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1

Cadmium-induced alterations in morpho-physiology of two peanut cultivars differing in cadmium accumulation

100%
Zhang Z., Liu C., Wang X., Shi G.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 07
This study examines differences in the morphophysiological responses of low- and high-cadmium (Cd) accumulating peanut (Arachis hypogaea L.) cultivars to Cd stress. The biomass, Cd accumulation, leaf gas exchange, root morphology, root respiration, and hydraulic conductivity of Qishan 208 (low-Cd accumulator) and Haihua 1 (high-Cd accumulator) were determined via a hydroponic experiment. Exposure of peanut plants to 2 and 20 μM Cd considerably decreased their shoot biomass, net photosynthetic rate, transpiration rate, stomatal conductance, total root length, number of root tips, root respiration, and hydraulic conductivity. The root biomass, root surface area, and average diameter were unaffected by Cd exposure. The two cultivars differed in Cd accumulation and morphophysiological responses to Cd stress. Qishan 208 accumulated less Cd in plant tissues but was more sensitive to Cd stress than Haihua 1. The total root length, surface area, average diameter, number of root tips, and root respiration rate of Haihua 1 were significantly higher than those of Qishan 208. The well-developed root system and higher root respiration of Haihua 1 may be responsible for its high Cd accumulation capacity.
2

The regulating function of heterotrimeric G proteins in the immune system

100%
Wang Y., Li Y., Shi G.
Archivum Immunologiae et Therapiae Experimentalis
|
2013
|
tom 61
|
nr 4
3

Ship maneuvering prediction using grey box framwork via adaptive RM-SVM with minor rudder

100%
Mei B., Sun L., Shi G., Liu X.
Polish Maritime Research
|
2019
|
tom 26
|
nr 3
A grey box framework is applied to model ship maneuvering by using a reference model (RM) and a support vector machine (SVM) (RM-SVM). First, the nonlinear characteristics of the target ship are determined using the RM and the similarity rule. Then, the linear SVM adaptively fits the errors between acceleration variables of RM and target ship. Finally, the accelerations of the target ship are predicted using RM and linear SVM. The parameters of the RM are known and conveniently acquired, thus avoiding the modeling process. The SVM has the advantages of fast training, quick simulation, and no overfitting. Testing and validation are conducted using the ship model test data. The test case reveals the practicability of the RF-SVM based modeling method, while the validation cases confirm the generalization ability of the grey box framework
4

Differential gene expression analysis of a new Ogura CMS line and its maintainer in non-heading Chinese cabbage by cDNA-AFLP

88%
Zhang C., Qi L., Hou X., Shi G., Zhang J.
Acta Physiologiae Plantarum
|
2010
|
tom 32
|
nr 4
CMS is induced by the coordinated expression of certain mitochondrial and nuclear genes in flower development. Mitochondrial genes regulate manifestation of CMS, whereas nuclear genes regulate fertility phenotype and thus affect negatively. In this article, the buds of newly bred Ogura CMS of non-heading Chinese cabbage and its maintainer line as plant materials, genes differentially expressed transcripts were analyzed by cDNA-AFLP. Seventeen differently expressed genes were found in new Ogura CMS and nine genes in maintainer line. These genes were involved in energy metabolism, signal transduction, flower development, stress-related metabolism, transcription, etc. Expression patterns of three genes encoding BrCAM6, BrANK, BrTUB3 were verified by qRT-PCR in different organs and various stage flower buds of CMS and its maintainer line. The results revealed that two genes related to signal transduction, BrCAM6 and BrANK, were highly expressed in stamens and microspores of CMS than in maintainer line. As believed, these two genes involved in signal transduction of male sterile in CMS line. In comparison, BrTUB3 gene was accumulated in stamens and was expressed in significantly lower level in CMS line than in maintainer line. It expressed significantly lower in CMS than maintainer line after tetrad stage. This expression profile suggests that BrTUB3 played an important role in the development of the pollen, and may be closely related to male sterility.
5

Response of antioxidant activity to excess copper in two cultivars of Brassica campestris ssp. Chinensis Makino

