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Chlorophyll fluorescence, especially the optimum quantum yield Fv/Fm, is used to quantify the physiological state of plants, because stress, infections or wilting may lower the optimum quantum yield. The characteristics of chlorophyll fluorescence have attracted our attention for its use to determine the quality of spinach, because the measurement of Fv/ Fm during storage can be performed quickly and non-destructively, delivering information about spinach quality and its sale suitability. Applicability of modern techniques for quick and non-invasive in vivo determination of such parameters as quality and freshness of spinach under simulated sale conditions was tested in our study. In this experiment, application of chlorophyll fluorescence for quantification of quality as well as physical and physiological changes which occur in fresh products was verified. The experiment was conducted on spinach, a product characterized by rapid appearance of postharvest losses. Spinach was harvested as leafy vegetable for direct consumption and stored for 5 days (16 h in a cooling room at 2-4°C, 96-98% relative humidity, followed by 8 h at room temperature: ca 22oC, 65% relative humidity). Variants were represented by different cultivation conditions (fertilizer, leaf application of Previcur, CaCl2 and Cerone). The investigations revealed that fresh mass losses in the postharvest phase could be described by changes of Fv/Fm spinach leaves adapted to darkness by a linear relationship of parameters (r=0.77). The disadvantage of chlorophyll fluorescence measurement was the less precise determination of the critical loss of fresh mass of 3%. Instead, losses of 4 to 5% resulted in a the decrease of optimum quantum yield below the critical value of 0.80, which indicates the occurrence of postharvest stress. Leaf application of pesticides negatively influenced Fv/Fm. This relationship may be used to detect the application of plant protection products shortly before harvest, before the grace period is over. Hence, the determination of freshness using chlorophyll fluorescence offers, to a limited extent, protection of consumers. Further investigations showed that optimum quantum yield was linearly and positively correlated with the calcium content in spinach leaves. This relationship may indicate worse cell membrane integrity and reduced cell wall stability postharvest. Additionally, the decrease of Fv/Fm during storage may indicate the occurrence of cold stress as well as altering processes (senescence) and loss of quality of spinach, independent of its cultivation method. In these investigations, the relationship between chlorophyll fluorescence and color changes of leaves, content of chlorophyll, total nitrogen and water soluble carbohydrates, as well as respiration intensity and ethylene emission could not be found.
The present study of three vegetation periods evaluated the enzymatic browning process of two different commodities, carrot roots and apples. The influence of the genetic potential (effect of genus and cultivar), of magnesium fertilization is case of carrot and of storage (in case of carrot 6 months in a traditional natural mound and in case of apple 6 months under ultra low oxygen (ULO) conditions) were investigated. In the case of carrot 5 different cultivars were investigated, middle late 'Berjo', late: 'Flacoro', 'Karotan', 'Koral', 'Perfekcja', and magnesium doses (0, 45, 90 kg∙ha-1 of MgO) by constant fertilization with nitrogen (70 kg∙ha-1), phosphorus (80 kg∙ha-1 of P2O5), and potassium (100 kg∙ha-1 of K2O). In October harvested roots were stored for 6 months in the traditional natural mound. In the case of apple fruits seven different cultivars were used: 'Elstar', 'Gloster', 'Honeygold', 'Idared', 'Jonagored', 'Ligol', and 'Szampion', from the commercial Agricultural and Fruit Farm Klimkiewicz, Wtelno (Kuyavian-Pomeranian Voivodeship) using integrated horticulture production procedures. Apple fruits were stored under ULO conditions for 6 months (>2% O2, <2% CO2, 1.5-2.0°C and 95-96% RH). Susceptibility of commodities was evaluated directly after harvest and after the 6 months of storage using collorimetrically determination method at 475 nm. The evaluation of the enzymatic browning process in carrot roots and apple fruits revealed significant dependency on the used plant material (genus). Carrot roots were generally less susceptible to enzymatic browning compared to the apple fruits. Leaf application of magnesium fertilization, especially at the dose of 90 kg MgO per 1 ha inhibited the browning process. Storage time of 6 months in the mound promoted discoloration of carrot roots and thus negatively influenced their quality. In the case of apple fruits the appropriate choice of cultivar was most important for limiting the enzymatic browning process, which is especially important when selecting raw material for juice production. A low susceptibility to enzymatic browning was found in fruits of cv. 'Elstar', whereas the cv. 'Honeygold' was most susceptible. Changes in the susceptibility to the browning processes of fruit flesh after storage were statistically significant, however quite small in comparison to the values measured directly after harvest.
