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1

Ocena chorobotworczosci Aeromonas hydrophila izolowanych od ryb na terenie wojewodztwa olsztynskiego

100%

Andziak J., Anusz Z., Jakubczak A.

Medycyna Weterynaryjna

|

1992

|

tom 48

|

nr 02

54-56

The evaluation of potential pathogenicity of A. hydrophila strains isolated from healthy fish was carried out on the basis of their ability to produce enterotoxins, beta — haemolysins and to haemagglutinate blood cells in humans and guinea pigs. The studies comprised 29 strains of A. hydrophila isolated from 67 fish from 6 fish — breeding stations in the Olsztyn province. No correlation was found between the ability of the examined strains to agglutinate human and guinea pig erythrocytes, and the enterotoxicity and hemolitic activity. Some correlation was found between the enterotoxicity of the strains and their hemolitic activity. Incubation of A. hydrophila strains at temp. 25°C allows to detect more enterotoxic strains (31% strains) than at temp. 37°C (13% strains).

2

Survival of Escherichia coli and Aeromonas hydrophila in non-carbonated mineral water

88%

Korzeniewska E., Filipkowska Z., Zarnoch D., Tworus K.

Polish Journal of Microbiology

|

2005

|

tom 54

|

nr Suppl.

The study was carried out on the survival of Escherichia coli and Aeromonas hydrophila in samples of mineral waters. Enumeration of the bacteria was performed by spread inoculation of samples (0.1 cm³) over the surface of selected media in Petri plates. Twenty bottles (four bottles of each of the five brands) of non-carbonated mineral waters with different levels of dissolved solids and organic content were chosen to study every strain. Ten bottles were stored at 4°C, the other ten were kept at 22°C. Half of the samples of mineral water was filtered, the remaining water was unfiltered. The resulting growth curves depended on the time of storage. The number of E. coli increased during the first two weeks (except the seventh day) and decreased during the next days. E. coli was detected in 70% of samples of water after 182 days. The number of A. hydrophila decreased during the first three days, increased on the seventh day and decreased during the next days. A. hydrophila was detected in 15% of the samples of water after 182 days. The temperature of storage was inessential for growth. The most important factors were the brand and the filtering or unfiltering of water. The highest numbers of the bacteria analysed were detected in filtered water, irrespective of the water brand and temperature of storage.

3

Zakazenie Aeromonas hydrophila przyczyna padniec dzikich kaczek

75%

Zbikowski A., Szeleszczuk P., Karpinska E., Rzewuska M., Malicka E., Binek M.

Medycyna Weterynaryjna

|

2006

|

tom 62

|

nr 06

720-722

During the summer heat wave of July 2004, a large number of mallard ducks (Anas platyrhynchos) were found dead in Skaryszewski Park in Warsaw. Simultaneously, in the parks lake a massive wave of dead fish were discovered. Gram negative oxydase positive rods were isolated from the following organs of the dead ducks: kidneys, liver, pancreas and intestine, as well as in a pure culture. These bacteria caused a strong haemolyis on blood agar plates. Using API, NE test isolates were identified as Aeromonas hydrophila. The tested strain was proven susceptible, among others, to neomycine, streptomycine, gentamycine, tetracycline, flumequine, and norfloxacine, but resistant to amoxycillne, amoxycillin and clavulanic acid as well as to polimyxin.

4

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Preliminary investigations on the influence of some factors on the number of skin mucous cells in carp [Cyprinus carpio]

75%

Antychowicz J., Kozinska A., Kozaczynski W., Roszkowski J.

Bulletin of the Veterinary Institute in Puławy

|

1994

|

tom 38

|

nr 1

5

Immunisation of carp [Cyprinus carpio L.] against motile Aeromonas

75%

Kozinska A., Antychowicz J.

