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Eleven new species of the family Mysmenidae occurring in China, in addition to one known species, Mysmenella gongi Yin, Peng et Bao, 2004, that was reported from Hunan Province, are diagnosed, described and illustrated. Three genera, i.e., Calodipoena, Mysmena, and Trogloneta, are firstly reported in China. Mysmenella pseudojobi sp. nov., where is found hitherto at the northest range of distribution of the family in China, and Trogloneta denticocleari sp. nov. is collected in caves. Mysmena spirala sp. nov. is collected from rainforest in Hainan Province. Calodipoena biangulata sp. nov., C. cornigera sp. nov., Mysmena zhengi sp. nov., M. rostella sp. nov., M. furca sp. nov., M. arcilongus sp. nov., Mysmenella menglunensis sp. nov. and Trogloneta speciosum sp. nov., from Xishuangbanna in Yunnan Province, are collected by fogging. Natural history and distributional map are provided.
The capacity of plants to achieve successful germination and early seedling establishment under high salinity is crucial for tolerance of plants to salt. The gaseous hormone ethylene has been implicated in modulating salt tolerance, but the detailed role of how ethylene modulates the response of early seedling establishment to salt is unclear. To better understand the role of the ethylene signal transduction pathway during germination and seedling establishment, an ethylene insensitive mutation (ein2-5) and an ethylene sensitive mutation (ctr1-1) of Arabidopsis were analyzed under saline conditions and compared with the wild type plant (Col-0) as control. High salinity (>100 mM NaCl) inhibited and delayed germination. These effects were more severe in the ethylene insensitive mutants (ein2-5) and less severe in the constitutive ethylene sensitive plants (ctr1-1) compared with Col-0 plants. Addition of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) or inhibitors of ethylene action implied that ethylene was essential for early seedling establishment under normal and saline conditions. Salt stress increased the endogenous concentration of hydrogen peroxide (H₂O₂) in germinating seeds and ACC reduced its concentration. Our results suggest that ethylene promotes germination under salinity by modulating the endogenous concentration of H₂O₂ in germinating seeds. These findings demonstrate that ethylene is involved in regulating germination as an initiator of the process rather than consequence, and that ethylene promotes germination by modulating the endogenous concentration of H₂O₂ in germinating seeds under salinity.
In order to assess the grain-size distribution and chemical speciation of heavy metals, 34 dust samples were collected in the city of Wuhu. Heavy metals concentrations in street dust of different districts varied greatly. Zn concentration in the traffic district, for example, is more than two times that of other districts, and Cu concentration in the industrial district is more than three times that of other districts. Zn mainly comes from traffic sources, and Cu mainly comes from industrial emissions sources. The highest concentrations of Cu and Cd appeared in the particle size of < 75 μm, and the other heavy metals appeared in the particle size of 96-75 μm. Heavy metals concentrations for different speciation appeared in an increasing trend with a reduction in particle size. Zn and Cd were mainly bound to acid-extractable state. Cr, V, Ni, and Co elements were mainly bound to a residual state. Cu and Pb were mainly bound to an organic state. The grain size fraction loads (GSF Load) of different chemical speciation were mainly high in fine particles. The biological mobility of heavy metals in street dust decreased in the order of Pb (91%) > Cu (85.6%) > Zn (85.24%) > Cd (78.3%) > Co (49.3%) > Ni (39.3%) > V (23.7%) > Cr (20.19%). Bioavailability of Cu emitted from industrial sources was high.