Yield amount, maturity stage, morphological as well as biological properties of carrot roots are cultivar-dependent. In 2007-2009, field experiments involving foliar fertilization of carrot with magnesium sulphate (acrid salts) on yield and selected yield constituents (dry matter, monosaccharides and total sugars) of carrot storage roots were conducted. Magnesium was applied in doses of 0, 45 and 90 kg MgO ha–1 in the form of 3% sprays during the intensive growth of carrot. The tested carrot belonged to five cultivars: medium- late Berjo and late Flacoro, Karotan, Koral and Perfekcja, all characterized by good shelf life. The yields of carrot storage roots depended on a cultivar and foliar fertilization with magnesium. The cultivar Flacoro gave the highest yield of 60.82 t ha–1 and cv. Karotan – lowest (51.40 t ha–1). The application of foliar magnesium fertilization during cultivation in the doses of 45 and 90 kg MgO ha–1 caused a significant increase of root yield of about 4.2 and 8.7%, respectively. The content of dry matter, reducing sugars and total sugars was determined in carrot roots immediately after harvest and after six months of storage. Regardless of the experimental factors, storage roots of cv. Karotan contained the highest amount of dry matter (138.7 g kg–1), reducing sugars (25.2 g kg–1) and of total sugars (76.8 g kg–1) based on fresh matter. Increasing fertilization with magnesium led to a significant increase in the content of all the analyzed constituents in carrot storage roots. The most successful was the dose of 45 kg MgO ha–1, which caused the highest significant increment in dry matter, reducing and total sugars. The six-month storage of carrot roots caused a 2.6% increase in dry matter and an 11.2% rise in total sugars, but decreased reducing sugars by 11.1% (mean results for all cultivars and fertilization variants).
Explants of apical buds of sandy everlasting Helichrysum arenarium L. Moench sterilized with calcium hypochlorite – Ca(OCl₂) were placed onto Murashige- -Skoog (MS) growth medium enriched with 1 mg∙dm⁻³ KIN (kinetin) in two consecutive passages. To optimize the procedure of in vitro micropropagation, the axillary shoots of Helichrysum arenarium L. Moench were transferred during the third passage onto 9 different combinations of MS medium without plant growth regulators (PGRs-free medium), with different concentrations of KIN (1.0, 3.0, 4.0, 5.0 mg∙dm⁻³) and KIN (1.0, 3.0, 4.0, 5.0 mg∙dm⁻³) with IAA (indole-3-acetic acid) (0.5 mg dm⁻³). The highest mean number of shoots (24.7) was observed on MS medium with 5 mg∙dm⁻³ KIN and 0.5 mg∙dm⁻³ IAA. The achieved branched shoots were rooted and acclimatized. Rhizogenesis was intensified by the presence of growth hormones: 0.5 mg∙dm⁻³ IAA or 0.5 mg∙dm⁻³ IBA (indole-3-butyric acid). At the stage of acclimation of plantlets the application of a water solution of MS salts (25%) for watering the plants increased the efficacy of plant acclimation from 56 to 75%.
The aim of the study was to investigate the effect of acidification and heat on the survival of Clostridium spores in third category animal by-products. Poultry minced meat and minced fish waste, contaminated with Clostridium sporogenes IW 1306 spore suspension, were subjected to the combined action of formic acid and high temperature of different values. To assess the efficiency of examined hygienization method, the spore number in biomass samples after acidification and heat treatment was estimated. For this purpose a 10-fold dilution series in peptone water was prepared and heat-treated at 80°C for 10 min. After cooling-down, one milliliter of each dilution was pour-plated onto DRCM medium solidified with agar. Statistical analyses were performed using the Statistica software. The results of the microbiological analysis show that lowering the pH to about 4 results in increasing the sensitivity of Clostridium sporogenes spores to a high – but not exceeding 100°C – temperature. The fastest inactivation of spores occurred after heating the acidified biomass at a temperature of 90°C. In the case of meat the 48-hour exposure to formic acid allowed the thermal inactivation of the spores within 15 minutes. The investigated method of hygienization may guarantee not only the inhibition of spores germination, but also enables their total elimination, reducing the epidemiological risk related to animal by-products handling.
Carrots cultivated in Poland are either sold as fresh vegetables or preserved. The quality of processed products does not depend only on the properly implemented technological process, but, also on the biological value of the initial raw material. The objective of the research study was to determine the impact of foliar fertilization of plants with a 3 % solution of magnesium amounting to: 0, 45, and 90 kg of MgO ha⁻¹ on the quality of roots of five carrot cultivars (‘Berio’, ‘Flacoro’, ‘Karotan’, ‘Koral’, and ‘Perfekcja’). Moreover, the effect of processing operations of carrot roots was determined as regards the quality of frozen and dried products as well as the quality of preserves in jars. The highest amounts of total carotenoids and vitamin C were found in the products produced from storage roots of carrot cultivated on small fields that were fertilized with a magnesium dose of 90 kg ha⁻¹. Irrespective of the applied processing operation, there were reported losses of carotenoids: 36.1 %, of vitamin C: 59.2 %, and of magnesium: 23.3 %. Under the freezing and preserving processes, the losses of carotenoids and vitamin C were the lowest, whereas under the drying process, the losses of those two components were the highest. The levels of those losses were, respectively: 28.6 and 23.6 % in the frozen products; 27.7 and 64.4 % in the preserves, and 51.9 and 89.6 % in the dried products. As for magnesium, the losses reported were quite different to those of the two previous compounds: the highest losses of 52.3 % occurred under the preservation process, whereas the lowest of 0.14 % under the drying process. The preserves produced from roots of carrots fertilized by the highest dose of magnesium were characterized by the highest nutritional value. Of the investigated carrot cultivars, it was found that the order of suitability for processing was ‘Karotan’, ‘Koral’, and ‘Perfekcja’.
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