of alien origin, was discovered at the Katowice railway station (southern Poland), in 2011. Two years later, the stand (increased to more than 160 individuals in the meantime) was re-visited, phytosociological survey was made, and four soil cores (5 cm deep and 80 cm² surface area, each) were taken for seed bank studies. The phytosociological sampling revealed 28 co-occurring vascular species, most of them representing hemicryptophyte and geophyte life forms. According to Ellenberg's values, the species pool indicated sunny (L7 and L8 species dominating) and semi-dry (mainly F4 species) habitat for the common ragweed population, whereas Zarzycki's soil granulometric data reflected coarse-grained soil. Albedo of the soil was lower than that of the vegetation thus sparsely vegetated sites were considered as heat accumulating microhabitats that might support establishment of the thermophilic A. artemisiifolia. During soil seed bank analysis a high number of naturally opened fruits (dehiscent achenes) were found (718.75 per m²) most probably indicating frequent germination in previous years. Greenhouse germination tests proved successful germination of 125 individuals per m², which was considered enough to maintain the A. artemisiifolia stand at the station. The results call attention to a newly discovered, established population with increasing demography of A. artemisiifolia, a highly allergenic introduced weed of Poland.
Airborne ragweed pollen concentration in the north-eastern part of Croatia was investigated in relation to some meteorological factors. Data was obtained for three consecutive years (2001, 2002 and 2003) using volumetric method (Burkard trap). The correlation between the concentration of pollen grains in the atmosphere and maximum, minimum and mean daily temperature, daily temperature range, sunshine hours, relative humidity and precipitation from daily monitoring was studied. The critical ragweed pollen season appears from 33rd to 38th week, with its peak at the beginning of September each year. Statistically significant correlation between the ragweed pollen count and selected meteorological variables were found in some study years, but only a correlation with the mean air temperature and ragweed pollen presence in the air was significant over time.
Common ragweed (Ambrosia artemisiifolia L.) is an invasive weed of fi eld crops and human-disturbed habitats in Europe. As well as in its natural range (North America), common ragweed is a threat to human health due to its abundant allergenic pollen release. Most studies have been focused on airborne pollen monitoring, but to date, no data have been available on precise individual plant pollen and seed production related to plant traits growing in natural environment and on their corresponding source of variation. The aim of this study was to evaluate pollen and seed production of common ragweed plants in several populations in France. Seasonal pollen production per plant ranged from 100 million to 3 billion and seed production from 346 to 6,114, depending on plant size and habitat. Common ragweed plants developing in fi eld crops produced more pollens and seeds than those growing in other habitats. Pollen and seed production was closely related to plant volume and biomass, thus providing a means of estimating potential pollen and seed production in given target areas. Such biological data could be integrated into population management strategies or into airborne pollen modelling.
The aim of the study was to determine the onset, duration and termination of the ragweed pollen season; intradiurnal, daily and monthly pollen variation, and possible differences in the occurrence and concentration of ragweed pollen according to sampling sites between 2002 and 2003. The study was conducted at three sampling sites in central Croatia over two pollen seasons (2002 and 2003) using the volumetric method of sampling (Hirst type sampler). In 2003, the ragweed pollen season was by 43% longer and the percentage proportion of ragweed pollen by 3.4% greater in comparison with 2002. The total ragweed pollen count and number of days with ragweed pollen concentration greater than 30 pollen grains per m3 air showed a declining tendency from East to West in both seasons. The intradiurnal peak concentration occurred between 10.00-14.00. The air concentration of ragweed pollen decreased with temperature decline and precipitation. Results of the study provided useful information to individuals allergic to ragweed pollen thereby allowing them to adjust their outdoor activities to avoid contact with the allergen.
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