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1

Microbiological conversion of pure and crude glycerol to 1,3-propanediol

100%
Orczyk D., Szymanowska-Powalowska D., Leja K.
Acta Scientiarum Polonorum. Biotechnologia
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2012
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tom 11
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nr 3
1,3-propanediol (1,3-PD) is the important product used in chemical industry. Microbiological synthesis of 1,3-PD from crude glycerol is a good solution, both from an economic and environmental point of view. The aim of this work was to investigate the ef­fect of raw material (pure and crude glycerol) on the efficiency of the synthesis of 1,3-PD by the bacteria Clostridium butyricum DSP1 and Clostridium butyricum DO14 isolated from the samples taken from natural environment. Two strains of C. butyricum were simul­taneously investigated. The obtained results showed that the concentration of 1,3-PD was slightly lower in the case of crude glycerol than in pure glycerol, for both strains. Moreover, waste glycerol was not completely utilized.
2

Hypothetical glycerol pathways of newly isolated strains capable of 1,3-propanediol production

100%
Leja K., Samul D., Drozdzynska A., Myszka K., Juzwa W., Pawlicka J., Czaczyk K.
Acta Biochimica Polonica
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2014
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tom 61
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nr 4
3

1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum 2CR371.5

84%
Dabrowski S., Pietrewicz-Kubicz E., Zablotna E., Dlugolecka A.
Acta Biochimica Polonica
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2012
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tom 59
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nr 3
 1,3-propanediol is used as a monomer in the production of some polymers e.g. polytrimethylene terephthalate used in the production of carpets and textile fibers and in the thermoplastics engineering. However, the traditional chemical synthesis is expensive, generates some toxic intermediates and requires a reduction step under high hydrogen pressure. Biological production of 1,3-propanediol could be an attractive alternative to the traditional chemical methods. Moreover, crude glycerol which is a by-product of biodiesel production, can be used. We constructed a recombinant Escherichia coli strain producing 1,3-propanediol from glycerol by introducing genes of the dha operon from Clostridium butyricum 2CR371.5, a strain from our collection of environmental samples and strains. The E. coli strain produced 3.7 g of 1,3-propanediol per one litre of culture with the yield of 0.3 g per 1 g of glycerol consumed.
4

Isolation and identification of Clostridium spp. from natural samples that performs effective conversion of glycerol to 1,3-propanediol

84%
Leja K., Myszka K., Kubiak P., Wojciechowska J., Olejnik-Schmidt A. K., Czaczyk K., Grajek W.
Acta Scientiarum Polonorum. Biotechnologia
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2011
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tom 10
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nr 2
Indigenous bacteria in the natural environment can product a wide range of metabolites more efficiently. The aim of this work was to isolate from the natural environment non-pathogenic Clostridium strains that are able to convert glycerol to 1,3-propanediol and other metabolites of potential uses in industry. The effective methods of selection and maintenance of anaerobic cultures in the laboratory conditions were also investigated. Samples were pre-cultured on modified PY medium consisted 50 g/l of glycerol. Isolated colonies growth on TSC medium were screened on the basis of morphological characters typical for Clostridium sp. Isolated bacterial strains were allowed to growth in selective media such as RCM and modified PY. The metabolites of bacteria were investigated by the HPLC technique. The bacteria strains were identified by 16S rRNA technique. The highest percentage of isolates of the genus Clostridium were obtained from excrements of animals, compost, and silages. Nearly 60% were able to convert glycerol to 1,3-propanediol. The highest capacity for utilization of glycerol to 1,3-propanediol was observed in case of the species of Clostridium bifermentans and Clostridium sordelli. The most of examined microflora were also able to short-chain organic acids and ethanol synthesis.
5

Screening of environmental samples for bacteria producing 1,3-propanediol from glycerol

84%
Dabrowski S., Zablotna E., Pietrewicz-Kubicz D., Dlugolecka A.
Acta Biochimica Polonica
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2012
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tom 59
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nr 3
 Twenty nine environmental samples were screened for the presence of anaerobic microorganisms fermenting glycerol with 1,3-propanediol as a final product. Seven samples were then selected for the next step of our research and eight bacteria strains were cultured anaerobically. Seven of them produced 1,3-propanediol with a yield of 0.47-0.58. Six of the the isolated microorganisms were then classified as Clostridium butyricum (four strains), C. lituseburense (one strain), and C. sartagoforme (one strain). We suggest that of all these strains C. butyricum 2CR371.5 is the best 1,3-propanediol producer as producing no lactate as a by-product and growing well on a glycerol-containing medium.
6
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Biotechnological production of 1,3-propanediol from crude glycerol

67%
Drozdzynska A., Leja K., Czaczyk K.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2011
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tom 92
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nr 1
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