Bulletin of the Veterinary Institute in Puławy

|

2000

|

tom 44

|

nr 1

The purpose of the study was to evaluate an optimum dose of Aeromonas hydrophila and Aeromonas sobria antigens for the vacccination of carp (Cyprinus carpio L.) and to compare the effectiveness of the vaccination with the antigens inactivated with formalin or thermally and given intraperitoneally (ip) or by immersion (imm). The dose was evaluated with the use of formalin antigens. Doses ranging from 3 x 10⁴ to 6 x 10⁸ cells were given by injection and doses of 3 x 10⁵ to 6 x 10⁶ cells/mL of water were administered in bath. Experiments were conducted at 12°C, 16°C, and 23°C ± 1°C . To compare the effectiveness of the antigens inactivated with formalin and thermally the two doses 3 x 10⁸ cells (ip) and 5- 6 x 10⁷cells/mL (imm) were used. The effect of immunisation was evaluated with challenge tests. The relative percentage of health (RPH) and relative percentage of survival (RPS) of fish were calculated. Doses of 3 x 10⁸ cells, and 5-6 x 10⁷ cells/mL given ip and imm, respectively, were considered the most effective irrespective of the water temperature. No marked differences were found between the administration of the antigen by injection and immersion. Fish manifested a significantly higher level of immunity after the administration of formalin antigen in comparison to that following the administration of the antigen inactivated thermally.

6

Proba zastosowania metody immunohistochemicznej do wykrywania antygenu bakteryjnego u ryb

71%

Sopinska A., Szubstarska D.

Acta Scientiarum Polonorum. Medicina Veterinaria

|

2002

|

tom 01

|

nr 2

67-75

7

Use of herbal concoction in the therapy of goldfish [Carassius auratus] infected with Aeromonas hydrophila

63%

Harikrishnan R., Balasundaram C., Moon Y.-G., Kim M.-C., Kim J.-S., Heo M.-S.

Bulletin of the Veterinary Institute in Puławy

|

2009

|

tom 53

|

nr 1

27-36

The aim of the presented study was testing the efficacy of an herbal concoction in goldfish (Carassius auratus) experimentally infected with Aeromonas hydrophila. After an intramuscular injection of the pathogen, the scales sloughed off on the site of the injection, with an appearance of a muscular haemorrhagic protuberance, which progressed into an extensive ulcerative dermatitis associated with focal haemorrhage, oedema, and dermal necrosis exposing the underlying muscle. The progression of the disease affected organs in the following order: muscle, gills, liver, and finally the heart. The dip treatment with the 1% herbal concoction for 5 min daily restored the macro- and microscopical structure of the altered primary gill lamellae, liver, heart, and muscles. The recovery changes were described.

8

Wplyw promieniowania jonizujacego na przezywalnosc Aeromonas hydrophila w przetworach mlecznych

63%

Szczawinski J., Stanczak B., Peconek J.

Medycyna Weterynaryjna

|

2002

|

tom 58

|

nr 05

368-370

9

Aktywnosc hemolityczna i proteolityczna jako wskazniki patogennosci bakterii Aeromonas hydrophila i A.sobria dla karpi [Cyprinus carpio]

63%

Rogulska A., Antychowicz J., Zelazny J.

Medycyna Weterynaryjna

|

1994

|

tom 50

|

nr 02

55-58

The pathogenicity of 68 strains of A. hydrophila and 44 of A. sobria was assessed in two-year-old carp (Cyprinus carpio L.). The fish were selected from erythro- dermatitis-free farms and were adapted to the laboratory environment for one month; then they were infected with a subepidermal bacterial suspension. The inoculum contained 107 bacterial cells in 0,2 ml of 0.85 per cent beffered salt solution (PBS). The haemolytic activity was tested on horse blood agar (a base with 5 per cent defibrinated blood) and the proteolytic activity on a base with 15 per cent of gelatine. The individual strains of A. hydrophila and A. sobria displayed different haemolytic and proteolytic activity and also different pathogenicity. Pronounced haemolytic activity was found in 93 per cent of highly pathogenic strains of A. hydrophila and in 87.5 per cent of distinctly pathogenic strains of A. sobria. The above properties were also noted in the 45 per cent and 10 per cent mildly pathogenic strains, respectively. However, these characteristics were also found in 15 per cent nonpathogenic strains of A. hydrophila. Combined pronounced haemolytic and proteolytic activity were displayed in 90 per cent of highly pathogenic strains of A. hydrophila and 87.5 per cent of A. sobria. Only one non-pathogenic strain of A. hydrophila also possessed a marked haemolytic and proteolytic activity.