As an environmental factor, light influences the physiological functions and secondary metabolism of plants. However, the role of light in cotton fiber development and pigment biosynthesis has not yet been thoroughly explored. In this study, ovules of green cotton were cultured in vitro under dark and light conditions, and fiber and ovule growth parameters as well as fiber carbohydrate and cellulose contents and the expression of genes related to fiber development were investigated to elucidate the effect of light on fiber development. In addition, to investigate the effect of light on fiber pigment biosynthesis, the fiber flavonoid content and related gene expression were determined. The results demonstrated that the fiber length and the expression levels of fiber elongation genes under light culture were significantly lower than under dark culture, however, the ovule and fiber weight were significantly higher than under dark culture. The fiber developed under light culture had higher carbohydrate concentrations and carbohydrate transformation rate than under dark culture. Additionally, light culture exhibited higher cellulose contents and expression levels of cellulose biosynthesis genes compared with dark culture. In contrast, the pattern of the effect of light on flavonoid biosynthesis differed from that for cellulose biosynthesis. At 10 DAC (days after culture) and 20 DAC, the flavonoid contents and the expression levels of genes related to flavonoid biosynthesis were lower than under dark culture. However, the flavonoid contents and gene expression levels observed at 30 DAC and 40 DAC were higher in the light culture than in the dark culture. These results suggested that light hindered fiber elongation, but promoted carbohydrate accumulation and carbohydrate transformation, which resulted in fiber weight gain and increased cellulose accumulation in fibers. In addition, light inhibited flavonoid biosynthesis at early stage of fiber development, but promoted it at later stages. These findings provide the basis for intensive study of fiber development and flavonoids biosynthesis in green cotton.
Accumulation of reactive oxygen species (ROS) causes oxidative stress under adverse environmental conditions, such as salinity. Ethylene decreases accumulation of ROS induced by salinity, but the mechanism is still unclear. To examine the interactions between salinity and ROS accumulation and the possible role of ethylene metabolism in regulation, we used mutant ein2-5 in Arabidopsis with loss of function in EIN2. The mutant is compared to the wild-type Col-0, completely insensitivity to ethylene at the morphological, physiological and molecular levels. The oxidative responses of the wild type and mutant to salinity were compared. Loss-of-function of EIN2 enhanced sensitivity to salinity, implying that EIN2 is required for plant response to salinity. Furthermore, salinity resulted in accumulation of large amounts of ROS in ein2-5 seedlings when compared with Col-0, suggesting that the loss-of-function of EIN2 exaggerates oxidative stress induced by salinity. Activities of the antioxidant enzymes SOD, POD and CAT decreased significantly in ein2-5 under salinity when compared with Col-0 plants. The expression profiles of the genes Fe-SOD, PODs and CAT1, which code for ROS scavenging enzymes were severely decreased in ein2-5 under salinity compared with Col-0, suggesting that EIN2 was involved in regulating expression of these genes. Taken together, our results demonstrate that loss-of-function of EIN2 increased oxidative stress induced by salinity and that EIN2 is involved in modulating ROS accumulation, at least in part, by decreasing activities of ROS-scavenging enzymes.
An experiment was carried out to compare cadmium and copper absorption and mineral nutrition accumulation in pennisetum and tall fescue in order to select an appropriate grass to remediate Cd/Cu-contaminated soil and explore their detoxification mechanisms of contamination by mineral elements. The biomass remained constant in tall fescue under each Cd addition level and increased in pennisetum until Cu reached 500 μM, whereas they dramatically decreased as the Cu or Cd solution increased, which was concurrent with quadratic regression model analysis. The Cd/Cu concentrations in tall fescue were mostly accumulated in the roots and were much higher than those in pennisetum. The extracted amount of Cd in the shoots and the total Cu concentrations of pennisetum were higher than the corresponding values in tall fescue at every Cd/Cu addition level. Negative correlations were observed between Cd and shoot Ca, Cu, K, Mg, and Zn, and root Cu and Na of tall fescue and the root K of pennisetum. The Cu concentration was negatively correlated with K and positively correlated with Na in tall fescue and pennisetum under the Cu treatments. As the Cd/Cu concentration in solution increased, K/Na values were significantly decreased in the roots of tall fescue under Cu stress and pennisetum under Cd/Cu stress, whereas they increased in the roots of tall fescue under Cd addition. In summary, pennisetum exhibited the greater biomass and Cd/Cu extraction; indicating it as a candidate energy grass for phytoextraction. The adjustment capacity of grass for K and Na might relate to the tolerance to Cd/Cu.
