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We studied an old growth deciduous forest seed bank to examine how its potential role in regeneration is shaped by natural forest environment. Our research questions were: is the spatial pattern of seed bank influenced by local variation in elevation, soil moisture and light intensity, and what is the impact of herb layer characteristics on seed bank pattern. We recorded species composition of the herb layer and seed bank on a 2 × 40 m study plot divided into 20 quadrates, situated in a natural oak-hornbeam forest, in the Białowieża Primeval Forest, (NE Poland). Soil cores were sampled from two soil layers (0–5 cm and 5–10 cm) yielding altogether 40 samples of a total 15.9 dm3 and 0.159 m². Seeds were extracted from soil samples under a microscope. Ellenberg indicator values were used to characterize light (L) and moisture (F) conditions. Relative quadrate elevation was averaged for nine points. There were 6.65 × 10³ seeds m⁻² in upper soil layer and 3.00 × 10³ seeds m⁻² in lower soil layer. Seed bank structure constituted of patches 6 m diameter in the upper soil layer and 4 m in the lower soil layer. Aggregated pattern of the seed bank was influenced by clumped distribution of plants in the herb layer. Seed bank species richness in the upper soil layer was correlated with moisture (r = 0.485, P =0.03) and light (r = 0.526, P = 0.0172) values. Seed densities were correlated with moisture (r = 0.848 P <0.0001 upper and r = 0.491 P = 0.0278 lower soil layer) and light (r = 0.803 P <0.0001 upper and r = 0.751 P = 0.0001 lower soil layer). Seed density in upper soil layer was negatively correlated with elevation (r =–0.485 P = 0.0422). Higher seed density and species richness of the seed bank associated with better light conditions and higher moisture is probably caused by higher seed production in favourable conditions, and factors promoting seed persistence in soil. Our results indicate, that even subtle changes in light, moisture and mean relative elevation can shape seed bank spatial pattern on a fine local scale, differentiating the response of this community to small scale disturbances present in natural forests.
Forest seed banks mostly studied in managed forests proved to be small, species poor and not reflecting aboveground species composition. Yet studies conducted in undisturbed communities indicate a different seed bank characteristic. Therefore we aimed at describing soil seed bank in an undisturbed forest in a remnant of European lowland temperate forests, the Białowieża Forest. We compared similarity between the herb layer and seed bank, similarity of seed bank between different patches, and dominance structure of species in the herb layer and in the seed bank of two related oak-hornbeam communities. We report relatively high values of Sorensen species similarity index between herb layer and seed bank of both patches. This suggests higher species similarity of the herb layer and soil seed bank in natural, unmanaged forests represented by both plots than in fragmented communities influenced by man. Although there was a set of core seed bank species present at both plots, yielding high Sorensen species similarity index values, considerable differences between plots in seed bank size and dominance structure of species were found, indicating spatial variability of studied seed bank generated by edaphic conditions. Dominance structure of species in the herb layer was not reflected in the underlying seed bank. This stresses, that natural forest regeneration cannot rely only on the seed bank, although some forest species are capable of forming soil seed banks. While forest seed banks may not reflect vegetation composition of past successional stages, they may inform on history and land use of a specific plot.
Studies on the soil seed banks of fallow lands of different ages were carried out on poor soil abandoned fields and in a fresh coniferous forest in north-eastern Poland. The size and diversity of seed banks was studied with the seedling emergence method. Species abundance (i), density (ii), number of species from different biological groups (iii) and distribution and mean LI value (iv) were analysed as the function of fallow land age. It was found that: (i) species diversity, number of species and ln of density are linear declining function of the fallow land age; (ii) for approx. 25 years the share of diaspores of identified species groups has been relatively similar. Seed banks of 40-50-year-old fallow lands are dominated by Calluna vulgaris, while the seed bank of the old fresh coniferous forest is dominated by dicotyledonous perennials and grasses; (iii) within the first 50 years of succession the persistence of seed banks measured by the Longevity Index increases gradually.
Thermophilous oak wood is the most species-rich forest habitat in the zone of mixed deciduous forests. A very limited amount of it has been saved in good condition. We investigated the principal hypothesis that the vegetation and seed banks, especially of the ancient forest species, are good indicators of habitat naturalness and its aptitude for restoration. Vegetation and seed bank sampling were carried out in fragments of forest with a known management and disturbance history over the past 80 years. We predicted that natural and anthropogenic transformations of tree stands would be significant factors shaping species composition and similarity of vegetation and seed banks. The closest similarity was observed between the seed banks of plots which were never logged. The least related to others was the seed bank of the logged site, whose soil was ploughed prior to tree replantation. The highest number of ancient forest species was recorded in the vegetation (33 species) and in the soil seed bank (21 species) of the least transformed patch of thermophilous oak forest. It was decreasing gradually with increase of the tree canopy cover on the research plots. Our results indicate also that the higher the coefficient of similarity between seed bank and vegetation, especially of the ancient forest species, the higher the forest’s naturalness. We conclude that restoration of thermophilous oak wood has the highest chances for success in patches with well preserved seed banks and vegetation.
The research was conducted on four patches of thermophilous oak wood in Białowieża Primeval Forest: A – with a woodstand: oak + approx. 30-year-old hornbeam + hornbeam brushwood; B – with a hornbeam stand formed by natural seed fall after logging (ca. 1920) oaks; C – after logging oaks and replanted (ca. 1965) with pine and oak; D – with a natural low-density oak stand. Species composition and seed bank density were estimated using the seedling emergence method. Seedling emergence was observed over two vegetation seasons. Research demonstrated that: 1) the species abundance of the seed banks depends on canopy cover (A, B approx. 50 species; C, D approx. 70 species); 2) the floristical similarity (Sørensen’s index) of the seed bank and ground vegetation is higher in the undisturbed patch D (0.50) than in disturbed patches (0.30-0.35); 3) species diversity in plots A, B, C, D (H’=12.5; 13.4; 15.5; 16.9) and seed bank density per m2 (432.5; 958.0; 1486.5; 2268.0) are negatively correlated with the degree of patch shading; 4) the average weight of diaspores in the seed banks of shady plots is lower (A, B approx. 0.003 g) than that of sunny plots (C, D approx. 0.08 g); 5) the share of long-lived diaspores increases in patches after logging.
One of the first steps to successful invasion of plant species that reproduce sexually is seed germination, which may be highly influenced by climatic conditions. We studied Poa annua, a cosmopolitan species found across all climatic zones and the only alien species that has successfully colonized the Antarctic. Our research questions were: (i) if harsh polar conditions restrict seed germination of P. annua and (ii) if the germination capacity of the Antarctic population of the species is due to high germination aptitude in the source population. We compared germination of seeds collected from eight populations around the world (maritime Antarctica, S Chile, W Argentina and E Argentina, NE USA, SW Croatia, C Poland and S Poland). We followed germination of seeds collected in the field and acquired from plants cultivated under unified optimal conditions. We found significant differences between populations in germination characteristics of seeds collected in the field. These could be associated with seed ripening in different locations. Seeds obtained under favorable conditions differed in stratification requirements. The germination potential of the Antarctic population is lowered by unfavorable polar conditions impacting seed maturation. Thus, the species’ invasion in the Antarctic seems highly restricted by the harsh environment. Environmental unsuitability may restrict invasions of other species in the same way potentially. However, this environmental barrier protecting Antarctica from invasions may be broken under a climate warming scenario.
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