10

Wpływ immunostymulacji karpi LPS Aeromonas hydrophila na odporność nieswoistą i wrażliwość na zakażenie w teście challenge

63%

Grochola A., Sopinska A., Puk K.

Medycyna Weterynaryjna

|

2015

|

tom 71

|

nr 03

Bacterial infections are a serious problem for commercial farms of carp (Cyprinus carpio L.) in Poland. Given the absence of efficient vaccines, it is crucial to search for new agents enhancing the non-specific immune response of the fish. In the present study, immunomodulating effects of lipopolysaccharide extracted from the virulent (LPS-P) and non-virulent (LPS-NP) strains of Aeromonas hydrophila were studied. For that purpose, different concentrations of LPS-P and LPS-NP (25, 50, and 75 µg/100 g body weight) were administered to test animals through intraperitoneal injection. The non-specific immune response of the fish were studied at days 7, 14, 21, 28, and 60 after vaccination. Immunostimulated fish showed an increase in total leukocyte count, in the population of monocytes and neutrophils, and in immature cell count in the whole blood. Phagocytic activity and the production of reactive oxygen species were also elevated after vaccination. Moreover, vaccinated fish showed a significant increase in serum lysozyme. The results of the present study show that LPS-NP has a greater immunostimulatory effect than LPS-P at doses of 50 and 75 µg/100 g body weight. In addition, 7 days after vaccination with LPS-NP (50 µg/100 g body weight), fish were challenged with the virulent strain of A. hydrophila. The relative percentage of survival in groups 1 and 2 was 58.82% and 76. 47%, respectively, which indicates that the administration of LPS-NP makes C. carpio more resistant to infection by A. hydrophila

11

Zakazenia Aeromonas hydrophila u drobiu, ptakow ozdobnych,ptakow z ogrodow zoologicznych i wolnozyjacych

63%

Glunder G.

Zeszyty Naukowe Akademii Rolniczej we Wrocławiu. Weterynaria

|

1992

|

tom 52

77-83

12

Wplyw wysokiego cisnienia hydrostatycznego na przezywalnosc Aeromonas hydrophila w serze podpuszczkowym dojrzewajacym

63%

Szczawinski J., Stanczak B., Peconek J.

Medycyna Weterynaryjna

|

2004

|

tom 60

|

nr 03

314-316

13

Izolacja szczepow Aeromonas hydrophila ze slimakow jadalnych

63%

Staroniewicz Z., Krol J.

Życie Weterynaryjne

|

1996

|

tom 71

|

nr 01

16-17

14

Występowanie niektórych gatunków pałeczek gram-ujemnych z rodzajów Yersinia, Klebsiella, Aeromonas oraz ich rola w etiologii zatruć pokarmowych

63%

Maciejska-Roczan K.

Roczniki Państwowego Zakładu Higieny

|

1979

|

tom 30

|

nr 3

15

Influence of an experimental Aeromonas hydrophila vaccine on selected haematological values and non-specific immunity in carp [Cyprinus carpio L.]

63%

Kozinska A., Antychowicz J.