The Arabidopsis Ethylene-Insensitive3 (EIN3) has received attention recently and has been shown to be involved in the regulation of multitude of responses ranging from biotic stress defense and development to hormone interaction. To better understand the roles of EIN3 in plants response to salinity stress during germination and postgermination development, seeds of two EIN3 deficient mutant and a EIN3 overexpression mutant of Arabidopsis were analyzed under salinity and compared with Col-0 as control. The results showed that the ein3-1eil1-1 double mutant (lacking EIN3 and EIN3-Like1) and ein3-1 (lacking EIN3) were hypersensitive to high salinity ([150 mM NaCl), while EIN3 overexpression mutant (EIN3ox) displayed enhanced tolerance, indicating that EIN3 plays important roles during seed germination under salinity. In addition, we also found that the two EIN3 deficient mutant seedlings accumulate high levels of hydrogen peroxide (H2O2), which was thought to be an inhibitor of germination under salinity before, suggesting that EIN3 may function as a negative regulator of reactive oxygen species metabolism in germinating seeds under salinity. Taken together, our studies provide insights that EIN3 promotes seed germination under salinity, at least in part, through modulating concentration of H2O2 in germinating seeds.
DNA methylation plays an important role in regulating gene expression in plants. In the experiment, we studied effects of cold on DNA methylation variation in upland cotton. Using the methylation-sensitive amplified polymorphism procedure, we chose 66 pairs of selective amplification primers to assess the status and levels of cytosine methylation. The hemimethylation of the external cytosine and the full methylation of the internal cytosine were scored. As a result, cold triggered the demethylation of hemimethylated or internally full methylated cytosine. With the prolongation of cold treatment, the demethylation loci increased and the methylation loci decreased. Nevertheless, this change could be reverted when cotton was subsequently recovered under normal temperature. In addition, 29 polymorphic bands that appeared in the electrophoretogram were sequenced. By homologous alignment analysis, most of these 29 fragments were identified as genes or DNA clones involved in abiotic stress response. The variation in methylation loci existed at both coding and non-coding regions. Furthermore, the expression of the abiotic stressrelated genes, GhCLSD (Seq21), GhARK (Seq22), GhARM (Seq15, Seq18, Seq19 and Seq21) and GhTPS (Seq8), were tested. The results revealed that cold treatment induced down-regulation of GhCLSD, GhARK and GhARM, but upregulated the expression of GhTPS. These changes were in accordance with the alteration of DNA methylation. Thus, cold may affect the gene expression via changing the methylation status in the cytosine nucleotide.
Brown cotton is a kind of naturally colored cotton. Because of less processing and little dying, it is more friendlier to environment than white cotton. For brown cotton, pigment accumulation in fiber is one of the most important characteristics. In this study, we selected a brown fiber line and a white fiber cultivar to determine the factor that affects the pigmentation in brown fiber. Accordingly, fibers were collected to verify the presence of PAs by p-dimethylaminocinnamaldehyde (DMACA) and toluidine blue O (TBO) staining. The PAs content and related genes expressions were determined. As a result, there were obvious differences on the aspect of PAs synthesis in fiber between white cotton and brown cotton. For white fiber, the PAs content reached maximum at 5 DPA, and then gradually decreased to zero. But for brown fiber, the PAs content was increased from 5 to 15 DPA stage, and reached the maximum at the 15 DPA stage, then gradually decreased from 15 to 40 DPA stage. On the contrary, in white cotton, PAs were synthesized in the whole developmental stage from 5 to 40 DPA. And PAs content in brown fiber were far more than that in white fiber, which may be the reason why the brown pigment accumulated in brown fiber.
This study investigates the effects of bird droppings on mercury pollution levels in soil, specifically on the speciation and total concentration of mercury (Hg) in soil from Tongli Wetland, East China. Thirty soil samples and four bird dropping samples were collected from Tongli Wetland along with fifteen eggshells and five feathers from Heron Branch birds. Results indicated that bird droppings affect local soil’s physic-chemical properties and Hg accumulation. Additionally, heron feathers were found to contain more total mercury (HgT) than their eggshells. Hg concentration in soil that is affected by bird dropping was determined to be 0.194±0.026 mg/kg; concentration in soil without bird droppings was 0.104±0.039 mg/kg. Therefore, HgT concentration in the former exceeded that of the latter (86.54%). Numerical analysis revealed that concentrations of water-soluble (F1), acid-soluble (F2), alkali-soluble (F3), hydrogen peroxide-soluble (F4), and residual mercury (F5) in soil that is affected by bird dropping were higher in soil that isn’t affected by bird droppings. However, concentrations of F1 remained mostly stable. We found a positive correlation between Hg concentrations in soil and excrement and concentrations of total carbon (Ctot), total nitrogen (Ntot), and hydrogen (H), in addition to an exponential proportional relationship between C/N and Hg/C. We concluded that fresh bird droppings in soil may promote mercury enrichment. Furthermore, bird droppings and highly decomposed humus increase soil HgT concentration when they remain in soil for an extended period of time.
