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Due to importance and effectiveness of some entomopathogenic or insect parasitic nematodes in controlling of agricultural pests, or pests of non-agricultural plants, a study was conducted in order to identify the species of this group of nematodes in city of Tehran. As the result, three species belonging to the genus Oscheius were recovered in association with bark samples having the bark beetle galleries. Morphological and molecular data were provided for two recently recovered species of the genus, namely O. necromenus and O. onirici. Molecular data were also provided for a recently recovered isolate of O. tipulae. All three species were recovered in association with bark samples collected from dead trees in the city of Tehran. Morphological characters and morphometric data of the two aforementioned species are in accordance with the data given in their original descriptions. One recovered individual from a small bark sample characterized by its short body length was sequenced for its 28S and internal transcribed spacer (ITS) rDNA loci, and the results of BLAST search using the newly obtained partial sequences revealed that it belonged to O. tipulae. Molecular phylogenetic studies revealed recently sequenced Iranian populations of O. onirici and O. tipulae forming a clade with other isolates/populations of these species in ITS tree with maximal Bayesian posterior probability (BPP), and presently sequenced isolates of O. tipulae and O. necromenus form a clade with other isolates of these species in 28S tree. The two species O. onirici and O. necromenus were reported in Iran for the first time.
Ektaphelenchoides sylvestris sp. nov. is described and illustrated. The new species was recovered from the galleries of bark beetles from a dead Pinus sylvestris L. tree and characterized by females with 644-843 µm long body, lip region 7.5-9.0 µm wide, separated from the rest body with a shallow constriction, stylet 18-23 µm long, excretory pore 72-85 µm far from anterior end, postuterine sac short, 5-11 µm long and male absence. By having a short postuterine sac, the new species comes close to four known species of genus namely E. attenuata, E. musae, E. pini and E. winteri.
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