Bulletin of the Veterinary Institute in Puławy

|

2000

|

tom 44

|

nr 2

The studies aimed to evaluate the effect of experimental Aeromonas hydrophila vaccine on the total number of leukocytes, the number of neutrophils and T-lymphocytes and the phagocytic activity of the neutrophils and monocytes of carp (Cyprinus carpio L). The vaccine was given intraperitoneally or in an 1-hour bath. The number of leukocytes and the phagocytic activity were examined several times during a 42-day period after immunisation. Examinations were conducted at 12°C and 23°C. A visible increase of the total number of leukocytes and T-lymphocytes (21 days after immunisation) and neutrophils (7 to 14 days after immunisation) was found at 12°C. The percentage of engulfing phagocytes and their activity were significantly higher than those in the controls during the whole experimental period. A marked decrease in the total number of leukocytes was seen as early as 14 days after immunisation at 23°C; moreover, the number of neutrophils and T-lymphocytes was significantly lower on day 21 post immunisation as compared to that in the controls. On the other hand, the phagocytic activity was visibly higher than that in control fish but differences were less marked than those at 12°C.

16

High pressure processing for food safety

63%

Fonberg-Broczek M., Windyga B., Szczawinski J., Szczawinska M., Pietrzak D., Prestamo G.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 3

Food preservation using high pressure is a promising technique in food industry as it offers numerous opportunities for developing new foods with extended shelf-life, high nutritional value and excellent organoleptic characteristics. High pressure is an alternative to thermal processing. The resistance of microorganisms to pressure varies considerably depending on the pressure range applied, temperature and treatment duration, and type of microorganism. Generally, Gram-positive bacteria are more resistant to pressure than Gram-negative bacteria, moulds and yeasts; the most resistant are bacterial spores. The nature of the food is also important, as it may contain substances which protect the microorganism from high pressure. This article presents results of our studies involving the effect of high pressure on survival of some pathogenic bacteria - Listeria monocytogenes, Aeromonas hydrophila and Enterococcus hirae - in artificially contaminated cooked ham, ripening hard cheese and fruit juices. The results indicate that in samples of investigated foods the number of these microorganisms decreased proportionally to the pressure used and the duration of treatment, and the effect of these two factors was statistically significant (level of probability, P ≤ 0.001). Enterococcus hirae is much more resistant to high pressure treatment than L. monocytogenes and A. hydrophila. Mathematical methods were applied, for accurate prediction of the effects of high pressure on microorganisms. The usefulness of high pressure treatment for inactivation of microorganisms and shelf-life extention of meat products was also evaluated. The results obtained show that high pressure treatment extends the shelf-life of cooked pork ham and raw smoked pork loin up to 8 weeks, ensuring good micro-biological and sensory quality of the products.

17

Numerical analysis of electrophoretic periplasmic protein patterns of Aeromonas sp. strains

63%

Szczuka E., Kaznowski A.

Polish Journal of Microbiology

|

2005

|

tom 54

|

nr 2

A total of 103 strains of Aeromonas spp. isolated from clinical and from environmental samples was compared by using SDS-PAGE of periplasms proteins patterns. Strains isolated from Polish children suffering from gastroenteritis did not appear similar to strains isolated from human living in Hong-Kong. Aeromonas sp. strains did not show a tendency to cluster according to their origin. Our results have demonstrated no species-specifs periplasms protein profiles. A significant protein electrophoretic heterogeneity was observed within the species A. hydrophila, A. hestiarum, A. salmonicida, A. caviae, A. media, and A. vermin biotype sobria.

18

Occurrence and activity of the chitinolytic bacteria of Aeromonas genus

63%

Swiontek B. M., Donderski W.

Polish Journal of Environmental Studies

|

2001

|

tom 10

|

nr 1

A study was carried out on the occurrence and activity of chitinolytic planktonic bacteria of Aeromonas sp., Aeromonas hydrophila and Aeromonas salmonicida species, isolated from lake Jeziorak. Among the identified strains decomposing chitin the most abundant were Aeromonas sp.. All the investigated strains showed maximum chitinolytic activity at pH 6.0. An increase in chitinolytic activity was observed that occurred along with temperature growth (10° - 40°C) and colloidal chitin concentration in the medium (0.5 - 2.5%). Their decomposing activity was most intense after a 192 h incubation time. No strain displayed activity after a 48 h incubation time.