Brown cotton is a kind of naturally colored cotton which accumulates brown pigment in fiber. In the experiment, the variations of DNA methylation and genes expression were studied during the development processes from 5 DPA to 25 DPA for brown fiber. By using the methylation-sensitive amplified polymorphism technique, we choose 66 pairs of selective-amplification primers to assess the status and levels of cytosine methylation. The hemimethylation of the external cytosine and the full methylation of the internal cytosine were scored. As a result, with the development of fiber from 5 DPA to 25 DPA, the ratios of the external hemimethylation and internal full methylation were gradually increased. In detail, at the stages of 5, 10, 15 and 25 DPA, the percentages of external hemimethylation were 8.29, 8.81, 9.77 and 10.09 %, the ratios of internal full methylation were 17.91, 19.20, 20.02 and 20.78 %, respectively. The development of brown fiber triggered the increase of methylated loci in the whole genome. For further analysis, we used cDNA-AFLP protocol to identify the specially expressed genes for further analysis. Totally, 30 polymorphic transcription-derived fragments (TDFs) were isolated and sequenced. By homologous alignment analysis, 19 TDFs were found similar with genes from cotton. Among them, the homologous genes of TDF5, TDF6, TDF10, TDF12, TDF17, TDF22, TDF23 and TDF25 were all from fiber. Furthermore, 13 TDFs were found to be homologous with reported functional genes.
Oral cancer remains a deadly disease worldwide. Lymph node metastasis and invasion is one of the causes of death from oral cancer. Elucidating the mechanism of oral cancer lymph node metastasis and identifying critical regulatory genes are important for the treatment of this disease. This study aimed to identify differentially expressed genes (gene signature) and pathways that contribute to oral cancer metastasis to lymph nodes. The GSE70604-associated study compared gene profiles in lymph nodes with metastasis of oral cancer to those of normal lymph nodes. The GSE2280-associated study compared gene profiles in primary tumor of oral cancer with lymph node metastasis to those in tumors without lymph node metastasis. There are 28 common differentially expressed genes (DEGs) showing consistent changes in both datasets in overlapping analysis. GO biological process and KEGG pathway analysis of these 28 DEGs identified the gene signature CCND1, JUN and SPP1, which are categorized as key regulatory genes involved in the focal adhesion pathway. Silencing expression of CCND1, JUN and SPP1 in the human oral cancer cell line OECM-1 confirmed that those genes play essential roles in oral cancer cell invasion. Analysis of clinical samples of oral cancer found a strong correlation of these genes with short survival, especially JUN expression associated with metastasis. Our study identified a unique gene signature – CCND1, JUN and SPP1 – which may be involved in oral cancer lymph node metastasis.
The enzyme strictosidine synthase (STR, EC: 4.3.3.2) plays a key role in the biosynthetic pathway of terpenoid indole alkaloid (TIA). It catalyzes the condensation of the tryptamine and secologanin to form 3α(S)-strictosidine, which is the common precursor of all TIAs. In this paper, a STR gene designated as DoSTR (GenBank: KX068707) was first cloned and characterized from Dendrobium officinale with rapid amplified cDNA ends method (RACE). DoSTR has a length of 1380bp with 1179bp open reading frame encoding 392 amino acids. BlastP analyses showed that its amino acid sequence was classified into Str_synth superfamily. qRT-PCR showed that DoSTR was expressed in all tissues tested, with a significantly higher level in flower and the lowest in stem. Four different treatments with MeJA, SA, ABA and AgNO₃, respectively, could induce the DoSTR expression to a different extent. And the effect of MeJA was the most obvious and transcript level of DoSTR induced by MeJA was 20.7 times greater than that of control at 48 hours after treatment. Furthermore, it was found that DoSTR was localized in vacuole through transient expression in tobacco. The characterization and expression of DoSTR can help in further studying the role of DoSTR in the biosynthesis of TIAs in D. officinale. This study may throw light on the alkaloid biosynthesis pathway of D. officinale.
