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Alexandrium minutum cysts were studied in sediment cores from its type locality, the Eastern Harbour of Alexandria, following the disappearance of the species from the plankton since 1994. Three cores were sampled in the summer of 1999 along the north-south axis of the harbour. The sediments were subjected to grain size analysis and their organic carbon content was determined. The sediments consisted of medium, coarse and very coarse sand. Grain size and organic carbon content were negatively and significantly correlated in core 1 but followed a parallel trend in cores 2 and 3. Seven dinoflagellate cysts, representing 6 genera were identified from the cores. Their relative abundance showed a remarkable difference. A. minutum cysts contributed a maximum of 17.4% to the total cysts. The distribution profile of A. minutum cysts in the cores reflects the bloom duration but not its productivity. The cyst distribution in the cores is the resultant of two opposite processes, the sedimentation rate and the continuous erosion of the bottom sediments, which is not related to sediment texture.
This cross-sectional study was carried to determine the prevalence of intestinal parasitic infections among mentally handicapped individuals in Alexandria, Egypt, in the period from December 2012 till November 2013. The study was conducted on 200 institutionalized and non-institutionalized mentally handicapped individuals. Fresh stool samples were subjected to different stains including; trichrome for detecting intestinal protozoa, modified acid fast stain for intestinal coccidia and quick hot gram chromotrope stain for Microsporidia. Also they were processed by Kato-Katz and formol ethyl acetate techniques for intestinal helminths. Additionally, blood samples were collected for measuring hemoglobin levels. Out of 200 mentally handicapped individuals, 87 (43.5%) were infected. The infection rates were 44.6% and 42.6% for non-institutionalized and institutionalized people, respectively. Regarding gender, 46.7% and 38.5% were reported for the males and females respectively. The most common parasites detected were: Cryptosporidium sp. (23.5%), microsporidia (15%), Giardia lamblia (8.5%), Dientamoeba fragilis (8%), Cyclospora cyatanensis (7.5%), Blastocystis hominis (6.5%), Entamoeba histolytica (5.5%) and Entamoeba coli (2.5%). Rates for Isospora belli and Enterobius vermicularis were estimated to be 1.5% for each, while lower rate was reported for Iodamoeba butschlii (1.0%). Prevalence of infections among mentally handicapped individuals are indications for several risk factors, including improper sanitary hygiene and illiteracy about personal hygiene. Therefore, frequent investigations, health care and medical intervention are needed.
This study is concerned with the temporal changes in the levels of zinc and cadmium in the sediments of Nozha Hydrodrome during the past 100 years. Seven sediment core samples, covering the study area, were collected from the bottom of the Hydrodrome. A five-step sequential extraction technique was applied to determine the solid phase concentrations of zinc and cadmium. Zinc gives an idea of the quantities of sewage effluents, while cadmium provides an indication of the amounts of agricultural discharges. The vertical distribution curves show that the average total concentrations of zinc in the sediments increased at a rate of 2.5 μg g−1 y−1 from 1900 to 1950 and at 1.5 μg g−1 y−1 from 1950 to 1990. Since 1990 the zinc concentration in Nozha Hydrodrome sediments has been decreasing at 1.5 μg g−1 y−1. The average total cadmium concentration exhibits a different vertical distribution pattern: it increased at a rate of 0.42 μg g−1 y−1 from 1900 to 1950, after which it became constant from 1950 to 1970. Since 1970 it has been increasing at 0.53 μg g−1 y−1. The ongoing increase in cadmium concentrations in the sediments is due to the increase in agricultural discharges into the Hydrodrome, especially as significant amounts of phosphate fertilizers are used to nourish the soil around the Hydrodrome. The rise in cadmium concentrations since 1900 has been accompanied by a similar increase in zinc concentrations with time resulting from the discharge of untreated sewage into the Hydrodrome. In 1990 a sewerage system and sewage treatment plant came into operation, as a result of which discharges of domestic effluent into the Hydrodrome ceased. Since then the amount of zinc in sediments has been decreasing steadily.
A marine Bacillus strain was isolated from the eastern harbour of Alexandria and identified as Bacillus sp. MIG. Maximum activity of studied proteases was obtained when the bacterium was grown in medium with 1 % wheat bran and 0.5% yeast extract in addition to the mineral salts and incubated for 48 h at 30°C and 120 rpm. Two alkaline proteases (Pro 1 and Pro 2) were purified to homogeneity using cation exchange chromatography on CM-Sepharose CL-6B followed by Sephadex G-75 superfine. The optimum activities were at pH 11 or 12, and temperatures of 50 and 55°C for Pro 1 and Pro 2 respectively. These two enzymes were relatively stable over pH range from 7.0-11. Pro 2 was found to be more stable at 50°C in absence of Ca²⁺ and retained about 47% of its activity after 3 h at this temperature, while Pro 1 lost its activity completely at the same conditions. The two enzymes were active against haemoglobin and casein; in addition, Pro 2 exhibited moderate activity against keratin. Both enzymes were partially inhibited by Ag⁺ and Hg²⁺. PMSF completely inhibited the enzymes, while dithiothreitol and 2-mercaptoethanol stimulated their activities, suggesting to be thiol-dependent serine proteases. The enzymes were stable in the presence of the surfactants and bleaching agent (H₂O₂) and relatively stable in presence of some commercial detergents.
Statistical analyses of data concerning the phytoplankton standing crop and biomass were applied as a tool for assessing pollution in El-Dekhaila Harbour. Univariate and multivariate analysis showed a succession of three clusters associated with salinity and temperature variations. The first and third clusters comprised mainly diatoms and dinoflagellates. The second cluster, richer in species, was dominated by brackish water cyanophytes, chlorophytes and euglenophytes. The diversity index ranged from 0.08 to 2.41. A high diversity was associated with high evenness, reflecting the multidominance pattern of cluster (2). On the other hand, multiple correlations between salinity and standing crop, richness and evenness, were negative and significant, but positive with the diversity index. The ABC curve showed that the k-dominance curve for numerical abundance extends above that for the biomass when clusters 1an d 3 are dominant. Both curves coincide more or less for another segment of the curve but when cluster 2 becomes dominant the numerical abundance curve runs below that of the biomass. Such a pattern may indicate unpolluted or moderate to heavily polluted conditions in El-Dekhaila Harbour. The present study reveals that the combination of univariate and multivariate analysis with the ABC curve provides a promising tool for the characterization of phytoplankton dynamics under stress conditions. On the other hand, the phytoplankton community in El-Dekhaila Harbour is stressed not by the inflow of brackish water but rather by the inflow of sea water. This is indicated by the higher diversity and evenness, and by the ABC curve during the dominance of cluster (2).
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