19

Influence of thrombocyte activity on the susceptibility of the carp [Cyprinus carpio L.] to Aeromonas hydrophila infection at different temperatures

63%

Kozinska A., Antychowicz J., Kostrzewa P.

Bulletin of the Veterinary Institute in Puławy

|

1999

|

tom 43

|

nr 1

Three groups of carps were kept for 6 to 12 weeks at 11°C, 16°C and 23°C. A portion of the fish from each group was infected with Aeromonas hydrophila. Experimental fish were subjected to evaluation of number of thrombocytes, the index of bacteria-thrombocyte adherence (IB-TA), the percentage of active thrombocytes (%AT) and the index of thrombocyte aggregation (ITA). Carps kept at 11℃ revealed the highest values of the indices examined whereas carps kept at 23℃ the lowest ones. The indices were examined singly bcfore the bacteria administration. Fish demonstrating the lowest values of the indices were most sensitive to the infection and revealed the highest mortality rate. In contrast, fish with the highest values of the indices revealed the highest immunity. These relationships were obvious mainly at 11°C whereas at 23°C, by contrast, they were less distinct.

20

Antibiotic susceptibility of Aeromonas hydrophila and A. sobria isolated from farmed carp [Cyprinus carpio L.]

63%

Guz L., Kozinska A.

Bulletin of the Veterinary Institute in Puławy

|

2004

|

tom 48

|

nr 4

Ograniczanie wyników

Ocena chorobotworczosci Aeromonas hydrophila izolowanych od ryb na terenie wojewodztwa olsztynskiego

Survival of Escherichia coli and Aeromonas hydrophila in non-carbonated mineral water

Zakazenie Aeromonas hydrophila przyczyna padniec dzikich kaczek

Preliminary investigations on the influence of some factors on the number of skin mucous cells in carp [Cyprinus carpio]

Immunisation of carp [Cyprinus carpio L.] against motile Aeromonas

Proba zastosowania metody immunohistochemicznej do wykrywania antygenu bakteryjnego u ryb

Use of herbal concoction in the therapy of goldfish [Carassius auratus] infected with Aeromonas hydrophila

Wplyw promieniowania jonizujacego na przezywalnosc Aeromonas hydrophila w przetworach mlecznych

Aktywnosc hemolityczna i proteolityczna jako wskazniki patogennosci bakterii Aeromonas hydrophila i A.sobria dla karpi [Cyprinus carpio]

Wpływ immunostymulacji karpi LPS Aeromonas hydrophila na odporność nieswoistą i wrażliwość na zakażenie w teście challenge

Zakazenia Aeromonas hydrophila u drobiu, ptakow ozdobnych,ptakow z ogrodow zoologicznych i wolnozyjacych

Wplyw wysokiego cisnienia hydrostatycznego na przezywalnosc Aeromonas hydrophila w serze podpuszczkowym dojrzewajacym

Izolacja szczepow Aeromonas hydrophila ze slimakow jadalnych

Występowanie niektórych gatunków pałeczek gram-ujemnych z rodzajów Yersinia, Klebsiella, Aeromonas oraz ich rola w etiologii zatruć pokarmowych

Influence of an experimental Aeromonas hydrophila vaccine on selected haematological values and non-specific immunity in carp [Cyprinus carpio L.]

High pressure processing for food safety

Numerical analysis of electrophoretic periplasmic protein patterns of Aeromonas sp. strains

Occurrence and activity of the chitinolytic bacteria of Aeromonas genus

Influence of thrombocyte activity on the susceptibility of the carp [Cyprinus carpio L.] to Aeromonas hydrophila infection at different temperatures

Antibiotic susceptibility of Aeromonas hydrophila and A. sobria isolated from farmed carp [Cyprinus carpio L.]