 Phage ΦC31 integrase is a potential vector for the insertion of therapeutic genes into specific sites in the human genome. To understand the mechanism involved in ΦC31 integrase-mediated recombination, it is important to understand the interaction between the integrase and cellular proteins. Using a yeast two-hybrid system with pLexA-ΦC31 integrase as bait, we screened a pB42AD human fetal brain cDNA library for potential interacting cellular proteins. From the 106 independent clones that were screened, 11 potential interacting clones were isolated, of which one encoded C-terminal fragment of Sp100. The interaction between Sp100 and ΦC31 integrase was further confirmed by yeast mating and co-immunoprecipitation assays. The hybridization between a ΦC31 integrase peptide array and an HEK293 cell extract revealed that residues 81RILN84 in the N-terminus of ΦC31 integrase are responsible for the interaction with Sp100. Knocking down endogenous Sp100 with Sp100-specific siRNA increased ΦC31 integrase-mediated recombination but did not impact reporter gene expression. Therefore, endogenous Sp100 may interact with ΦC31 integrase and inhibit the efficiency of ΦC31 integrase-mediated recombination.
Thallium (Tl) is a typical trace metal of extreme high toxicity. As a concomitant element, Tl is widely found in various sulfide minerals and K-containing rock minerals. The outburst of Tl pollution in drinking water sources of the northern branch of the Pearl River in China as reported in 2010 has greatly aroused public concerns about Tl pollution in China. Apart from typical sources of Tl pollution such as Pb and Zn smelting and the mining and utilization of Tl-containing pyrite ores, the steel-making industry was discovered a new significant source that contributed to this Tl pollution incidence. Thallium contents in raw materials, fly ash and wastewater collected from a typical steel-making enterprise were determined by inductively coupled plasma mass spectrometry (ICP-MS). The results showed that Tl contents (0.02-1.03 mg/kg) are generally low in the raw materials, while fly ash samples have generally enriched Tl levels (1.31-6.45 mg/kg). Wastewater obtained from the dedusting process of the sintering furnace also exhibited excessive Tl levels (574-2130 μg/L). All these results suggested a possible release and gasification of Tl compounds from the raw materials under high temperatures (>800ºC) during the sintering processes, which were then accumulated in the flue gas and fly ash and washed into the wastewater by wet dedusting. Lime precipitation method is not effective for removing Tl from wastewater, since Tl mostly is present as dissolved Tl⁺ in the water. The study initiated a preliminary design of a fast and effective treatment method for Tl removal from Tl-containing industrial wastewater by using a deep oxidation system.
Angiopoietin-1 (Ang-1) belongs to a novel family of endothelial growth factors that function as ligands for an endothelialspecific receptor tyrosine kinase (Tie-2). The Ang-1/Tie-2 system may contribute to angiogenesis and vascular remodeling by mediating interactions of endothelial cells with smooth muscle cells and pericytes. The spatial distribution and temporal expression of Ang-1 and Tie-2 in the rat brain were studied following collagenase-induced intracerebral hemorrhage (ICH), by immunohistochemistry and reverse transcription-polymerase chain (RT-PCR) analysis, respectively. Immunohistochemical analysis revealed that some Ang-1 or Tie-2-positive dilated vessels resided around the hematoma and extended into the clot. RT-PCR analysis showed that Ang-1 and Tie-2 mRNA signal was detected at 2 days and persisted for 28 days after ICH. These findings suggest that ICH could lead to upregulation of Ang-1 and the receptor Tie-2 mRNA.
A semi-field test was conducted to assess the risk of exposure to fufenozide in a ditch and pond adjacent to an agricultural area. To support the investigation, a fast, highly selective, and sensitive method was developed to determine the residue of fufenozide in water, sediment, and soil through high performance liquid chromatography- tandem mass spectrometry. The recoveries were in the acceptable range of 85.6% to 99.3% in the three matrices, with the associated relative standard deviations at 1.2% to 7.8%. The results indicate that the surface water-sediment system could be exposed to fufenozide through runoff after application, which dissipated rapidly in the aquatic ecosystem. The toxicity exposure ratio showed no risk of fufenozide exposure to the fish in the aquatic ecosystem close to the agriculture field.
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