88%
Li Y., Song Y., Shi G., Wang J., Hou X.
Acta Physiologiae Plantarum
|
2009
|
tom 31
|
nr 1
Changes in ascorbic acid content and antioxidant enzyme activities were investigated in non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) leaves of ‘Wutacai’ and ‘Erqing’ exposed to excess copper (Cu). Cu treatment reduced the fresh weight of shoot and root by 57% and 46% in ‘Wutacai’, and 60 and 54% in ‘Erqing’, respectively. The accumulation of copper in leaves was higher in ‘Wutacai’ than that in ‘Erqing’. Compared to the control, ascorbic acid (AsA) contents were significantly decreased after copper treatment in both cultivars, while they were higher in ‘Wutacai’ than in ‘Erqing’, which may explain the higher coppertolerance of ‘Wutacai’ with higher copper accumulation. The higher AsA contents of ‘Wutacai’ resulted from their lower activities of degrading enzymes, such as ascorbate oxydase (AAO) and ascorbate peroxidase (APX), as well as the increasing activity of dehydroascorbate reductase (DHAR) after copper treatment compared with ‘Erqing’. Copper stimulated superoxide dismutase (SOD) activity in both cultivars, but for catalase (CAT), there was little difference between both cultivars. Peroxidases (POD) activity was decreased after copper treatment in ‘Erqing’, while in ‘Wutacai’, it was significantly increased at 14 days, and POD activity was higher in ‘Wutacai’ than that in ‘Erqing’ at 21 and 28 days. Therefore, the induced increasing activity of POD in ‘Wutacai’ also played an important role in its copper tolerance.
6

Exceptionally well-preserved Early Cretaceous leaves of Nilssoniopteris from central Mongolia

76%
Herrera F., Shi G., Tsolmon G., Ichinnorov N., Takahashi M., Crane P. R., Herendeen P. S.
Acta Palaeobotanica
|
2018
|
tom 58
|
nr 2
7

Development of cost-effective tetra-ARMS PCR for detection of FecB genotype in sheep

76%
Guan F., Shi G., Wan P., Dai R., Tang H., Wang H., Luo Y.
Animal Science Papers and Reports
|
2014
|
tom 32
|
nr 3
The FecB gene has been shown to be crucial in reproduction in many sheep breeds. It is a single nucleotide polymorphism (SNP) located in the bone morphogenetic protein receptor IB (BMPRIB)gene. The current methods for genotyping the FecB mutation are either slow and laborious or expensive. In this report, a single-step amplification approach suitable for FecB genotyping method is described. Multiplex PCR was performed with four primers on the basis of tetraprimer amplification refractory mutation system PCR (tetra-primer ARMS PCR), then three FecB genotypes can be detected after electrophoresis. Genotyping results of the proposed multiplex PCR occurred to be in complete accordance with forced PCR-RFLP of all samples. It is a rapid and simple method for detection of FecB in a larger number of samples, and is suitable for other SNPs detection with specific primers even in most “low-tech” laboratories.
8

Physiological responses of Scirpus validus to nitrate stress

76%
Li X., Li H., Shi G., Xiao M., Li C., Xie H.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 1
9

The mitochondria mediate the induction of NOX1 gene expression by aldosterone in an ATF-1-dependent manner

64%
Fu Y., Shi G., Wu Y., Kawai Y., Qing T., Yue L., Xia Q., Miyamori I., Fan C.
Cellular and Molecular Biology Letters
|
2011
|
tom 16
|
nr 2
High aldosterone (Ald) levels can induce hypertrophy of vascular smooth muscle cells (VSMCs), which carries high risks of heart failure. A previous study showed that Ald induces hypertrophy of VSMCs by up-regulating NOX1, a catalytic subunit of NADPH oxidase that produces superoxides. However, the precise mechanism remains unknown. Diphenylene iodonium (DPI) is known as an inhibitor of complex I in the mitochondrial respiratory chain, and it was also found to almost completely suppress the induction of NOX1 mRNA and the phosphorylation of activating transcription factor (ATF-1) by PGF2α or PDGF in a rat VSMC cell line. In this study, we found that the Ald-induced phosphorylation of ATF-1 and NOX1 expression was significantly suppressed by DPI. Silencing of ATF-1 gene expression attenuated the induction of NOX1 mRNA expression, and over-expression of ATF-1 restored Ald-induced NOX1 expression. On the basis of this data, we show that the mitochondria mediate aldosterone-induced NOX1 gene expression in an ATF-1-dependent manner.
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