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Effect of different drying conditions for the viability of Candida albicans present on carriers

100%

Rohm-Rodowald E., Chojecka A., Jakimiak B., Wiercinska O., Ziemba B., Kanclerski K.

Roczniki Państwowego Zakładu Higieny

2014

tom 65

nr 1

Background. Problems in substantial under recovery of Pseudomonas aeruginosa and Candida albicans from carriers have been demonstrated for laboratories performing phase 2, step 2 efficacy tests on disinfectants relative to levels required by the EN 13697 standard. It was thus necessary to determine recoveries of these microorganisms following procedural losses incurred during drying and to optimise drying conditions such that recoveries then complied with the standard. Objectives. The aim of the study was to establish optimal drying conditions for the recovery of Candida albicans ATCC 10231 from carriers. Materials and Methods. The evaluation was performed according to the EN 13697:2001 standard procedure. A test suspension of Candida albicans and interfering substance were inoculated onto the surface of carriers (2 cm diameter stainless steel discs) and then dried under different conditions consisting of: a 37°C incubation with and without an incubator fan as well as at 23°C (room temperature) in a laminar air flow cabinet. Carriers were dried until the surfaces appeared visibly dry and the number of surviving organisms then recovered from the surface were quantified. The following were calculated for colony forming units (cfu); N (log10 cfu in a 0.05 ml test suspension), NC (the control log10 cfu in neutralizing medium), Nts (cfu numbers remaining on the surface) and the N-NC difference which should not exceed 2 log10 when microorganism recoveries are adequate and without any toxicity effects of the neutralising medium. Experiments was conducted using validating procedure (NC) which is performed with distilled water. Results. Drying at 37°C adversely affected the survival of Candida albicans and prevented the levels of microbial recovery from carriers to reach those specified by the EN 13697 standard. However, drying at around room temperatures of 23°C reduced Candida albicans mortality and increased recoveries from the carrier to levels compliant with the standard, where the N-NC differences were not greater than 2 log10. Conclusions. The viability of Candida albicans ATCC 10231 is sufficiently improved when carriers are dried at 23°C, even if the drying time exceeds 60 minutes. The density of the initial test suspension (N) should also be increased.

Susceptibility of Acanthamoeba to multipurpose lens-cleaning solutions

67%

Aguiar A. P. C., Oliveira Silveira C., Todero Winck M. A., Rott M. B.

Acta Parasitologica

2013

tom 58

nr 3

The present study investigated the susceptibility of Acanthamoeba spp. trophozoites to two multipurpose systems for cleaning and maintenance of contact lenses. Three strains of trophozoites from the ATCC (A. castellani T4, A. castellani Neff, and A. polyphaga) and two Acanthamoeba isolates obtained from swimming pools (PT5 and PO1) were placed in monoxenic culture. To test their survival in cleaning solutions for contact lenses, the trophozoites were exposed for 4 and 24 h to two multipurpose solutions (A and B), and were then inoculated into a new monoxenic culture. Amoebic growth on the plates was observed after 72 h of incubation. Trophozoites from all three ATCC strains and one isolate from a swimming pool (PO1) grew in all plates after 4 h of exposure to solutions A and B. After 24 h, the ATCC strains and the PO1 isolate showed growth in most of the plates treated. Only the PT5 isolate showed susceptibility to both solutions over the time intervals tested. The two solutions were not completely effective against most strains and isolates over the time intervals tested. These results are important, since species of Acanthamoeba are widely distributed in the environment and are potential agents of eye pathologies.

Ograniczanie wyników

1 Acta Parasitologica

1 Roczniki Państwowego Zakładu Higieny

1 Aguiar A. P. C.

1 Chojecka A.

1 Jakimiak B.

1 Kanclerski K.

1 Oliveira Silveira C.

1 Rohm-Rodowald E.

1 Rott M. B.

1 Todero Winck M. A.

1 Wiercinska O.

1 Ziemba B.

1 2014

1 2013

Wyniki wyszukiwania

Effect of different drying conditions for the viability of Candida albicans present on carriers

Susceptibility of Acanthamoeba to multipurpose lens-cleaning solutions