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A new identification method for Clarias gariepinus, Clarias macrocephalus and their hybrids using PCR-RFLP

100%

Panicz R., Sadowski J., Hofsoe-Oppermann P., Polgesek M.

Electronic Journal of Polish Agricultural Universities. Series Fisheries

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2018

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tom 21

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nr 4

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Mode of attachment and histopathological effects of Macrogyrodactylus clarii, a monogenean gill parasite of the catfish Clarias gariepinus, with a report on host response

100%

Arafa S. Z., El-Naggar M. M., El-Abbassy S. A.

Acta Parasitologica

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2009

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tom 54

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nr 2

103-112

A detailed account is given about the mode of attachment and histopathological effects of Macrogyrodactylus clarii Gussev, 1961, a viviparous monogenean from the gills of the catfish Clarias gariepinus. Most parasites attach their haptors to the proximal region of the gill filament (primary gill lamellae), while few specimens were seen attached to the distal region. Attachment of the haptor was achieved mainly by the blade of the hamuli, but no evidence was found indicating the participation of marginal hooklets in the attachment. The hamuli of M. clarii penetrate into the interlamellar epithelium of the gill tissue. Some evidences were found to indicate that M. clarii may also utilize suction force during haptoral attachment. The pathological effects of M. clarii are manifested by breakdown of the coating epithelium, necrosis of the epithelial cells, vacuolations inside and outside the host cells, fusion of the gill lamellae, rupture of blood capillaries, infiltration of erythrocytes and degeneration and fibrosis of the interlamellar epithelium. The host response includes the appearance of lymphocytes, mucoid secretions and hyperplasia of the tissue at the site of attachment.

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Acute and sub lethal effect of potassium cyanide on the behaviour and ATPase enzyme activity in the freshwater fish, Clarias gariepinus (Catfish)

80%

Oseni K.

International Letters of Natural Sciences

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2015

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tom 49

The Present research studies the acute sub lethal toxicity of potassium cyanide (KCN) in the fresh water, Clarias gariepinus and the effects on its behaviour and the Na+ - K+ ATPase enzyme activity. Acute toxicity of potassium cyanide (free cyanide) to the freshwater fish was studied using static bioassay method over a period of 96 h. Different concentrations of the toxicant (KCN) were used and LC50 value was found to be 361μg/L. Behavioural changes when exposed to lethal concentration of KCN showed increased feed intake, cannibalism, irregular swimming activity, rapid jerk movement, aggressiveness, loss of balance, opercula movement, surface behaviour, loss of equilibrium, change in body colour and convulsion. The effect of potassium on the Na+ - K+ ATPase of various physiological tissues which includes the gill, liver, muscles and intestinal over duration 0f 12 h- 35 days were also studied. Cyanide intoxication resulted in marked changes in ATPase, shown by significant decrease in the enzyme activities. The result shows that ATPase enzyme together could be employed as a sensitive and useful biomarkers for cyanide pollution while the behavioural changes could be an early signal of toxicity.

Ograniczanie wyników

1 Acta Parasitologica

1 Electronic Journal of Polish Agricultural Universities. Series Fisheries

1 International Letters of Natural Sciences

1 Arafa S. Z.

1 El-Abbassy S. A.

1 El-Naggar M. M.

1 Hofsoe-Oppermann P.

1 Oseni K.

1 Panicz R.

1 Polgesek M.

1 Sadowski J.

1 2018

1 2015

1 2009

A new identification method for Clarias gariepinus, Clarias macrocephalus and their hybrids using PCR-RFLP

Mode of attachment and histopathological effects of Macrogyrodactylus clarii, a monogenean gill parasite of the catfish Clarias gariepinus, with a report on host response

Acute and sub lethal effect of potassium cyanide on the behaviour and ATPase enzyme activity in the freshwater fish, Clarias gariepinus (Catfish)