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Cross-recognition of Fagaceae pollen allergen by IgE raised against allergen of Cupressus arizonica pollen

100%

Mazzitelli A., Caiola M. G.

Acta Biologica Cracoviensia. Series Botanica

2003

tom 45

nr 1

This study investigated cross-reactivity between allergens of Cupressaceae and Fagaceae pollen. Human IgE raised against Cupressus arizonica pollen allergen was used to demonstrate the presence of related allergens on ultrathin sections of Fagaceae pollen (Quercus ilex, Castanea sativa, Fagus sylvatica). Tissue localization of the cross-reactive allergen was investigated by immunogold electron microscopy. TEM observations showed that IgE raised against C. arizonica allergen recognizes epitopes on Fagaceae pollen. The cross-reactive allergens can be found on the wall and in the cytoplasm.

Cytoplasmic reserves in generative and vegetative cells of Hermodactylus tuberosus Mill.: Cytochemistry, ESI and EELS analyses

100%

Caiola M. G., Canini A.

Acta Biologica Cracoviensia. Series Botanica

2003

tom 45

nr 1

This study uses cytochemical tests, electron spectroscopic imaging and electron energy loss spectroscopy techniques to identify and localize the reserves inside the generative cell of Hermodactylus tuberosus pollen. Cytochemical probes applied to sections observed by light and transmission electron microscopy indicated that the generative cell contains large osmiophilic bodies probably made of phytic acid rich in P and Ca. The significance of the rich granulations in generative cells of Hermodactylus pollen is discussed in relation to floral biology and environmental conditions. In comparison, the vegetative cytoplasm contains (a) lipid droplets formed by unsaturated lipids and related to vacuoles, (b) lipid bodies with larger dimensions, irregular in shape and very rich in Ca, (c) bodies stained in polysaccharide tests as well as lipid probes tentatively identified as glycolipid granulations, and (d) small granules very rich in P and Ca interpreted as phytin granules.

Ograniczanie wyników

2 Acta Biologica Cracoviensia. Series Botanica

2 Caiola M. G.

1 Canini A.

1 Mazzitelli A.

2 2003

Wyniki wyszukiwania

Cross-recognition of Fagaceae pollen allergen by IgE raised against allergen of Cupressus arizonica pollen

Cytoplasmic reserves in generative and vegetative cells of Hermodactylus tuberosus Mill.: Cytochemistry, ESI and EELS analyses