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Replication kinetics of neuropathogenic and non-neuropathogenic equine herpesvirus type 1 (EHV-1) strains in primary murine neurons and ED cell line

100%

Cymerys J., Slonska A., Brzezicka J., Tucholska A., Chmielewska A., Rola J., Malik P., Banbura M. W.

Polish Journal of Veterinary Sciences

2016

tom 19

nr 4

Equine herpesvirus type 1 (EHV-1) causes respiratory infections, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single-point mutation in DNA polymerase gene, resulting in an amino acid variation (N752/D752), is significantly associated with the neuropathogenic potential of EHV-1 strains. The aim of the study was to elucidate if there are any differences between neuropathogenic (EHV-1 26) and non-neuropathogenic (Jan-E and Rac-H) EHV-1 strains in their ability to infect neuronal cells. For the tested EHV-1 strains, cytopathic effect (CPE) was manifested by changed morphology of cells, destruction of actin cytoskeleton and nuclei degeneration, which led to focal degeneration. Moreover, EHV-1 26 strain caused fusion of the infected cells to form syncytia in culture. Real-time PCR analysis demonstrated that both neuropathogenic and non-neuropathogenic EHV-1 strains replicated in neurons and ED cells (equine dermal cell line) at a similar level. We can assume that a point mutation in the EHV-1 polymerase does not affect viral replication in this cell type.

Gniazdowanie brzegówki Riparia riparia i żołny Merops apiaster w Centralnej Polsce

81%

Boguszewski P., Boulton G., Kielan S., Malik P., Maniakowski M., Matyjasiak L., Nowicki M., Sieczak K., Tabor J., Tecza R., Wezyk M.

Kulon

2019

tom 24

Ograniczanie wyników

1 Kulon

1 Polish Journal of Veterinary Sciences

2 Malik P.

1 Banbura M. W.

1 Boguszewski P.

1 Boulton G.

1 Brzezicka J.

1 Chmielewska A.

1 Cymerys J.

1 Kielan S.

1 Maniakowski M.

1 Matyjasiak L.

1 Nowicki M.

1 Rola J.

1 Sieczak K.

1 Slonska A.

1 Tabor J.

1 Tecza R.

1 Tucholska A.

1 Wezyk M.

1 2019

1 2016

Wyniki wyszukiwania

Replication kinetics of neuropathogenic and non-neuropathogenic equine herpesvirus type 1 (EHV-1) strains in primary murine neurons and ED cell line

Gniazdowanie brzegówki Riparia riparia i żołny Merops apiaster w Centralnej Polsce