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Effect of disulfide and sulphydryl reagents on abortive and productive elongation catalyzed by Escherichia coli RNA polymerase

100%

Radlowski M., Job D.

Acta Biochimica Polonica

1994

tom 41

nr 4

The effect of disulfide and sulfhydryl reagents on the rate of abortive and productive elongation has been studied using Escherichia coli RNA polymerase holoenzyme and poly[d(A-T)] as template. In the presence of UTP as a single substrate and UpA as a primer, the enzyme catalyzed efficiently the synthesis of the trinucleotide product UpApU. Incubation of RNA polymerase with 1 mM 2-mercaptoethanoi resulted in a 5-fold increase of the rate of UpApU synthesis. In contrast, incubation of the enzyme with 1 mM 5,5'-dithio-bis(2-nitrobenzoic) acid resulted in a 6-fold decrease of the rate of abortive elongation. Determination of the steady state kinetic constants associated with UpApU synthesis disclosed that the disulfide and sulfhydryl reagents mainly affected the rate of UpApU release from the ternary transcription complexes and therefore influenced the stability of such complexes.

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A novel proteomic approach to analyse methionine oxidation reveals protein oxidation patterns in Arabidopsis seeds

81%

Meimoun P., Valot B., Job D., Bailly C.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2013

tom 94

nr 2

Ograniczanie wyników

1 Acta Biochimica Polonica

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2 Job D.

1 Bailly C.

1 Meimoun P.

1 Radlowski M.

1 Valot B.

1 2013

1 1994

Wyniki wyszukiwania

Effect of disulfide and sulphydryl reagents on abortive and productive elongation catalyzed by Escherichia coli RNA polymerase

A novel proteomic approach to analyse methionine oxidation reveals protein oxidation patterns in Arabidopsis seeds